Aim: To elucidate dynamics and functions in colonic macrophage subsets, their regulation by Bifidobacterium breve (B.breve) its associated metabolites the initiation of colitis-associated colorectal cancer (CAC).Methods: Azoxymethane (AOM) dextran sodium sulfate (DSS) were used to create a CAC model.The tumor-suppressive effect B. variations subsets evaluated.Intestinal macrophages ablated determine role protective effects breve.Efficacious molecules produced identified non-targeted targeted...

Oral squamous cell carcinoma (OSCC) has the characteristics of early regional lymph node metastasis. OSCC patients often have poor prognoses and low survival rates due to cervical metastases. Therefore, it is necessary rely on a reasonable screening method quickly judge metastastic condition develop appropriate treatment plans. In this study, widely used pathological sections with hematoxylin-eosin (H&E) staining are taken as target, combined advantages hyperspectral imaging technology,...

Biodegradable bone materials can degrade gradually in response to physiological environment and simultaneously promote regeneration, thus avoiding the demand of secondary surgery. With continuous degradation, environmental pH around could be significantly changed. In current work, effects on macrophage polarization osteoimmunomodulation were investigated. The results showed that viability macrophages was inhibited when decreased from 7.4 6.2 while remained unchanged increased 8.6. morphology...

Exopolysaccharide (EPS) from probiotic bacteria received more attention these days for its bioactivities on immune system modulation, intestinal microbiota antioxidants and anticarciogenic effect. Yet, are of interspecies diversity, thus examining the gene clusters EPS biosynthesis pathways is essential selecting better application specific EPS. In this study, we isolated a new Bifidobacterium strain, named B.breve lw01. A complete genome lw01 was sequenced revealing circular 2,313,172 bp...

Recent advances in immunotherapy, as a part of the multidisciplinary therapy, has gradually gained more attention. However, only small proportion patients who sensitive to therapy could gain benefits. An increasing number studies indicate that intestinal microbiota enhance efficiency cancer immunotherapy. As one main probiotics, Bifidobacterium plays an important role immune regulation, which been proved by animal research and human clinical study. But detailed mechanism was not clearly...

Abstract Background Long-term use of anti-resorptive or anti-angiogenic drugs in cancer patients with odontogenic infections may lead to medication-related osteonecrosis the jaw (MRONJ). This study investigated whether agents aggravate MRONJ occurrence anti-resorptive-treated patients. Methods The clinical stage and jawbone exposure caused by different drug regimens were analyzed ascertain aggravation effect on drug-based MRONJ. Next, a periodontitis mice model was established, tooth...

Tuberculous meningitis (TBM) is the most serious type of tuberculosis infection, and there a lack accurate diagnostic targets for TBM. Therefore, it great clinical public health significance to find specific target early diagnosis prediction prognosis From January 2021 February 2024, 110 TBM patients 122 with non-tumor, non-infectious headaches were admitted Hunan Chest Hospital. To compare two groups, cerebrospinal fluid (CSF) levels sCD163 MMP-9, as well serum NCAM1 protein levels,...

Niclosamide is a traditional anti-tapeworm drug that exhibits potent anti-cancer activity. Our previous study showed niclosamide induces cell cycle arrest in G1 phase. Nevertheless, the underlying mechanism remains unknown. The following investigated molecular through which induced head and neck squamous carcinoma (HNSCC) lines. effect of on human HNSCC line WSU-HN6 CNE-2Z were analyzed using IncuCyte ZOOMTM assay, flow cytometry (FCM), real-time PCR western blot. Luciferase assay was...

As the rate-limit enzyme of pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PD) plays important roles in tumour progression, but exact mechanism through which G6PD controls cancer metastasis remains unclear.G6PD expression resected oral squamous cell carcinoma (OSCC) samples was analysed by immunohistochemistry. The effects and suppression on OSCC lines were measured transwell assay, wound healing western lectin blot, mass spectrometer analysis, ChIP-PCR, luciferase reporter...

Abstract Objective This study evaluated the effectiveness of a submental island flap in closing advanced mandibular medication‐related osteonecrosis jaw (MRONJ) wounds patients with malignant tumors. Subjects and methods A total 85 stage II III MRONJ mandible tumor as their primary disease were retrospectively analyzed. All underwent surgical treatment, soft tissue wound closure was performed either (SIF) or mucoperiosteal (MF). Univariate multifactorial models applied to analyze factors...

Staphylococcus aureus ( S. ) is a typical kind of symbiotic bacteria, which can cause human pneumonia, food poisoning, and other health problems. Nowadays, the corresponding prevention treatment have been hot issue general concern in related research areas. However, mechanism action against not well understood. In order to tackle such problem, we used broth microdilution discuss antibacterial effect 5‐methyl‐2‐isopropylphenol determine inhibitory concentration. addition, membrane potential...

Abstract Periodontitis is a significant independent risk factor for atherosclerosis. Yet, the exact mechanism of action still not fully understood. In this study, we investigated effect exosomes‐miR‐155‐5p derived from periodontal endothelial cells on atherosclerosis in vitro and vivo. Higher expression miR‐155‐5p was detected plasma exosomes patients with chronic periodontitis (CP) carotid (CAS) compared to CP. Also, level associated severity miR‐155‐5p‐enriched HUVECs increased...

Two copper(II) complexes [CuL1Cl]ClO4 and [CuL2MeCN](ClO4)2·H2O were synthesized (L1 = 1-(benzimidazole-2-ylmethyl)-1,4,7-triazacyclononane, L2 1,4-bis(benzimidazole-2-ylmethyl)-1,4,7-triazacyclonone). The benzimidazole groups N-substituents of tacn, the are more stable than their parents. They able to catalyse dismutation superoxide anion in aqueous solutions at physiological pH bovine serum albumin solution (0.5 mg ml−1). X-ray structure analysis EPR electronic spectra show that complex 2...

Aseptic prosthetic loosening (APL) often leads to the failure of prostheses. It is inseparable from wear-particle-induced macrophage-mediated inflammatory responses and osteolysis. Mg2+ a metal ion with excellent anti-inflammatory properties. Herein, was introduced into nanomedicine (MgO nanoparticles (MNPs)) protect against APL. MNPs could be phagocytized by macrophages gradually degraded intracellularly. Following treatment, lipopolysaccharide (LPS)-activated polarized deeper M1 phenotype...

Abstract Objective To explore the role of CD10 in cisplatin resistance oral squamous cell carcinoma (OSCC) and its association with Hedgehog (Hh) signaling pathway cancer stem cells (CSCs). Methods The correlation between viability expression was analyzed different OSCC lines after treatment. Genes related to chemotherapy resistance, epithelial–mesenchymal transition were detected by quantitative real‐time PCR (qPCR) high low cells. Mouse xenograft model venous metastasis used potential...

Abstract Background Cancer stem cells (CSCs) drive tumor initiation and progression participate in chemoresistance. We recently discovered that oral squamous cell carcinoma (OSCC) highly express CD10 (CD10H cells) present cancer (CSC)-associated characteristics, which, turn, affect the growth, epithelial-mesenchymal transition (EMT), resistance to cisplatin. In this study, we further investigated mechanism vitro vivo . hypothesized IL8 might regulate migration, invasion, cisplatin of...

Background: The hepatocellular carcinoma (HCC) is a highly aggressive and common malignancy worldwide. Accumulating evidence has demonstrated pivotal role of long noncoding RNAs (lncRNAs) in various tumors. However, the function intergenic lncRNA LINC00908 still unknown HCC. Methods: RT-qPCR method was used to quantify expression LINC00908. Migration viability assay were performed evaluate vitro effect xenograft tumor model measure vivo effect. Immunoblot identify association with Sox-4...

Objective: Refractory infection is an important factor affecting the progression of medication-related osteonecrosis jaw (MRONJ) from clinical stage I to II/III. The aim this study was explore distribution bacteria and their association with inflammatory pathway II/III MRONJ.Materials Methods: Nine specimens fresh inflammation tissue, located next necrotic bone or sequestrum, were collected MRONJ patients. normal oral mucosa healthy 16S rRNA gene sequencing method used determine...

Human fibroleukin 2 (Fgl2), a member of the fibrinogen superfamily, can cleave prothrombin to generate thrombin or is secreted in soluble form as new type effector Tregs with immunomodulatory functions. However, there little research on role Fgl2 cutaneous squamous cell carcinoma (CSCC) growth. We examined expression samples from CSCC patients and lines. Then, effect was evaluated vitro animals. Regulation autophagy by explored CSCC. Coimmunoprecipitation (Co-IP) immunofluorescence...