A5088900002- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Gold and Silver Nanoparticles Synthesis and Applications
- Lipid Membrane Structure and Behavior
- Electrohydrodynamics and Fluid Dynamics
- Advanced biosensing and bioanalysis techniques
- Nanowire Synthesis and Applications
- Semiconductor materials and devices
- Laser-Ablation Synthesis of Nanoparticles
- Ultrasound and Cavitation Phenomena
- 3D Printing in Biomedical Research
- Molecular Junctions and Nanostructures
- Microbial Inactivation Methods
- Biosensors and Analytical Detection
University of California, Los Angeles
2020
California NanoSystems Institute
2020
Significance Commercial strategies to deliver biomolecular cargo ex vivo (e.g., electroporation, lipofection) clinically relevant cell lines are limited by toxicity, cost, and throughput. These technical limitations have inhibited development of these technologies into streamlined clinical platforms for manufacturing gene-modified stem cells cancer immunotherapies. Here, we demonstrate an acoustofluidic platform capable delivering plasmids with high throughput human T lymphocytes, peripheral...
Self-assembled monolayers (SAMs) of carborane isomers with different dipole moments passivate germanium to modulate surface work function while maintaining chemical environment and energy. To identify head groups capable monolayer formation on surfaces, we studied thiol-, hydroxyl-, carboxyl-terminated carboranes. These films were successfully formed carboxylic acid instead the archetypal thiol, suggesting that cluster significantly affects headgroup reactivity. Film characterization...
Gold nanostars (AuNSTs) are biocompatible, have large surface areas, and characterized by high near-infrared extinction, making them ideal for integration with technologies targeting biological applications. We developed a robust simple microfluidic method the direct growth of anisotropic AuNSTs on oxide substrates including indium tin glass. The synthesis was optimized to yield anisotropy, branching, uniformity, density in batch systems optimal light-to-heat conversion upon laser...
Innovative technologies for intracellular delivery are ushering in a new era gene editing, enabling the utilization of patient's own cells stem cell and immunotherapies. In particular, cell-squeezing platforms provide unconventional forms delivery, deforming through microfluidic constrictions to generate transient pores enable effective diffusion biomolecular cargo. While these devices promising gene-editing platforms, they require frequent maintenance due accumulation cellular debris,...