A5041228413- Molecular Junctions and Nanostructures
- Force Microscopy Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Lipid Membrane Structure and Behavior
- Quantum and electron transport phenomena
- RNA Interference and Gene Delivery
- Nanofabrication and Lithography Techniques
- Quantum-Dot Cellular Automata
- Neuroscience and Neural Engineering
- Advancements in Photolithography Techniques
- Nanowire Synthesis and Applications
- Carbon Nanotubes in Composites
- Genomics and Chromatin Dynamics
- Microbial Inactivation Methods
- Blood properties and coagulation
- Nanoparticle-Based Drug Delivery
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Graphene and Nanomaterials Applications
- Semiconductor materials and devices
- Advanced Electron Microscopy Techniques and Applications
- Ultrasound and Cavitation Phenomena
- Complement system in diseases
- Advanced Drug Delivery Systems
- Nuclear Structure and Function
University of California, Los Angeles
2017-2022
California NanoSystems Institute
2017-2022
University of California, Berkeley
2017-2020
International Institute for Nanotechnology
2017
Kavli Energy NanoScience Institute
2017
Lawrence Berkeley National Laboratory
2017
Northwestern University
2017
Nanyang Technological University
2016
Significance Commercial strategies to deliver biomolecular cargo ex vivo (e.g., electroporation, lipofection) clinically relevant cell lines are limited by toxicity, cost, and throughput. These technical limitations have inhibited development of these technologies into streamlined clinical platforms for manufacturing gene-modified stem cells cancer immunotherapies. Here, we demonstrate an acoustofluidic platform capable delivering plasmids with high throughput human T lymphocytes, peripheral...
Deficiencies in either lamin B1 or B2 cause both defective migration of cortical neurons the developing brain and reduced neuronal survival. The abnormality is explained by a weakened nuclear lamina that interferes with nucleokinesis, translocation process required for migration. In contrast, explanation impaired survival poorly understood. We hypothesized forces imparted on nucleus during result membrane (NM) ruptures, causing interspersion cytoplasmic contents-and ultimately cell death. To...
With mounting evidence that nanomaterials can trigger adverse innate immune responses such as complement activation, there is increasing attention to the development of strategies mask complement-activating properties nanomaterials. The current gold standard reduce activation covalent attachment polymer coatings on nanomaterial surfaces, even though this strategy provides only moderate protection against activation. Akin protein coronas form surfaces in physiological fluids, noncovalent...
Silica-coated nanoparticles are widely used in biomedical applications such as theranostics, imaging, and drug delivery. While silica-coated biocompatible, experimental evidence shows that they can trigger innate immune reactions, a broader understanding of what types reactions caused how to mitigate them is needed. Herein, we investigated the noncovalent surface functionalization silica with purified proteins inhibit nanoparticle-induced complement activation macrophage uptake, two most...
We designed and fabricated large arrays of polymer pens having sub-20 nm tips to perform chemical lift-off lithography (CLL). As such, we developed a hybrid patterning strategy called polymer-pen (PPCLL). demonstrated PPCLL using pyramidal v-shaped arrays. Associated simulations revealed nanometer-scale quadratic relationship between contact line widths the two other variables: base vertical compression distances. devised stamp support system consisting interspersed flat-tipped that are...
We introduce a graphene-based nanofluidic cell that facilitates in situ imaging of liquid samples via transmission electron microscopy. The combines the benefits graphene cells—namely, high resolution, reduced charging effects, and excellent sample stability—with ability to reactants control fluid concentrations as provided by conventional silicon-nitride-windowed flow cells. offers significantly less window bowing compared existing commercial holders. demonstrate performance gold...
Nanoribbon- and nanowire-based field-effect transistors (FETs) have attracted significant attention due to their high surface-to-volume ratios, which make them effective as chemical biological sensors. However, the conventional nanofabrication of these devices is challenging costly, posing a major barrier widespread use. We report high-throughput approach for producing arrays ultrathin (∼3 nm) In2O3 nanoribbon FETs at wafer scale. Uniform films semiconducting were prepared on Si/SiO2...
Chemical lift-off lithography (CLL) is a subtractive soft-lithographic technique that uses polydimethylsiloxane (PDMS) stamps to pattern self-assembled monolayers of functional molecules for applications ranging from biomolecule patterning transistor fabrication. A hallmark CLL preferential cleavage Au–Au bonds, as opposed bonds connecting the molecular layer substrate, i.e., Au–S bonds. Herein, we show can be used more broadly variety substrates composed coinage metals (Pt, Pd, Ag, Cu),...
Innovative technologies for intracellular delivery are ushering in a new era gene editing, enabling the utilization of patient's own cells stem cell and immunotherapies. In particular, cell-squeezing platforms provide unconventional forms delivery, deforming through microfluidic constrictions to generate transient pores enable effective diffusion biomolecular cargo. While these devices promising gene-editing platforms, they require frequent maintenance due accumulation cellular debris,...
Supported lipid membranes are versatile biomimetic coatings for the chemical functionalization of inorganic surfaces. Developing simple and effective fabrication strategies to form supported with micropatterned geometries is a long-standing challenge. Herein, we demonstrate how combination lift-off lithography (CLL) easily prepared bicelle nanostructures can yield micropatterned, on gold surfaces high pattern resolution, conformal character, biofunctionality. Using CLL, functionalized...
We demonstrate a method for the selective encapsulation of carbon nanotubes in thin parylene films using iron as sacrificial lift‐off layer. The serves an inhibitor deposition and prevents molecules from linking, thus facilitating area coating after lift‐off.