Abstract In Drosophila , the JNK pathway eliminates by apoptosis aberrant cells that appear in development. It also performs other functions associated with cell proliferation, but analysis of latter is hindered pro-apoptotic activity. We report response apoptosis-deficient to transient activation and show it causes persistent function during rest As a consequence, there continuous activity downstream pathways JAK/STAT, Wg Dpp, which results tumour overgrowths. oncogenic potential Ras-MAPK...

We have found that the γ2 subunit of GABA A receptor (γ2-GABA R) specifically interacts with protocadherin-γC5 (Pcdh-γC5) in rat brain. The interaction occurs between large intracellular loop γ2-GABA R and cytoplasmic domain Pcdh-γC5. In brain extracts, Pcdh-γC5 coimmunoprecipitates Rs. cotransfected HEK293 cells, promotes transfer to cell surface. previously shown that, cultured hippocampal neurons, endogenous forms clusters, some which associate GABAergic synapses. Overexpression neurons...

Programmed cell death (apoptosis) is a homeostasis program of animal tissues designed to remove cells that are unwanted or damaged by physiological insults. To assess the functional role apoptosis, we have studied consequences subjecting Drosophila epithelial defective in apoptosis stress genetic perturbations normally cause massive death. We find many those acquire persistent activity JNK pathway, which drives them into senescent status, characterized arrest division, hypertrophy, Senescent...

We have isolated, from a rat brain cDNA library, clone corresponding to 2779-bp encoding novel splice form of the glutamate receptor interacting protein-1 (GRIP1). call this 696-amino acid GRIP1c 4-7 differentiate it longer forms GRIP1a/b containing seven PDZ domains. The four domains are identical GRIP1a/b. also contains 35 amino acids at N terminus and 12 C that different In transfected HEK293 cells, majority was associated with plasma membrane. interacted GluR2/3 subunits...

A new full-length cDNA (ZFOR4) that encodes an opioid receptor has been isolated from the teleost zebrafish. The encoded polypeptide is 375 amino acids long and shows high sequence similarity to other delta-opioid receptors, including ZFOR1, zebrafish previously characterized by us. In situ hybridization studies have revealed ZFOR4 mRNA highly expressed in particular brain areas coincide with expression of species. Pharmacological analysis specific saturable binding [(3)H] diprenorphine,...

We have found that the large intracellular loop of γ2 GABAA receptor (R) subunit (γ2IL) interacts with RNF34 (an E3 ubiquitin ligase), as shown by yeast two-hybrid and in vitro pulldown assays. In brain extracts, co-immunoprecipitates assembled GABAARs. co-transfected HEK293 cells, reduces expression GABAAR increasing ratio ubiquitinated/nonubiquitinated γ2. Mutating several lysines γ2IL into arginines makes resistant to RNF34-induced degradation. also when α1 β3 subunits are co-assembled...

Morphogenesis of epithelial tissues relies on the interplay between cell division, differentiation and regulated changes in shape, intercalation sorting. These processes are often studied individually relatively simple epithelia that lack complexity found during organogenesis when these might all coexist simultaneously. To address this issue, we making use developing fly retinal neuroepithelium. Retinal morphogenesis a coordinated sequence interdependent morphogenetic events includes apical...

In Drosophila photoreceptors, Rhodopsin 1 (ninaE, Rh1) is required for proper morphogenesis and maintenance of the apical light-gathering organelle, rhabdomere. It has been proposed that Rh1, coupled to Rho GTPases Rac1 Cdc42, promotes a sub-rhabdomeric F-actin meshwork or rhabdomere terminal web (RTW). The RTW provides mechanical support microvilli likely guide Rab11-dependent delivery Rh1-rich membrane from trans Golgi network. However, nature function molecular pathway involved in remains...

The Drosophila body comprises a central part, the trunk, and outgrowths of appendages. Much is known about appendage regeneration, but little trunk. As wing imaginal disc contains trunk component, notum, appendage, we have investigated response to ablation these two components. We find that, in contrast with strong regenerative wing, notum does not regenerate. Nevertheless, elimination primordium elicits proliferative cells, they do regenerate wing; form duplicate. Conversely, cells cannot...

In Drosophila epithelial cells, apical exclusion of Bazooka (the Par3 protein) defines the position zonula adherens (ZA), which demarcates and lateral membrane allows cells to assemble into sheets. Here, we show that small GTPase Rap1, its effector Canoe (Cno) Cdc42 kinase Mushroom bodies tiny (Mbt), converge in regulating morphogenesis by coupling stabilization junction (AJ) protein E-Cadherin retention at ZA. Furthermore, our results localization Cno Mbt ZA is interdependent, indicating...

Abstract We cloned two novel alternatively‐spliced mRNA isoforms of glutamate receptor interacting protein 1 (GRIP1) which we named GRIP1d and GRIP1e 4–7. is a 135 kDa, 7‐PDZ‐domain variant GRIP1, containing the 12 amino acid C‐terminus originally described for 4‐PDZ‐domain GRIP1c 4–7 75 kDa GRIP1a/b. Northern blots indicated that 5.1 kb long abundant in brain. An antibody to also recognized an protein, consistent with predicted size GRIP1d. Similarly, GRIP1a/b low abundance...

Regeneration is a response mechanism aimed to restore tissues that have been damaged. We are studying in the wing disc of Drosophila regenerative dose Ionizing Radiation kills over 35% cells distributed all disc. After such treatment discs able normal size, indicating there repairs generalised damage. tested role JNK, JAK/STAT and Wg pathways, known be required for regeneration after localised damage find irradiation size compensation absence function these they not necessary compensation....

Summary Programmed cell death (apoptosis) is a homeostasis program of animal tissues designed to remove cells that are unwanted or damaged by physiological insults. To assess the functional role apoptosis we have studied consequences subjecting Drosophila epithelial defective in stress genetic perturbations normally cause massive death. We find many those acquire persistent activity JNK pathway, which drives them into senescent status, characterized arrest division, hypertrophy, Senescent...

Abstract The epithelial Zonula adherens (ZA) is a main adhesion compartment that enables organogenesis by allowing cells to assemble into sheets. How ZA assembly regulated during cell morphogenesis not fully understood. We show morphogenesis, the function of small GTPase Rap1 and F-actin binding protein AF6/Cno are both linked P21-activated kinase Pak4/Mbt. find Mbt regulate each other’s localization at cooperate in promoting ECadherin stabilization. During this process Cno regulates...

Abstract Regeneration is a response mechanism to restore tissues that have been damaged or lost by mechanical physiological insults. We are studying in the wing imaginal disc of Drosophila regenerative high dose Ionizing Radiation (IR), which we estimate kills at least 35% cells. After such treatment irradiated discs able normal size and shape, indicating there replace tested role Jun N-terminal Kinase (JNK), Janus (JAK/STAT) Wingless (Wg) pathways, shown promote cell proliferation...