A5014736338- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Pancreatic function and diabetes
- Liver physiology and pathology
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Graphene and Nanomaterials Applications
- Cancer Cells and Metastasis
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Electrohydrodynamics and Fluid Dynamics
- Liver Disease Diagnosis and Treatment
- Elasticity and Material Modeling
- Extracellular vesicles in disease
- Protease and Inhibitor Mechanisms
- Biochemical effects in animals
- Tissue Engineering and Regenerative Medicine
- Liver Disease and Transplantation
- Advanced Glycation End Products research
- Phagocytosis and Immune Regulation
- Hydrogels: synthesis, properties, applications
- Force Microscopy Techniques and Applications
- Blood properties and coagulation
- Organ Transplantation Techniques and Outcomes
Center for Life Sciences
2021-2024
Tsinghua University
2021-2024
The regeneration of hierarchical osteochondral units is challenging due to difficulties in inducing spatial, directional and controllable differentiation mesenchymal stem cells (MSCs) into cartilage bone compartments. Emerging organoid technology offers new opportunities for regeneration. In this study, we developed gelatin-based microcryogels customized using hyaluronic acid (HA) hydroxyapatite (HYP), respectively (denoted as CH-Microcryogels OS-Microcryogels) through vivo self-assembly...
This study revealed the enhanced therapeutic effects and mechanisms of Exendin-4–engineered MSCs for type 2 diabetes treatment.
The extracellular matrix (ECM) and cells are crucial components of natural tissue microenvironments, they both demonstrate dynamic mechanical properties, particularly viscoelastic behaviors, when exposed to external stress or strain over time. capacity modify the properties ECM is for gaining insight into development, physiology, pathophysiology living organisms. As an illustration, researchers have developed hydrogels with diverse compositions mimic native use them as substrates cell...
Physically crosslinked microgels (PCMs) offer a biocompatible platform for various biomedical applications. However, current PCM fabrication methods suffer from their complexity and poor controllability, due to reliance on altering physical conditions initiate gelation dependence specific materials. To address this issue, novel method is devised, which employs water transport-induced liquid-liquid phase separation (LLPS) trigger the intermolecular interaction-supported sol-gel transition...
Abstract Deposition of extracellular matrix (ECM) in the liver is an important feature cirrhosis. Recovery from cirrhosis physiologically challenging, partially due to ECM scar tissue showing resistance cell‐mediated degradation by secreted metalloproteinases (MMPs). Here, a ECM‐degradation screening system (CEDSS) vitro constructed for high‐throughput searching cells with tremendous ability. ECM‐degrading sinusoidal endothelial (dLSECs) are screened using CEDSS, which exhibit 17 times...
Nonunions and delayed unions pose significant challenges in orthopedic treatment, with current therapies often proving inadequate. Bone tissue engineering (BTE), particularly through endochondral ossification (ECO), emerges as a promising strategy for addressing critical bone defects. This study introduces mesenchymal stem cells overexpressing Exendin-4 (MSC-E4), designed to modulate remodeling via their autocrine paracrine functions. We established type I collagen (Col-I) sponge-based vitro...
Despite the rapid growth of clinical trials for cellular therapy worldwide, their success is still afflicted with formidable challenges demanding conceptual and technological overhaul. Pharmacology, which conventionally divided into pharmacokinetics (PK) pharmacodynamics (PD) in drug discovery have emerged as a prominent research direction to elucidate cell fate ensure efficacy safety therapeutic cells. Herein, we concisely present dilemmas therapies, concept pharmacology, advances...
Hematopoietic syndrome of acute radiation (h-ARS) is an illness resulted from the damage bone marrow (BM) microenvironment after exposure to radiation. Currently, clinical management h-ARS limited medication-assisted treatment, while there still no specific therapy for hematopoietic injury high-dose exposure. Our study aimed assemble biomimetic three-dimensional (3D) BM microniches by co-culture stem and progenitor cells (HSPCs) mesenchymal (MSCs) in porous, injectable viscoelastic...
Liver zonation, essential for diverse physiological functions, is lacking in existing organoid models, hindering their ability to recapitulate liver development and pathogenesis. Addressing this gap, we explored the feasibility of achieving zonated by co-culturing human embryonic stem cells (hESCs) derived hepatocytes with hESCs sinusoidal endothelial (LSECs) exhibiting characteristics either lobule's pericentral (PC) or periportal (PP) regions. Introducing LSECs variable WNT2 signaling...
Abstract Cell encapsulation technology, crucial for advanced biomedical applications, faces challenges in existing microfluidic and electrospray methods. Microfluidic techniques, while precise, can damage vulnerable cells, conventional methods often encounter instability capsule breakage during high‐throughput encapsulation. Inspired by the transformation of working state from unstable dripping to stable jetting triggered local electric potential, this study introduces a superimposed field...
Introduction Our study aims to provide a comprehensive overview of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in drug delivery research, focusing on the period between 2013 and 2023. Given increasing global interest this field, we utilized bibliometric tools explore publication trends, key contributors, thematic research clusters. Methods Data was collected from Web Science (WoS) database, an in-depth analysis conducted using VOSviewer. The encompassed bibliographic...
Liver fibrosis is typically caused by chronic viral hepatitis and, more recently, fatty liver disease associated with obesity. There are currently no approved drugs for cirrhosis, and transplantation limited donor scarcity, thus driving the investigation of novel therapeutic strategies. The development presents stage- zone-dependent characteristics that manifest as distinct dynamic changes during vascularization extracellular matrix (ECM) deposition. However, current cellular therapies do...
As the major components of natural tissue microenvironments, both extracellular matrix (ECM) and cells exhibit dynamic mechanical properties. In particular, viscoelastic interactions are influenced by external stresses strains over time. The tunability in properties ECM contributes significantly to development, physiology pathophysiology living organisms. For instance, hydrogels various compositions were synthesized, as cell culture substrate, mimick native ECMs. fate cultured can be...