A5030020118- MicroRNA in disease regulation
- RNA Research and Splicing
- Hepatitis C virus research
- RNA modifications and cancer
- Circular RNAs in diseases
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Cancer-related molecular mechanisms research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- interferon and immune responses
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- HIV/AIDS drug development and treatment
- Hepatitis B Virus Studies
- Viral Infections and Outbreaks Research
- RNA regulation and disease
- Infectious Encephalopathies and Encephalitis
- Liver Disease Diagnosis and Treatment
Justus-Liebig-Universität Gießen
2016-2024
Giessen School of Theology
2022
State Research Center of Virology and Biotechnology VECTOR
2012-2014
Novosibirsk State University
2012
The Coronaviridae is a family of positive-strand RNA viruses that includes SARS-CoV-2, the etiologic agent COVID-19 pandemic. Bearing largest single-stranded genomes in nature, coronaviruses are critically dependent on long-distance RNA-RNA interactions to regulate viral transcription and replication pathways. Here we experimentally mapped vivo interactome full-length SARS-CoV-2 genome subgenomic mRNAs. We uncovered network spanning tens thousands nucleotides. These reveal subgenomes adopt...
Circular RNAs (circRNAs) were recently described as a novel class of cellular RNAs. Two circRNAs reported to function molecular sponges, sequestering specific microRNAs, thereby de-repressing target mRNAs. Due their elevated stability in comparison linear RNA, may be an interesting tool medicine and biology. In this study, we provide proof-of-principle that can engineered microRNA sponges. As model system, used the Hepatitis C Virus (HCV), which requires microRNA-122 for its life cycle. We...
Abstract The new variant of concern (VOC) SARS-CoV-2, Omicron (B.1.1.529), is genetically very different from other VOCs. We compared with the preceding VOC Delta (B.1.617.2) and wildtype (wt) strain (B.1) respect to their interactions antiviral interferon (IFN-alpha/beta) response in infected cells. Our data indicate that IFN induction by low comparable wt, whereas showed an increased induction. However, exceeded both wt ability withstand state imposed IFN-alpha.
Natural circular RNAs have been found to sequester microRNAs and suppress their function. We used this principle as a molecular tool produced artificial RNA sponges in cell-free system by vitro transcription ligation. Formerly, we were able inhibit hepatitis C virus propagation applying decoy strategy against microRNA-122, which is essential for the viral life cycle. In another proof-of-principle study, microRNA-21, an oncogenic pro-proliferative microRNA. This slowed tumor growth 3D cell...
Abstract Naturally occurring circular RNAs efficiently impair miRNA functions. Synthetic may thus serve as potent agents for inhibition. Their therapeutic effect critically relies on (i) the identification of optimal targets, (ii) optimization decoy structures and (iii) development efficient formulations their use drugs. In this study, we extensively explored functional relevance miR-21-5p in cancer cells. Analyses transcriptomes reveal that is by far most abundant human cancers. Deletion...
Background: Hepatitis C virus (HCV) infects human liver hepatocytes, often leading to cirrhosis and hepatocellular carcinoma (HCC). It is believed that chronic infection alters host gene expression favors HCC development. In particular, HCV replication in Endoplasmic Reticulum (ER) derived membranes induces ER stress. How affects mRNA translation transcription at a genome wide level not yet known. Methods: We used Riboseq (Ribosome Profiling) analyze transcriptome translatome changes the...
SUMMARY The Coronaviridae is a family of positive-strand RNA viruses that includes SARS-CoV-2, the etiologic agent COVID-19 pandemic. Bearing largest single-stranded genomes in nature, coronaviruses are critically dependent on long-distance RNA-RNA interactions to regulate viral transcription and replication pathways. Here we experimentally mapped vivo interactome full-length SARS-CoV-2 genome subgenomic mRNAs. We uncovered network spanning tens thousands nucleotides. These reveal subgenomes...
The liver-specific microRNA-122 (miR-122) binds to two conserved binding sites in the 5′ UTR of hepatitis C virus (HCV) RNA. This was reported enhance HCV RNA replication, translation and stability. We have analysed miR-122/Argonaute 2 (Ago2) complexes these using anti-Ago2 co-immunoprecipitation radioactively labelled RNAs along with ectopic miR-122 HeLa cells. Our results show that target can be addressed separately. When both were simultaneously, we observed a synergistic miR/Ago2...
The HIV-1 epidemic in Russia is dominated by the former Soviet Union subtype A (A(FSU)) variant, but other genetic forms are circulating country. One recently described CRF63_02A1, derived from recombination between a CRF02_AG variant Central Asia and A(FSU), which has spread Novosibirsk region, Siberia. Here we phylogenetically analyze pol env segments 24 samples region collected 2013, with characterization of three new near full-length genome CRF63_02A1 sequences, estimate time most recent...
Multiple host factors are known to play important roles in hepatitis C virus (HCV) replication, immune responses induced by HCV infection, or processes that facilitate escape from clearance, while yet only few studies examined the contribution of long non-coding RNAs (lncRNAs/lncRs). Using microarrays, we identified lncRNAs with altered expression levels replicating Huh-7.5 hepatoma cells. Of these, lncR 8(Lnc-ITM2C-1/LOC151484) was confirmed quantitative real-time PCR (qRT-PCR) be...
Abstract The new variant of concern (VOC) the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Omicron (B.1.1.529), is genetically very different from other VOCs. We compared with preceding VOC Delta (B.1.617.2) and wildtype strain (B.1) respect to their interactions antiviral type I interferon (IFN-alpha/beta) response in infected cells. Our data indicate that has gained an elevated capability suppress IFN-beta induction upon infection better withstand state imposed by...
Hepatitis C virus (HCV) infects liver cells and often causes chronic infection, also leading to cirrhosis cancer. In the cytoplasm, viral structural non-structural (NS) proteins are directly translated from plus strand HCV RNA genome. The NS3 NS5B constitute replication complex that is required for genome via a minus antigenome. most C-terminal protein in replicase, which needs initiate antigenome synthesis at very 3′-end of strand. Using ribosome profiling replicating full-length infectious...
Abstract Rift Valley fever virus (RVFV) is a mosquito-borne zoonotic pathogen. Its RNA genome consists of two negative-sense segments (L and M) with one gene each, ambisense segment (S) opposing genes separated by the noncoding “intergenic region” (IGR). These vRNAs complementary cRNAs are encapsidated nucleoprotein (N). Using iCLIP2 (individual-nucleotide resolution UV crosslinking immunoprecipitation) to map all N-vRNA N-cRNA interactions, we detect N coverage along L M segments. However,...