- Ruminant Nutrition and Digestive Physiology
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Animal Nutrition and Physiology
- Rabbits: Nutrition, Reproduction, Health
- Turfgrass Adaptation and Management
- Agriculture Sustainability and Environmental Impact
- Agricultural and Food Production Studies
- Phytase and its Applications
- Meat and Animal Product Quality
- Plant and fungal interactions
- Agroforestry and silvopastoral systems
- Moringa oleifera research and applications
- Muscle metabolism and nutrition
- Animal Nutrition and Health
- Gout, Hyperuricemia, Uric Acid
- Bioenergy crop production and management
- Nuts composition and effects
- Agriculture and Rural Development Research
- Probiotics and Fermented Foods
- Garlic and Onion Studies
- Animal health and immunology
- Pharmacological Effects of Natural Compounds
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
Universidad Politécnica de Madrid
2015-2024
Escuela Nacional de Medicina del Trabajo
2023
University of Puerto Rico, Medical Sciences Campus
2020-2023
Universidad de León
2004-2013
Instituto de Ganadería de Montaña
2010-2013
West Virginia University
2009-2012
Institute of Environmental Assessment and Water Research
2009
Universidad Nacional de Colombia
2007
Universidad de León
1998-2006
Institut für Tier-, Natur- und Umweltethik
1995
Incubation trials were carried out with the rumen simulation technique (RUSITEC) to study effects of four forms N on growth ruminal micro-organisms and fermentation variables when an all-fibre basal diet was incubated. The consisted 10 g neutral-detergent fibre (NDF) from grass hay plus 2 NDF sugarbeet pulp. isolated soyabean protein, peptides, amino acids blended profile protein NH 3 as 4 Cl. Half daily supply infused Cl other half each treatments described. Non-NH (NAN) increased ( P =...
Six rumen-fistulated Merino sheep were used in a crossover design experiment to evaluate the effects of an exogenous fibrolytic enzyme preparation (12 g/d; ENZ), delivered directly into rumen, on diet digestibility, ruminal fermentation, and microbial protein synthesis. The contained endoglucanase xylanase activities. Sheep fed mixed grass hay:concentrate (70:30; DM basis) at daily rate 46.1 g/kg BW(0.75). Samples hay incubated situ rumen each measure NDF degradation. supplementation with...
The nutritive value of 26 agro-industrial by-products was assessed from their chemical composition, in vitro digestibility and rumen fermentation kinetics. By-products sugar beet, grape, olive tree, almond, broccoli, lettuce, asparagus, green bean, artichoke, peas, broad beans, tomato, pepper, apple pomace citrus were evaluated. Chemical kinetics varied largely across the by-products. Data subjected to multivariate principal component analyses (PCA). According a cluster analysis chart,...
The effects of disodium fumarate on microbial growth, CH 4 production and fermentation three diets differing in their forage content (800, 500 200 g/kg DM) by rumen micro-organisms vitro were studied using batch cultures. Rumen contents collected from four Merino sheep. Disodium was added to the incubation bottles achieve final concentrations 0, 8 mm-fumarate, 15 N used as a marker. Gas measured at regular intervals 0 120 h incubation. Fumarate did not affect ( P >0·05) any gas...
Six ruminally and duodenally cannulated sheep were used in a partially replicated 4 x Latin square to evaluate the effects of diets on microbial synthesis, populations, ruminal digestion. The experimental had forage concentrate ratios (F:C; DM basis) 70:30 (HF) or 30:70 (HC) with alfalfa hay (A) grass (G) as designated HFA, HCA, HFG, HCG. was based barley, gluten feed, wheat middlings, soybean meal, palmkern wheat, corn, mineral-vitamin premix proportions 22, 20, 13, 12, 5, 3%, respectively...
Two incubation runs were conducted with Rusitec fermenters to investigate the effects of three additive treatments (mixed fibrolytic enzymes from Trichoderma longibrachiatum (FE), disodium fumarate (FUM) and both additives (MIX)) on rumen microbial growth fermentation a grass hay:concentrate (600 : 400 g/kg DM) substrate. Each fermenter received daily 20 g substrate DM. Application rate (per was 34·3 endoglucanase, 0·57 exoglucanase, 24·7 xylanase 5·51 amylase units for FE 30 mg FUM. MIX...
This study was designed to analyze the chemical composition and in vitro rumen fermentation of eight seaweed species (Brown: Alaria esculenta, Laminaria digitata, Pelvetia canaliculata, Saccharina latissima; Red: Mastocarpus stellatus, Palmaria palmata Porphyra sp.; Green: Cladophora rupestris) collected Norway during spring autumn. Moreover, ruminal seventeen diets composed 1:1 oat hay:concentrate, without (control diet) or including seaweeds studied. The ash N contents were greater (p <...
Batch cultures of mixed rumen micro-organisms were used to study the effects different concentrations malate (Rumalato®; Norel & Nature S.A., Barcelona, Spain; composed disodium malate–calcium (0·16:0·84, w/w)) on fermentation four cereal grains (maize, barley, wheat and sorghum). Rumen contents collected from Merino sheep fed lucerne hay ad libitum supplemented with 300 g concentrate/d. Rumalato® was added incubation bottles achieve final 0, 4, 7 10 mM-MALATE. Gas production measured at...
Batch cultures of mixed rumen micro-organisms were used to study the effects different concentrations disodium fumarate on fermentation five concentrate feeds (maize, barley, wheat, sorghum and cassava meal). Rumen contents collected from four Merino sheep fed lucerne hay ad libitum supplemented with 300 g concentrate/d. Disodium was added incubation bottles achieve final 0, 4, 7 10 m -fumarate. In 17 h incubations, pH total volatile fatty acid production increased ( P <0·001) linearly...
Two incubation runs were carried out with a Rusitec system to investigate the effects of 2 exogenous pure cellulases on ruminal microbial growth and fermentation 70:30 grass hay:concentrate (DM basis) substrate. The substrate was sprayed buffer (control; pH = 6.5), cellulase from Trichoderma longibrachiatum (TRI), Aspergillus niger (ASP), or 1:1 mixture both (MIX) 24 h before being placed in fermenters. Enzymes applied at rate 30 endoglucanase units/g DM. Treating enzymes reduced NDF ADF...
The objective of this study was to evaluate the effects increasing doses [0 (control: CON), 20, 60, 180 and 540 mg/L incubation medium] garlic oil (GO) cinnamaldehyde (CIN) on in vitro ruminal fermentation two diets. Batch cultures mixed microorganisms were inoculated with fluid from four sheep fed a medium-concentrate diet (MC; 50 : alfalfa hay concentrate) or high-concentrate (HC; 15 85 barley straw concentrate). Diets MC HC representative those dairy fattening ruminants, respectively....