A5049986054- Clinical Laboratory Practices and Quality Control
- Meta-analysis and systematic reviews
- Statistical Methods in Clinical Trials
- Adrenal Hormones and Disorders
- Folate and B Vitamins Research
- Blood groups and transfusion
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Health Systems, Economic Evaluations, Quality of Life
- Molecular Biology Techniques and Applications
- Hormonal Regulation and Hypertension
- Lipoproteins and Cardiovascular Health
- Thyroid Disorders and Treatments
- Metabolism and Genetic Disorders
- Liver Disease Diagnosis and Treatment
- Hemodynamic Monitoring and Therapy
- Cardiovascular Function and Risk Factors
- Analytical Chemistry and Chromatography
- Muscle metabolism and nutrition
- Platelet Disorders and Treatments
- Metabolomics and Mass Spectrometry Studies
- Medical Coding and Health Information
- Microfluidic and Capillary Electrophoresis Applications
- Chronic Kidney Disease and Diabetes
- Blood Pressure and Hypertension Studies
- Bacterial Identification and Susceptibility Testing
University of Dundee
1992-2025
Ninewells Hospital
2010-2024
National Health Service Scotland
2021
European School of Molecular Medicine
2018-2019
NHS Tayside
2018
Royal College of Physicians of Edinburgh
2007
Heart of England NHS Foundation Trust
2007
National Health Service
2007
Sanofi (United States)
2006
Heartlands Hospital
1993-2003
Abstract The risk of glucocorticoid-induced osteoporosis increases substantially with age but there is considerable individual variation. In recent studies we have shown that the effects glucocorticoids on bone are dependent autocrine actions enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1); expression 11β-HSD1 in osteoblasts (OBs) facilitates local synthesis active consequent osteoblastic proliferation and differentiation. Using primary cultures human OBs, now characterized...
Data on biological variation are used for many purposes in laboratory medicine but concern exists over the validity of data reported some studies. A critical appraisal checklist has been produced by a working group established European Federation Clinical Chemistry and Laboratory Medicine (EFLM) to enable standardised assessment existing future publications data. The identifies key elements be studies safe accurate effective transport sets across healthcare systems. is mapped domains minimum...
Abstract Background: Biological variation (BV) data have many fundamental applications in laboratory medicine. At the 1st Strategic Conference of European Federation Clinical Chemistry and Laboratory Medicine (EFLM) reliability limitations current BV were discussed. The EFLM Working Group on Variation is working to increase quality by developing a project establish biobank samples from healthy subjects be used produce high data. Methods: involved six laboratories (Milan, Italy; Bergen,...
Abstract BACKGROUND The European Federation of Clinical Chemistry and Laboratory Medicine Biological Variation Study (EuBIVAS) has been established to deliver rigorously determined data describing biological variation (BV) clinically important measurands. Here, EuBIVAS-based BV estimates serum electrolytes, lipids, urea, uric acid, total protein, bilirubin, direct glucose, as well their associated analytical performance specifications (APSs), are presented. METHOD Samples were drawn from 91...
Blood biomarkers have proven useful in Alzheimer's disease (AD) research. However, little is known about their biological variation (BV), which improves the interpretation of individual-level data.
Analytical performance specifications (APS) are typically established through one of three models: (i) outcome studies, (ii) biological variation (BV), or (iii) state-of-the-art. Presently, The APS can, for most measurands that have a stable concentration, be based on BV. BV APS, defined imprecision, bias, total allowable error and measurement uncertainty, applied to many different processes in the laboratory. When calculating it is important consider formulae, what setting they if suitable...
Abstract BACKGROUND The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Biological Variation Study (EuBIVAS) has been established to deliver rigorously determined biological variation (BV) indices. EuBIVAS BV for serum creatinine using the enzymatic alkaline picrate measurement methods. METHOD In total, 91 healthy individuals (38 males, 53 females; age range, 21–69 years) were bled 10 consecutive weeks at 6 laboratories. An equivalent protocol was followed each...
Abstract Context: The pathophysiological importance of glucocorticoids (GCs) is exemplified by patients with Cushing’s syndrome who develop hypertension, obesity, and insulin resistance. At a cellular level, availability GCs to the glucocorticoid mineralocorticoid receptors controlled isoforms 11β-hydroxysteroid dehydrogenase (11β-HSD). In liver adipose tissue, 11β-HSD1 converts endogenous, inactive cortisone active cortisol but also catalyzes bioactivation synthetic prednisone prednisolone....
We sought to develop estimates of biological variation (BV) for 9 enzymes in blood serum as part the European Biological Variation Study.Ninety-one healthy study participants (38 male and 53 female, 21-69 years old) were phlebotomized each 10 consecutive weeks at 6 laboratories. The same preanalytical sample-handling protocol was followed center before transport San Raffaele Hospital, Milan, Italy, analysis. Sera stored -80 °C analysis duplicate within a single run on an ADVIA 2400 Clinical...
The complete blood count (CBC) is used to evaluate health status in the contexts of various clinical situations such as anemia, infection, inflammation, trauma, malignancies, etc. To ensure safe application CBC, reliable biological variation (BV) data are required. study aim was define BVs CBC parameters employing a strict protocol.Blood samples, drawn from 30 healthy subjects (17 females, 13 males) once weekly for 10 weeks, were analyzed using Sysmex XN 3000 instrument. assessed normality,...
The European Biological Variation Study (EuBIVAS) was established to deliver rigorously determined data for biological variation (BV). Here, EuBIVAS-based BV estimates are provided α1-acid glycoprotein, α1-antitrypsin, albumin, β2-microglobulin, ceruloplasmin, complement component 3, 4, C-reactive protein (CRP), cystatin C, haptoglobin, IgA, IgG, IgM, soluble transferrin receptor (sTfR), and (Trf), together with their associated analytical performance specifications (APSs) reference change...
Abstract There is a need for standards generation and reporting of Biological Variation (BV) reference data. The absence affects the quality transportability BV data, compromising important clinical applications. To address this issue, international expert groups under European Federation Clinical Chemistry Laboratory Medicine (EFLM) have developed an online resource ( https://tinyurl.com/bvmindmap ) in form interactive mind map that serves as guideline researchers planning, performing...
Individual susceptibility to glucocorticoid-induced osteoporosis is difficult predict clinically. We recently characterized expression of 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in human osteoblasts. This enzyme generates active cortisol (or prednisolone) from inactive cortisone prednisone) and regulates glucocorticoid action vitro. We, thus, hypothesized that osteoblastic 11beta-HSD1 mediates osteoporosis. Twenty healthy males ingested 5 mg prednisolone twice daily for 7 d,...
<b>Aim:</b> Patients with retinal vein occlusions (RVO) are at increased risk of cardiovascular disease (CVD). The future CVD was determined using the Framingham algorithm and this estimate used to guide decisions about preventative treatment for in RVO patients. <b>Methods:</b> 107 unselected patients were studied. After excluding 18 because age, missing data, or pre-existing disease, calculated coronary heart risks (cCHDR) (cCVDR) on 89 remaining compared both standardised published...
Changes in levels of prolactin, growth hormone, luteinizing and follicle stimulating hormone serum, testosterone plasma, have been studied 38 patients with acute schizophrenic illnesses a 4-week double-blind comparison the 2 isomers flupenthixol placebo. Only prolactin showed changes which could be related either to clinical state or effects medication. Prolactin increased during treatment therapeutically active alpha-isomer but were unchanged inactive beta-isomer Although there was...
Background Laboratory investigations may be added to existing requests either automatically on the basis of algorithms (reflex testing) or by laboratory professionals (reflective testing). The clinical utility reflex and reflective testing is not fully established. We studied efficiency (number tests that needs make a diagnosis) effectiveness diagnoses) in selected biochemical scenarios. Methods Using fixed rules, we prospectively measured following scenarios initiators parentheses): (1)...