A5019103330- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Metal Extraction and Bioleaching
- Probiotics and Fermented Foods
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Diet, Metabolism, and Disease
- Phytase and its Applications
- Magnetic and Electromagnetic Effects
- Microbial Natural Products and Biosynthesis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Extraction and Separation Processes
- Minerals Flotation and Separation Techniques
- Enzyme Catalysis and Immobilization
- Catalysis and Hydrodesulfurization Studies
- Tryptophan and brain disorders
- Microbial Metabolic Engineering and Bioproduction
- Animal Nutrition and Physiology
- Biofuel production and bioconversion
- Marine Sponges and Natural Products
- Coal and Its By-products
- Advanced biosensing and bioanalysis techniques
- Proteins in Food Systems
- Recycling and Waste Management Techniques
- Plant-Microbe Interactions and Immunity
Pakistan Institute of Engineering and Applied Sciences
2020-2025
National Institute for Biotechnology and Genetic Engineering
2008-2025
Cairo University
2024
National Institute of Genetic Engineering and Biotechnology
2022
University of Groningen
2008-2012
Molecular Research Institute
2011
University of Karachi
2001
Microorganisms synthesize diverse types of exopolysaccharides (EPSs). EPSs with varying structural and physical properties can demonstrate unique health benefits, which allow for their tailored applications as functional foods such prebiotics. Levan, a fructose-based EPS, is gaining considerable attention an effective prebiotic to support the growth beneficial gut bacteria. Consequently, this enhances digestive health, boosts immune system, reduces risk chronic diseases. Unfortunately,...
Fructansucrase enzymes polymerize the fructose moiety of sucrose into levan or inulin fructans, with β (2-6) and (2-1) linkages, respectively. Here, we report an evaluation fructan synthesis in three Lactobacillus gasseri strains, identification fructansucrase-encoding genes characterization recombinant proteins (oligosaccharide) products. High-performance anion-exchange chromatography nuclear magnetic resonance analysis fructo-oligosaccharides (FOS) polymers produced by L. strains revealed...
ABSTRACT Fructansucrase enzymes polymerize the fructose moiety of sucrose into levan or inulin fructans, with β(2-6) and β(2-1) linkages, respectively. The probiotic bacterium Lactobacillus johnsonii strain NCC 533 possesses a single fructansucrase gene (open reading frame AAS08734) annotated as putative levansucrase precursor. However, 13 C nuclear magnetic resonance (NMR) analysis fructan product synthesized in situ revealed that this is type. ftf L. was cloned expressed to elucidate its...
Surface-enhanced Raman spectroscopy has been employed to analyze the antibacterial potential of an N-heterocyclic carbene selenium complex (NHC Se complex), its ligand, and commercial derivative ornidazole. (SERS) is analytical method that enhances signals through interaction molecules with nanostructured metallic surfaces. Two pathogenic bacteria, including gram positive Staphylococcus aureus (S. aureus) gram-negative Escherichia Coli (E. coli), were used characterize activity ligand. After...
The major challenge in large‐scale industrial use of lipopeptide surfactin is the low yield by indigenous bacterial strains and higher cost its production that have been proved as a limiting factor commercial applications. Therefore, there an urgent demand for high‐yielding can be achieved through strain improvement. A first report on combination UV gamma‐irradiation mutagenesis development hyperproducing mutants Bacillus spp. to significant resulted twofold enhancement yield. mutant was...
Out of 17 samples collected from diverse environments, 110 bacterial isolates varied characteristics were screened for their dibenzothiophene-desulphurizing activity. A single isolate, Eu-32, originating a soil sample taken the roots eucalyptus tree, displayed This isolate metabolized dibenzothiophene to 2-hydroxybiphenyl (2-HBP), as detected by HPLC, and was also able use other organic sulphur compounds sole source. Based on morphological, biochemical molecular studies, it found that...
Culturable bacterial biodiversity and industrial importance of the isolates indigenous to Khewra salt mine, Pakistan was assessed. PCR Amplification 16S rDNA carried out by using universal primers FD1 rP1and products were sequenced commercially. These gene sequences compared with other in GenBank databases find closely related sequences. The alignment these available from database construct a phylogenetic tree for bacteria. genes deposited accession numbers obtained. Most belonged different...
The probiotic bacterium Lactobacillus reuteri 121 produces two fructosyltransferase enzymes, a levansucrase and an inulosucrase. Although these enzymes share high sequence similarity, they differ significantly in the type size distribution of fructooligosaccharide products synthesized from sucrose, their activity levels. In order to examine contribution specific amino acids such differences, 15 single four multiple inulosucrase mutants were designed that affected residues are conserved but...