A5085301349- Identification and Quantification in Food
- Biosensors and Analytical Detection
- Enzyme Catalysis and Immobilization
- Phytochemicals and Antioxidant Activities
- Advanced Chemical Sensor Technologies
- Natural product bioactivities and synthesis
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Aquatic Ecosystems and Phytoplankton Dynamics
- Food composition and properties
- Bacteriophages and microbial interactions
- Marine and coastal ecosystems
- Microbial Metabolic Engineering and Bioproduction
- melanin and skin pigmentation
- Mycotoxins in Agriculture and Food
- Catalysis for Biomass Conversion
- Food Quality and Safety Studies
- Ginkgo biloba and Cashew Applications
- biodegradable polymer synthesis and properties
- Polysaccharides Composition and Applications
- Proteins in Food Systems
- Phytochemical compounds biological activities
- Phytochemistry and Biological Activities
- Biochemical and biochemical processes
- Essential Oils and Antimicrobial Activity
Xuchang University
2009-2024
Eastern Regional Research Center
2015
Agricultural Research Service
2015
Jiangnan University
2007-2009
Henan University of Technology
2006
Food adulteration is a serious problem all over the world. Establishing an accurate, sensitive and fast detection method important part of identifying food adulteration. Herein, sequence-specific ladder-shape melting temperature isothermal amplification (LMTIA) assay was reported to detect soybean-derived components using proofreading enzyme-mediated probe cleavage (named Proofman), which could realize real-time visual without uncapping. The results showed that, under optimal 57 °C,...
ABSTRACT Poly(lactic acid) (PLA) fibers of submicron sizes encapsulating allyl isothiocyanate (AITC) (P f A) were made and electrospun onto the surfaces PLA films. SEM examination confirmed fusion two phases to form a bilayered fiber‐grafted film after underwent air blowing water washing remove nongrafted fibers. The films A‐ g ‐film) retain mechanical properties PLA. release AITC from was temperature dependent. At temperatures lower than 4°C, incorporated remained within without losing...
A novel method, termed ladder-shape melting temperature isothermal amplification (LMTIA), was developed in this study. As a proof of concept, one pair primers or two pairs nested and thermostable DNA polymerase were employed to amplify the internal transcribed spacer Oryza sativa with curve. Our results demonstrated that LMTIA assay 50-fold more sensitive than LAMP same level specificity. The method has potential be used for prevention control emerging epidemics caused by different types pathogens.
To simultaneously detect sweetpotato and maize components in starch products, this study develops a rapid analysis method based on ladder-shape melting temperature isothermal amplification (LMTIA) coupled with proofreading enzyme-mediated probe cleavage (Proofman). Internal transcription spacer (ITS) gene sequences were targeted to design species-specific primers linked Proofman probes. The detection of duplex Proofman-LMTIA could be completed 20 min under the optimal 61 ℃. specificities ITS...
Dietary fiber is a carbohydrate-based polymer with significant health benefit, soybean residues are generally discarded in food industries during the production of milk and tofu. In this study, ultrasound-assisted alkali extraction technology was employed to extract insoluble dietary from residues. And physicochemical properties fibers were also studied. The optimal conditions for determined be solid liquid ratio 1:50, concentration 0.05 mol/L, ultrasound power 450 W, reaction time 10 min...
This study aims to establish a rapid identification method based on the Proofman-LMTIA technique for distinguishing between Panax quinquefolium and ginseng. By targeting specific 18S rDNA sequences, suitable primers Proofman probes labeled FAM or JOE were designed LMTIA. Initially, single-species-primer assays performed separately each ginseng type optimize reaction temperature, assess sensitivity specificity, determine detection limit. Subsequently, both sets of their corresponding combined...
Molecular detection of bacterial pathogens based on LAMP methods is a faster and simpler approach than conventional culture methods. Although different LAMP-based for pathogenic are available, systematic comparison these assays has not been performed. In this paper, we compared 12 in-house real-time with commercialized kit (Isothermal Master Mix) the Listeria monocytogenes, Salmonella spp, Staphylococcus aureus, Escherichia coli O157, E. O26, O45, O103, O111, O121, O145 Streptococcus...
The adulteration of the honey industry is serious, especially syrup method difficult to detect. To develop detection technology chaste with high fructose corn (HFCS), condition headspace solid-phase micro-extraction (HS-SPME) was optimized for extracting volatile compounds honey. HS-SPME conditions were selected optimization using Plackett Burman, steepest ascent and Box-Behnken. Volatile from adulterated HFCS analyzed by gas chromatography-mass spectrometry (GC-MS). M/Z RT pairs data...
Single copy thaumatin-like protein (TLP) genes and multiple internal transcribed spacer (ITS) from the nucleus of corn were selected as targets, designed screened primers. The impact target sequence selection on ladder-shape melting temperature isothermal amplification (LMTIA) detection components in edible oil aspects such specificity, sensitivity, stability studied. results showed that LMTIA system targeting TLP has an optimal reaction 58 °C can detect a minimum 100 pg/μL DNA, established...
Food authenticity has become increasingly important as a result of food adulteration. To identify the sweet potato starch noodles, ladder-shape melting temperature isothermal amplification (LMTIA) method determining cassava (Manihot esculenta Crantz) DNA in noodles was used. A set primers targeted at internal transcription spacer (ITS) designed, genomic extracted, LMTIA reaction optimized, and specificity primer verified with DNAs cassava, (Ipomoea batatas L.), Solanum tuberosum L., Zea mays...
Hydrothermal pretreatment is a green technology, which can effectively promote the degradation of biomass without adding any chemical reagent. In this study, hydrothermal on improving digestibility corncobs was investigated and effect changes structure corncob lignocellulose investigated. The composition amounts sugar content in hydrolysate enzymatic were determined. treatment destroyed caused different degrees degradation. With increasing temperature treatment, cellulose, hemicellulose...
A new rapid dual detection method was established to distinguish Bupleurum scorzonerifolium Willd. (BS) and chinense DC. (BC) simultaneously using the proofreading enzyme-mediated probe cleavage coupled with ladder-shape melting temperature isothermal amplification (proofman–LMTIA). The internal transcribed spacer (ITS) sequences of BS BC were selected as targets for designs proofman–LMTIA primers proofman fluorescence probes labeled FAM or JOE. reaction optimization, repeatability...
An ionic liquid (IL) system for the enzymatic epoxidation of soybean oil was studied. The effects active oxygen carriers (different fatty acids) and ILs ([Bmim]PF6 [Bmim]BF4) on were investigated. Response surface methodology (RSM) used to study optimize variables (reaction time, reaction temperature, molar ratio H2O2/C=C-bonds, acid/C=C-bonds) epoxy group content (EOC) epoxidized (ESO). Results showed that can be enhanced using tetradecanoic acid (C13H27COOH) as carrier [Bmim]PF6 medium....
A ladder-shape melting temperature isothermal amplification (LMTIA) assay was established and used to detect soybean components in edible oils.