A5088665882- Identification and Quantification in Food
- Insect and Pesticide Research
- Bee Products Chemical Analysis
- Molecular Biology Techniques and Applications
- Traditional Chinese Medicine Analysis
- Meat and Animal Product Quality
- Biosensors and Analytical Detection
- Vector-Borne Animal Diseases
- Insect and Arachnid Ecology and Behavior
- Viral Infections and Immunology Research
- Ginseng Biological Effects and Applications
- Animal Disease Management and Epidemiology
Henan University of Science and Technology
2022-2024
Xuchang University
2024
To simultaneously detect sweetpotato and maize components in starch products, this study develops a rapid analysis method based on ladder-shape melting temperature isothermal amplification (LMTIA) coupled with proofreading enzyme-mediated probe cleavage (Proofman). Internal transcription spacer (ITS) gene sequences were targeted to design species-specific primers linked Proofman probes. The detection of duplex Proofman-LMTIA could be completed 20 min under the optimal 61 ℃. specificities ITS...
This study aims to establish a rapid identification method based on the Proofman-LMTIA technique for distinguishing between Panax quinquefolium and ginseng. By targeting specific 18S rDNA sequences, suitable primers Proofman probes labeled FAM or JOE were designed LMTIA. Initially, single-species-primer assays performed separately each ginseng type optimize reaction temperature, assess sensitivity specificity, determine detection limit. Subsequently, both sets of their corresponding combined...
Due to the unavailability of an effective vaccine or antiviral drug against African swine fever virus (ASFV), rapid diagnosis methods are needed prevent highly contagious fever.The objective this study was establish ladder-shape melting temperature isothermal amplification (LMTIA) assay for detection ASFV.LMTIA primers were designed with p72 gene ASFV as target, and plasmid pUC57 used clone gene. The LMTIA reaction system optimized positive control, performance compared that commercial...
Honey adulteration has been on the rise with increasing demand for high-quality honey. The ladders-shape melting temperature isothermal amplification (LMTIA) was a newly developed nucleic acid technique, and aim of this study to establish method combining LMTIA proofreading enzyme-mediated probe cleavage (Proof-man probe) detecting thaumatin-like protein (TLP) genes Brassica rapa var. oleifera in primers selected as target be designed, Proof-man reaction optimized, specificity, sensitivity,...
Honey adulteration has been on the rise with increasing demand for high-quality honey, and authentication of A. cerana honey is a major problem that current bee industry needs to solve. The aim this research was establish proofreading enzyme-mediated probe cleavage combined ladder-shape melting temperature isothermal amplification (Proofman-LMTIA) method Apis honey. LMTIA primers were designed target specific 18S rRNA sequence cerana, conditions Proofman-LMTIA optimized. specificity...