Cytomegalovirus (CMV) infection enhances expression of several cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-8, to the benefit virus replication dissemination. However, stimulus for certain cytokine production remains unclear. CMV encodes a series proteins that alter and/or mimic functions leukocyte migration, activation, responses. Our study revealed human (HCMV)-encoded UL128 protein, which...

The majority of experiments show that tumor necrosis factor-alpha (TNF-α) inhibits osteogenic differentiation mesenchymal stem cells and pre-osteoblasts by activated nuclear factor-kappaB (NF-κB) signaling. However, the underlying mechanisms which NF-κB signaling are not fully understood. aim present study was to investigate whether EphB4 inhibition mediates effects TNF-α-activated on pre-osteoblasts.Murine MC3T3-E1 were treated with 10 ng/mL TNF-α. inhibitor, pyrrolidine dithiocarbamate,...

Abstract Objective This study evaluated the effectiveness of a submental island flap in closing advanced mandibular medication‐related osteonecrosis jaw (MRONJ) wounds patients with malignant tumors. Subjects and methods A total 85 stage II III MRONJ mandible tumor as their primary disease were retrospectively analyzed. All underwent surgical treatment, soft tissue wound closure was performed either (SIF) or mucoperiosteal (MF). Univariate multifactorial models applied to analyze factors...

Parathyroid Hormone (PTH) can exert both anabolic and catabolic effects on the skeleton, potentially through expression of PTH type1 receptor (PTH1R), which is highly expressed in osteocytes. To determine cellular molecular mechanisms responsible, we examined osteoblast to osteocyte differentiation using primary osteocytes IDG-SW3 murine cell line, differentiate from osteocyte-like cells vitro express GFP under control dentin matrix 1 (Dmp1) promoter. treatment resulted an increase some...

Bone remodeling is affected by mechanical stimulation. Osteocytes are the primary load-sensing cells in bone, and can regulate osteoblast osteoclast activity, thus playing a key role bone remodeling. Further, mass during exercise also regulated Leukemia inhibitory factor (LIF). This study aimed to investigate of LIF response vivo vitro, elucidate mechanism which osteocytes secrete osteoblasts osteoclasts.A tail-suspension (TS) mouse model was used this mimic muscular disuse. ELISA...

ABSTRACT Specific types of human papillomavirus (HPV) are strongly associated with the development cervical carcinoma. The HPV E6 oncoprotein from degrades p53 and abrogates cell cycle checkpoints. Nonetheless, functional has been observed in cancer. We have previously identified a p53-independent function attenuating postmitotic G 1 -like checkpoint that can lead to polyploidy, an early event during carcinogenesis predisposes cells aneuploidy. How promotes progression presence its target,...

Adenocarcinoma of the pancreas (PAAD) is a cancerous growth that deteriorates rapidly and has poor prognosis. Researchers are investigating autophagy in PAAD to identify new biomarker treatment target. An autophagy-related gene (ARG) model for overall survival (OS) was constructed using multivariate Cox regression analyses. A cohort Cancer Genome Atlas (TCGA)-PAAD used as training group basis construction. This prediction validated with several external datasets. To evaluate performance,...

Objective: Refractory infection is an important factor affecting the progression of medication-related osteonecrosis jaw (MRONJ) from clinical stage I to II/III. The aim this study was explore distribution bacteria and their association with inflammatory pathway II/III MRONJ.Materials Methods: Nine specimens fresh inflammation tissue, located next necrotic bone or sequestrum, were collected MRONJ patients. normal oral mucosa healthy 16S rRNA gene sequencing method used determine...

Summary Background Orthodontic tooth movement (OTM) has previously been considered an inflammatory process. However, recent studies suggest that exosomes may play important role in the cellular microenvironment of OTM. microRNAs (miRNAs) are one major constituents exosomes. This study aims to investigate biological characteristics miRNAs secreted by periodontal ligament stem cells (PDLSCs) due mechanical forces. Materials and methods First, we established a stress model. The PDLSCs were...

Objective To investigate the dynamic expression of SOCS-3, TNF-α in live and spleen mice induced by AOPP, to discuss mechanism MODS so that give some intervention method over future. Method Thirty-six adult BALB/c were divided into 3 groups randomly: AOPP group (n=12). Water normal (n=12) After post poison 2 hours, 6 12 hours 24 hours,the fiver tissue taken out. The SOCS-3 detected RT-PCR. And data analyzed with ANOVA. Results 2,6,12,24 mRNA increased obviously liver compared (P<0.05), it...

Clinical therapeutic and immunoregulatory effects of recombinant SPLUNC1 protein (rSPLUNC1) were evaluated in Mycoplasma ovipneumoniae (Mo)-infected Argali hybrid sheep (AHS). Group A contained six Bashibai (BS) groups B–D AHS each. All manually infected with Mo. Five days post-infection, rSPLUNC1 from BS was injected intratracheally into group C D animals; physiological saline administered to B. Serum IL-5, IL-6, IL-9 quantified by ELISA. After sacrificing the sheep, lung tissues extracted...

C 34 H 26 CdN 4 O 10 S 2 ,triclinic, P1 (no.2), a =9.897(3)Å, b =12.856(6)Å, c =14.076(4) Å, =88.31(3)°, =80.54(2)°, g =69.54(4)°,V =1654.4Å 3 , Z =2, R gt(F) =0.045, wRref(F ) =0.151, T =298 K. Source of materialAm ixture disodium 9,10-dioxoanthracene-2,6,-disulfonate (Na 22,6-ADS, 0.1 mmol, 0.042 g), Cd(NO3)2 • 4H2O(0.1 0.031 2,2'-bipyridine (2,2'-bipy, 0.016 g) and water mL was stirred for about 30 min, then sealed in aTeflonlined stainless steel autoclave heated at 393 Kfor 3days.The...

This study aimed to analyze the clinical application effect of double-layer soft tissue closure technique (DLST) based on pedicled buccal fat pad in repairing maxillary defects after medication-related osteonecrosis jaw (MRONJ) surgery.

Objective: To investigate the arthroscopic temporomandibular joint disc reduction on outcome of orthodontic patients with anterior displacement without reduction. Methods: From January 2012 to December 2021, forty treated (unilateral/bilateral) and no obvious articular cartilage absorption were selected from Department Orthodontics, Shanghai Ninth People's Hospital, Jiao Tong University School Medicine. The (17.5±3.8) years old (12-25 old), including 8 males 32 females. Twenty who had...

C 46 H 54 Co 3 N 6 O 26 S 2 ,monoclinic, C12/c1(no.15), a =19.411(4)Å, b =9.037(2) Å, c =31.626(7) =96.442(3)°,V =5512.7 Å , Z =4,Rgt(F) =0.076, wR ref(F ) =0.248, T =298 K. Source of materialAm ixture 5-sulfoisophthalic acid monosodium salt (SIP, 0.027 g, 0.1 mmol), Co(CH COO) (0.018 2,2'bipyridine (2,2'-bipy, 0.016 mmol) and 2O( 10 mL) was stirred for about 30 min.The resulting solution sealed in Teflon-lined stainless autoclave heated to 393 Kfor 3days.The bottle cooled ambient...