A5088022616- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Proteoglycans and glycosaminoglycans research
- Hemoglobinopathies and Related Disorders
- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- Infant Nutrition and Health
- Muscle metabolism and nutrition
- Metabolism and Genetic Disorders
- Neonatal Respiratory Health Research
- Advanced Proteomics Techniques and Applications
- Cystic Fibrosis Research Advances
- Protein purification and stability
- Iron Metabolism and Disorders
- Chemical Synthesis and Analysis
- Urinary and Genital Oncology Studies
- Bacterial Identification and Susceptibility Testing
- Probiotics and Fermented Foods
- Neonatal Health and Biochemistry
- Urologic and reproductive health conditions
- Lung Cancer Research Studies
- Lysosomal Storage Disorders Research
- Polyamine Metabolism and Applications
- Heavy Metal Exposure and Toxicity
- Trace Elements in Health
Centre Hospitalier Universitaire de Lille
2009-2019
Université de Lille
1988-2019
Université Lille Nord de France
2011-2014
Association Française pour le Dépistage et la Prévention des Handicaps de l’Enfant
2011
Institut de Biologie de Lille
2009-2011
Inserm
1987-2005
Centre Hospitalier Universitaire de Nantes
2005
Hôpital de la Timone
2005
Active Medicine (United Kingdom)
2005
Hôpital Albert Calmette
1998
Sickle Cell Disease (SCD) is an increasing global health problem and presents significant challenges to European care systems. Newborn screening (NBS) for SCD enables early initiation of preventive measures has contributed a reduction in childhood mortality from SCD. Policies methodologies NBS vary different countries, this might have consequences the quality clinical outcomes across Europe. A two-day Pan-European consensus conference was held Berlin April 2017 order appraise current status...
There is increasing evidence that inflammation may affect glycosylation and sulfation of various glycoproteins. The present study reports the effect tumor necrosis factor alpha (TNF-alpha), a proinflammatory cytokine, on glycosyl- sulfotransferases human bronchial mucosa responsible for biosynthesis Lewis x epitope its sialylated and/or sulfated derivatives, which are expressed in mucins. Fragments macroscopically normal were exposed to TNF-alpha at concentration 20 ng/ml. was shown increase...
Secreted human bronchial mucins, directly collected from macroscopically healthy mucosa, were prepared in the presence of six proteinase inhibitors, and analysed by electron microscopy. These mucins similar length distribution to molecules sputum [Slayter, Lamblin, Le Treut, Galabert, Houdret, Degand & Roussel (1984) Eur. J. Biochem. 142, 209-218], although they a little longer, their lengths ranging up about 1,650 nm. This corresponds an extended mucin peptide 450 kDa. In order compare...
Universal newborn screening for sickle cell diseases (SCDs) is not currently performed in many countries concerned by this public health problem. Owing to the technical and financial limitations of standard profiling methods (IEF coupled subsequent HPLC), ethnically targeted neonatal often preferred. Here, we demonstrate that MALDI-MS-based SCD could be considered as a potential method strategy universal because its high throughput, cost-effectiveness, sensitivity ability automatically...
A galactose 3-O-sulfotransferase activity able to transfer a sulfate group from adenosine 3'-phosphate 5'-phosphosulfate methyl galactosides or terminal N-acetyllactosamine-containing carbohydrate chains human respiratory mucins was characterized in microsomal fractions prepared mucosa. The reaction products, α- β-galactose 3-sulfate and three oligosaccharide alditols containing the sequence HSO3-3Galβ1-4GlcNAcβ1-6GalNAc-itol were identified by high performance anion-exchange...
A new method of urinary oligosaccharides identification by matrix-assisted laser desorption time-of-flight mass spectrometry is presented. The involves three steps: coupling the with 8-aminonaphthalene-1,3,6-trisulfonic acid; fast purification over a porous graphite carbon extraction column; and spectrometric analysis. Identification based on patterns values pseudomolecular ions observed. We report here in urines from patients Pompe disease, alpha beta mannosidoses, galacto-sialidosis, GM1...
Highly glycosylated regions of mucins, or glycopeptides, were obtained by proteolysis human bronchial mucins. They deglycosylated treatment with a trifluoromethane sulfonic acid/anisole mixture and subsequent solvolysis anhydrous liquid hydrogen fluoride. The resulting peptides then used to raise an immune serum in rabbit. This was localize the peptide precursors respiratory mucins within cells, using immunohistochemical method. Two main patterns labeling observed goblet cells: entire...
Human bronchial surface epithelial cells were maintained in secondary culture on a collagen gel substrate defined, serum-free medium. These conditions have previously been reported to promote mucous cell differentiation. After 3 wk culture, approximately 40% of the stained by an antibody directed against human respiratory mucin. Analysis media from cultured presence radioactive precursors [3H]glucosamine and [35S]sulfate revealed that secreted high molecular weight glycoproteins with...
Poly(A)-rich RNA was purified from a pool of five human tracheobronchial mucosa. After in vitro translation reticulocyte lysate and immunoprecipitation the translated products, using either polyclonal antiserum or monoclonal antibody to deglycosylated respiratory mucin peptides, products were characterized by SDS/PAGE. The precursors migrated as very large smear almost top resolving polyacrylamide gel an area corresponding molecular mass about 100 kDa. hybridization with cDNA probe TH 29...
Most screening programs for sickle cell disease (SCD) utilize isoelectric focusing (IEF) or high performance liquid chromatography (HPLC) to detect haemoglobin (Hb) variants. The first method is not automated and becomes too tedious when many samples have be investigated. aim of this work explore the capacity an capillary electrophoresis (CE) system, with full traceability, as a tool newborn SCD.The Capillarys neonat fast system has been developed by Sebia screening. We performed separate...
High-molecular-mass glycoconjugates are secreted by the continuous cell line MM-39, which has been obtained from cultured human tracheal gland cells transformed simian virus 40. They were purified on Sepharose® CL-4B and then two steps of density-gradient centrifugation. glycoproteins resistant to digestion hyaluronidase, chondroitin ABC lyase heparitinase obtained, in addition hyaluronic acid proteoglycans. susceptible β-elimination. contained polylactosaminoglycan chains as well...
Immunoblots of several urinary low-molecular-mass proteins can be very useful in investigations pathological proteinuria. However, use certain commercial antisera such procedures leads to artifacts corresponding nonspecific bands; e.g., immunoglobulins from nonimmunized rabbit serum may bind human proteins, and this binding is not inhibited by Triton X-100. We have developed a procedure improve the specificity detection low-Mr separated sodium dodecyl sulfate-polyacrylamide gel...
The reference methods used for sickle cell disease (SCD) screening usually include two analytical steps: a first tier differentiating haemoglobin S (HbS) heterozygotes, HbS homozygotes and β-thalassemia from other samples, confirmatory second tier. Here, we evaluated first-tier approach based on fully automated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) platform with sample processing, laboratory information management system NeoSickle®...
Previous research has shown that a MALDI-MS technique can be used to screen for sickle cell disease (SCD), and system combining automated sample preparation, analysis classification software is relevant approach first-line, high-throughput SCD screening. In order achieve “plug play” while detecting “non-standard” profiles might prompt the misclassification of sample, we have incorporated various sets alerts into decision support software. These included “biological alert” indicators...