A5001169450- Neuroscience and Neuropharmacology Research
- Invertebrate Immune Response Mechanisms
- CAR-T cell therapy research
- Nicotinic Acetylcholine Receptors Study
- Insect symbiosis and bacterial influences
- Ion channel regulation and function
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- Aquaculture disease management and microbiota
- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- Retinal Development and Disorders
- Neurogenesis and neuroplasticity mechanisms
- Calcium signaling and nucleotide metabolism
- Wnt/β-catenin signaling in development and cancer
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Genetic Neurodegenerative Diseases
- Crustacean biology and ecology
- Virus-based gene therapy research
- Axon Guidance and Neuronal Signaling
- Muscle Physiology and Disorders
- Genetics and Neurodevelopmental Disorders
University of California, Los Angeles
2019-2023
University of Connecticut
2009-2021
Children’s Institute
2020
UCLA Health
2018
National Taiwan Ocean University
2006-2009
Significance Commercial strategies to deliver biomolecular cargo ex vivo (e.g., electroporation, lipofection) clinically relevant cell lines are limited by toxicity, cost, and throughput. These technical limitations have inhibited development of these technologies into streamlined clinical platforms for manufacturing gene-modified stem cells cancer immunotherapies. Here, we demonstrate an acoustofluidic platform capable delivering plasmids with high throughput human T lymphocytes, peripheral...
Collybistin promotes submembrane clustering of gephyrin and is essential for the postsynaptic localization γ-aminobutyric acid type A (GABA(A)) receptors at GABAergic synapses in hippocampus amygdala. Four collybistin isoforms are expressed brain neurons; CB2 CB3 differ C terminus occur with without Src homology 3 (SH3) domain. We have found that transfected hippocampal neurons, all (CB2(SH3+), CB2(SH3-), CB3(SH3+), CB3(SH3-)) target to concentrate postsynapses. Moreover, non-transfected...
We have found that the γ2 subunit of GABA A receptor (γ2-GABA R) specifically interacts with protocadherin-γC5 (Pcdh-γC5) in rat brain. The interaction occurs between large intracellular loop γ2-GABA R and cytoplasmic domain Pcdh-γC5. In brain extracts, Pcdh-γC5 coimmunoprecipitates Rs. cotransfected HEK293 cells, promotes transfer to cell surface. previously shown that, cultured hippocampal neurons, endogenous forms clusters, some which associate GABAergic synapses. Overexpression neurons...
We have found that the large intracellular loop of γ2 GABAA receptor (R) subunit (γ2IL) interacts with RNF34 (an E3 ubiquitin ligase), as shown by yeast two-hybrid and in vitro pulldown assays. In brain extracts, co-immunoprecipitates assembled GABAARs. co-transfected HEK293 cells, reduces expression GABAAR increasing ratio ubiquitinated/nonubiquitinated γ2. Mutating several lysines γ2IL into arginines makes resistant to RNF34-induced degradation. also when α1 β3 subunits are co-assembled...
We studied the effect of clonal overexpression neuroligin 3 (NL3) or 2 (NL2) in adult rat cerebral cortex following utero electroporation (IUEP) at embryonic stage E14. Overexpression NL3 leads to a large increase vesicular gamma-aminobutyric acid (GABA) transporter (vGAT) and glutamic decarboxylase (GAD)65 GABAergic contacts that overexpressing neurons receive. NL2 produced similar but lesser extent. In contrast, after IUEP does not affect glutamate 1 (vGlut1) glutamatergic...
Collybistin (CB) is a guanine nucleotide exchange factor selectively localized to γ-aminobutyric acid (GABA)ergic and glycinergic postsynapses. Active CB interacts with gephyrin, inducing the submembranous clustering postsynaptic accumulation of which scaffold protein that recruits GABAA receptors (GABAARs) at postsynapse. expressed or without src homology 3 (SH3) domain. We have previously reported effects on GABAergic synapses acute overexpression CBSH3− CBSH3+ in cultured hippocampal (HP)...
The recruitment of inhibitory GABA-A receptors to neuronal synapses requires a complex interplay between receptors, neuroligins, the scaffolding protein gephyrin and GDP-GTP exchange factor collybistin. Collybistin is regulated by protein-protein interactions at N-terminal SH3 domain, which can bind neuroligins 2/4 GABA-AR α2 subunit. also harbours RhoGEF domain mediates with catalyses on Cdc42. Lastly, collybistin has pleckstrin homology (PH) binds phosphoinositides, such as...
Abstract It has been proposed that the combinatorial expression of γ‐protocadherins (Pcdh‐γs) and other clustered protocadherins (Pcdhs) provides a code molecular identity individuality to neurons, which plays major role in establishment specific synaptic connectivity formation neuronal circuits. Particular attention directed Pcdh‐γ family, for experimental evidence derived from Pcdh‐γ‐deficient mice shows they are involved dendrite self‐avoidance, synapse development, dendritic...
Abstract Collybistin (CB) is a guanine nucleotide exchange factor (GEF) selectively localized at GABAergic and glycinergic postsynapses. Analysis of mRNA shows that several isoforms collybistin are expressed in the brain. Some have SH3 domain (CBSH3+) some no (CBSH3−). The CBSH3+ mRNAs predominantly over CBSH3−. However, an immunoblot study mouse brain homogenates, only protein were detected, proposing CBSH3− might not be expression or lack important issue because has strong effect promoting...
Intracellular delivery technologies that are cost-effective, non-cytotoxic, efficient, and cargo-agnostic needed to enable the manufacturing of cell-based therapies as well gene manipulation for research applications. Current capable delivering large cargoes, such plasmids CRISPR-Cas9 ribonucleoproteins (RNPs), plagued with high costs and/or cytotoxicity often require substantial specialized equipment reagents, which may not be available in resource-limited settings. Here, we report an...