A5017638910- Zebrafish Biomedical Research Applications
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Glioma Diagnosis and Treatment
- Cancer-related gene regulation
- Pancreatic function and diabetes
- Neuroblastoma Research and Treatments
- Neonatal Respiratory Health Research
- Cancer, Hypoxia, and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Stress Responses and Cortisol
- Acute Myeloid Leukemia Research
- Adipose Tissue and Metabolism
- Nanomaterials and Printing Technologies
- CAR-T cell therapy research
- Protein Degradation and Inhibitors
- Gold and Silver Nanoparticles Synthesis and Applications
- Immune responses and vaccinations
- Innovative Microfluidic and Catalytic Techniques Innovation
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Brain Metastases and Treatment
- Microfluidic and Bio-sensing Technologies
University of California, Los Angeles
2017-2023
California NanoSystems Institute
2020
Beth Israel Deaconess Medical Center
2014-2017
Harvard University
2014-2017
Beth Israel Deaconess Hospital
2014
Identifying signaling pathways that regulate hematopoietic stem and progenitor cell (HSPC) formation in the embryo will guide efforts to produce expand HSPCs ex vivo. Here we show sterile tonic inflammatory regulates embryonic HSPC formation. Expression profiling of progenitors with lymphoid potential cells (HSCs) from aorta/gonad/mesonephros (AGM) regions midgestation mouse embryos revealed a robust innate immune/inflammatory signature. Mouse lacking interferon γ (IFN-γ) or IFN-α zebrafish...
Vitamin D insufficiency is a worldwide epidemic affecting billions of individuals, including pregnant women and children. Despite its high incidence, the impact active vitamin D3 (1,25(OH)D3) on embryonic development beyond osteo-regulation remains largely undefined. Here, we demonstrate that 1,25(OH)D3 availability modulates zebrafish hematopoietic stem progenitor cell (HSPC) production. Loss Cyp27b1-mediated biosynthesis or receptor (VDR) function by gene knockdown resulted in...
We developed an unconventional seed-mediated in situ synthetic method, whereby gold nanostars are formed directly on the internal walls of microfluidic reactors. The dense plasmonic substrate coatings were grown channels with different geometries to elucidate impacts flow rate and profile reagent consumption, product morphology, density. Nanostar growth was found occur flow-limited regime our results highlight possibility creating shape gradients or incorporating multiple morphologies same...
Innovative technologies for intracellular delivery are ushering in a new era gene editing, enabling the utilization of patient's own cells stem cell and immunotherapies. In particular, cell-squeezing platforms provide unconventional forms delivery, deforming through microfluidic constrictions to generate transient pores enable effective diffusion biomolecular cargo. While these devices promising gene-editing platforms, they require frequent maintenance due accumulation cellular debris,...
The core binding factor (CBF) gene RUNX1 is a target of chromosomal translocations in leukemia, including t(8;21) acute myeloid leukemia (AML). Normal CBF function essential for activity AML1-ETO, product the t(8;21), and survival several leukemias lacking mutations. Using virtual screening optimization, we developed Runt domain inhibitors which bind to disrupt its interaction with CBFβ. On-target was demonstrated by inhibitors' ability depress hematopoietic cell formation zebrafish embryos,...
Intracellular delivery technologies that are cost-effective, non-cytotoxic, efficient, and cargo-agnostic needed to enable the manufacturing of cell-based therapies as well gene manipulation for research applications. Current capable delivering large cargoes, such plasmids CRISPR-Cas9 ribonucleoproteins (RNPs), plagued with high costs and/or cytotoxicity often require substantial specialized equipment reagents, which may not be available in resource-limited settings. Here, we report an...