A5101753603- HER2/EGFR in Cancer Research
- Silk-based biomaterials and applications
- Biochemical and Structural Characterization
- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Research Studies
- Neurobiology and Insect Physiology Research
- Medical Imaging Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Regulation of Appetite and Obesity
- Peptidase Inhibition and Analysis
- Sepsis Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Biochemical Analysis and Sensing Techniques
- Invertebrate Immune Response Mechanisms
- Silkworms and Sericulture Research
- Immune Response and Inflammation
- Genomics, phytochemicals, and oxidative stress
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Molecular Biology Techniques and Applications
- Pancreatic function and diabetes
- Enzyme Production and Characterization
- Bacterial biofilms and quorum sensing
- Adipose Tissue and Metabolism
Anhui Science and Technology University
2025
Anhui University of Science and Technology
2025
Heilongjiang Bayi Agricultural University
2025
China Agricultural University
2019-2024
Ningxia Medical University
2009-2023
National University of Singapore
2006-2021
Hubei University of Medicine
2015-2021
Zhejiang University
2003-2020
Northwestern Polytechnical University
2002-2019
Sir Run Run Shaw Hospital
2003-2006
Spider silks are renowned for their excellent mechanical properties and biomimetic industrial potentials. They formed from the natural refolding of water-soluble fibroins with alpha-helical random coil structures in silk glands into insoluble fibers mainly beta-structures. The at atomic resolution formation mechanism remain largely unknown. Here, we report 3D individual domains a approximately 366-kDa eggcase protein that consists 20 identical type 1 repetitive domains, one 2 domain,...
Death signaling provided by tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) can induce death in cancer cells with little cytotoxicity to normal cells; this cell has been thought involve caspase-dependent apoptosis. Reactive oxygen species (ROS) are also mediators that death, but their roles TRAIL-induced apoptosis have not elucidated fully. In the current study, we investigated ROS and caspases human pancreatic undergoing two different types of necroptosis. TRAIL...
Biodegradable bone materials can degrade gradually in response to physiological environment and simultaneously promote regeneration, thus avoiding the demand of secondary surgery. With continuous degradation, environmental pH around could be significantly changed. In current work, effects on macrophage polarization osteoimmunomodulation were investigated. The results showed that viability macrophages was inhibited when decreased from 7.4 6.2 while remained unchanged increased 8.6. morphology...
Maize seedlings in cold regions and high latitude often face abiotic stress. As a result, weak affect maize production, The commonly used seed coating agents production are mainly to prevent biological stress of pests diseases, have little effect on seedling vigor resistance. In this experiment, the combination graphene oxide (GO) agent can effectively diseases increase growth seedlings.The effects different concentrations composite GO photosynthetic carbon nitrogen metabolism activity were...
Maize is a critical crop for food, feed, and bioenergy worldwide. This study characterized the genetic diversity population structure of 212 important inbred lines collected from Southeast China breeding program using Maize6H-60K single nucleotide polymorphism (SNP) array. To investigate architecture plant height (PH) ear (EH), genome-wide association analysis (GWAS) was performed on this in 2021 2022. Cluster grouped maize into 10 distinct categories. GWAS identified significant...
Abstract Oxidative stress has been implicated as a major mechanism underlying the pathogenesis of neurodegenerative disorders. ROS (reactive oxygen species) can cause cell death via apoptosis. NGF (nerve growth factor) differentiated rat PC12 cells have extensively used to study differentiation and apoptosis neurons. This investigated protective effects puerarin in H 2 O ‐induced cells, possible molecular mechanisms involved. Differentiated were incubated with 700 μM absence or presence...
Spider silk is protein fibers with extraordinary mechanical properties. Up to now, it still poorly understood how proteins are kept in a soluble form before spinning into and the molecules aligned orderly fibers. Minor ampullate spidroin one of seven types proteins, which consists four domains: N-terminal domain, C-terminal domain (CTD), repetitive (RP) linker (LK). Here we report tertiary structure CTD secondary structures RP LK aqueous solution, their roles stability, solubility fiber...
Abstract The hepatopancreatic duct (HPD) system links the liver and pancreas to intestinal tube is composed of extrahepatic biliary duct, gallbladder, pancreatic duct. Haematopoietically expressed-homeobox (Hhex) protein plays an essential role in establishment HPD; however, molecular mechanism remains elusive. Here, we show that zebrafish hhex-null mutants fail develop HPD characterized by lacking marker Annexin A4 sox9b. hepatobiliary part mutant replaced intrahepatic expressing fatty...
Heat shock protein (HSP)60 is primarily a mitochondrial protein. Previous experiments have found that changes in the location of intracellular HSP60 been associated with apoptosis. Extracellular mediates apoptosis via its ligand, Toll‑like receptor (TLR)‑4. TLR‑4 an important factor expressed on microglia, central role generating neuroimmune responses pathogenesis neurodegenerative disorders. Naloxone highly effective nonselective opioid antagonist, and has reported to be pharmacologically...
Liver cancer is one of the leading causes death worldwide. Although radiotherapy and chemotherapy are effective in general, they present various side effects, significantly limiting curative effect. Increasing evidence has shown that dietary intake phytochemicals plays an essential role chemoprevention or tumors. In this work, HepG2 cells nude mice with HepG2-derived xenografts were treated grape seed proanthocyanidins (GSPs). The results showed GSPs induced autophagy, inhibition autophagy...
Hepatocellular carcinoma (HCC) is one of the common malignancies leading to death. Although radiotherapy and chemotherapy have certain effects, their side effects limit therapeutic effect. Phytochemicals recently been given more attention as promising resources for cancer chemoprevention or due safety. In this study, grape seed proanthocyanidins (GSPs) on apoptosis, cell cycle, mitogen-activated protein kinase (MAPK) pathway-related proteins non-steroidal anti-inflammatory drug-activated...
Significance Our manuscript is focused on the structural and functional role of minor silk component TuSp2 in spider eggcase formation. We present structure core domain reveal how this renders main TuSp1 more favorable for molecular chain alignment, which critical strong fiber further designed a series biomimetic minispidroin complexes showed that artificial can surpass its native counterpart strength significantly. This study represents distinct advance understanding The strategy developed...
Glycyrrhiza uralensis polysaccharides (GUPS) are widely applied in biomedicine and functional food due to their multiple pharmacological activities low toxicity. Despite widespread use, the vivo metabolic profile of GUPS remains poorly understood. To address this gap, we developed a quantitative analysis method that involves labeling with visible fluorescein (5-DTAF) near-infrared (NIR) (Cy7), resulting stable conjugates substitution degrees 0.81% for 5-DTAF 0.39% Cy7. The pharmacokinetic...
C-terminal domains (CTDs) of various spidroins are relatively conserved in the amino acid sequence and have been suggested to perform similar functions. Here, we solved structure CTD an aciniform spidroin using NMR spectroscopy with a two-point mutant studied its functional role several constructs. The CTDs aciniform, major, minor ampullate adopt same domain-swapping dimeric folding although their identities 24-40%. Unlike major spidroins, had no obvious effects on preventing from...
Mulberry extract has been proven to have the effect of resisting alcohol damage, but its mechanism is still unclear. In this study, composition mulberry ethanol (MBE) was identified by LC-MS/MS and main components MBE were ascertained measuring. Gastric mucosal epithelial (GES-1) cells used elucidate rutin (the central part MBE) helped protect against damage. The results revealed that phenolics accounted for majority MBE, accounting 308.6 mg/g gallic acid equivalents 108 substances...
Objective: To observe the difference of androgen and inflammatory cytokines level in atherosclerosis analyse their relations.Method: Both carotid arteries lower extremity were subjected to ultrasonic examination by Doppler's method.Those with much atheromatous plaque formation ranged into case group, those normal result formed control group.Total, free testosterone estradiol assayed radioimmunoassay.C reactive protein (CRP) was nepheloturbidity.Tumor necrosis factor-α (TNF-α), Interleukin-6...