A5101498006- Immunotherapy and Immune Responses
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- HIV Research and Treatment
- Drug Transport and Resistance Mechanisms
- Ferroptosis and cancer prognosis
- ATP Synthase and ATPases Research
- Immune Cell Function and Interaction
- Nerve injury and regeneration
- Plant biochemistry and biosynthesis
- Phytochemistry and Biological Activities
- Spinal Cord Injury Research
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- interferon and immune responses
- Lymphoma Diagnosis and Treatment
- Research on Leishmaniasis Studies
- vaccines and immunoinformatics approaches
- CRISPR and Genetic Engineering
- Toxic Organic Pollutants Impact
- Extracellular vesicles in disease
- MicroRNA in disease regulation
Shenzhen University
2020-2025
Northeast Agricultural University
2022-2025
Shenzhen University Health Science Center
2019-2022
Xiamen University
2022
Tianjin Medical University Cancer Institute and Hospital
2007-2021
Gansu Agricultural University
2021
China Medical University
2021
Zhejiang A & F University
2019-2020
Shihezi University
2020
Key Laboratory of Guangdong Province
2020
The development of an HIV-1 vaccine that is capable inducing effective and broadly cross-reactive humoral cellular immune responses remains a challenging task because the extensive diversity HIV-1, difference virus subtypes (clades) in different geographical regions, polymorphism human leukocyte antigens (HLA). We performed silico design 3 DNA vaccines, designated pJW4303-MEG1, pJW4303-MEG2 pJW4303-MEG3, encoding multi-epitopes are highly conserved within most prevalent China can be...
Cordycepin, isolated from the traditional medicinal fungus Cordyceps militaris, has gained much attention due to its various clinical functions. Previous reports of L-alanine addition could significantly improve cordycepin production, but molecular mechanism remains unknown. In this study, transcriptome analysis C. militaris with doubled production induced by provides an insight into flexibility network. The biopathways energy generation and amino acid conversion were activated so that...
Phenotypically unstable Schwann cell-like cells (SCLCs), derived from mesenchymal stem (MSCs) require intercellular contact-mediated cues for cell (SCs)-fate commitment. Although rat dorsal root ganglion (DRG) neurons provide signals the conversion of SCLCs into fate-committed SCs, use animal is clinically unacceptable. To overcome this problem, we previously acquired human induced pluripotent cell-derived sensory (hiPSC-dSNs) as surrogates DRG that committed bone marrow to SC fate. In...
The seeds of Torreya grandis (Cephalotaxaceae) are rich in tocopherols, which essential components the human diet as a result their function scavenging reactive oxygen and free radicals. Different T. cultivars (10 selected this study were researched, information is shown Table S1 Supporting Information) vary enormously tocopherol contents (0.28-11.98 mg/100 g). However, little known about molecular basis regulatory mechanisms biosynthesis kernels. Here, we applied single-molecule real-time...
Renal cell carcinoma (RCC), as one of the primary urological malignant neoplasms, shows poor survival, and leading pathological type RCC is clear (ccRCC). Differing from other deaths (such apoptosis, necroptosis, pyroptosis, autophagy), ferroptosis characterized by iron-dependence, polyunsaturated fatty acid oxidization, lipid peroxide accumulation. We analyzed database (FerrDb V2), Gene Expression Omnibus database, The Cancer Genome Atlas ArrayExpress database. Nine genes that were...
The emergence of multidrug resistance is always associated with more rapid tumor recurrence and metastasis. P-glycoprotein (P-gp), which a well-known multidrug-efflux transporter, confers enhanced invasion ability in drug-resistant cells. Previous studies have shown that P-gp probably exerts its tumor-promoting function via protein-protein interaction. These interactions were implicated the activation intracellular signal transduction. We previously showed binds to Anxa2 promotes...
Abstract In this study, we aimed to investigate whether and how Golgi phosphoprotein 3 (GOLPH3) facilitates colon cancer metastasis via the regulation of autophagy epithelial–mesenchymal transition (EMT). The role GOLPH3 plays in was analyzed using western blotting, immunohistochemistry, transwell, wound-healing, zebrafish assays. Autophagy EMT were assessed RNA-sequencing (RNA-seq) analysis, mRFP-GFP-LC3 reporter assays, their related markers. Significant associations found between clinical...
It has been observed that bone marrow-derived mesenchymal stem cells (MSCs) migrate towards the injured spinal cord and promote functional recovery when systemically transplanted into traumatized cord. However, mechanisms underlying their migration to remain poorly understood. In this study, we GFP- luciferase-expressing MSCs rat models of injury examined role stromal cell-derived factor 1 (SDF-1)/CXCR4 axis in regulating After intravenous injection, migrated where expression SDF-1 was...
Background. Hepatocellular carcinoma (HCC) is aggressive cancer with a poor prognosis. It has been suggested that the aberrant expression of LOXL2 associated development HCC, but exact mechanism remains unclear. This research aimed at examining level and prognostic value in hepatocellular its relationship immune infiltration predicting upstream noncoding RNAs (ncRNAs). Method. The transcriptome data HCC was first downloaded from Cancer Genome Atlas (TCGA) database to investigate prognosis...
The rapid occurrence of emerging infectious diseases demonstrates an urgent need for a new preclinical experimental model that reliably replicates human immune responses. Here, homozygous humanized leukocyte antigen (HLA)-A11/DR1 transgenic mouse (HLA-A11+/+/DR01+/+/H-2-β2m−/−/IAβ−/−) was generated by crossing HLA-A11 (Tg) mice with HLA-A2+/+/DR01+/+/H-2-β2m−/−/IAβ−/− mice. HLA-A11-restricted response this then examined. Tg expressing chimeric major histocompatibility complex (MHC) molecule...
As a crucial step for human reproduction, embryo implantation is low-efficiency process. Despite rapid advances in recent years, the molecular mechanism underlying remains poorly understood. Here, we used mouse as an animal model and generated single-cell transcriptomic atlas of sites. By analyzing inter-implantation sites uterus control, were able to identify global gene expression changes associated with each cell type. Additionally, predicted signaling interactions between uterine luminal...
Tetracycline (Tet)-regulated expression system has become a widely applied tool to control gene activity. This study aimed improve the Tet-on with superior regulatory characteristics.By comprehensively comparing factors of transactivators, Tet-responsive elements (TREs), orientations induced cassette, and promoters controlling transactivator, we developed an optimal enhanced inducible efficiency lower leakiness. With system, successfully performed effective reversible microRNA, presented...
Transient receptor potential (TRP) channels are critical for insects to detect environmental stimuli and regulate homeostasis. Moreover, this superfamily has become molecular targets insecticides or repellents. Pieris rapae is one of the most common widely spread pests Brassicaceae plants. Therefore, it necessary study TRP (TRPs) in P. rapae. In study, we identified 14 TRPs rapae, including two Water witch (Wtrw) genes. By contrast, only Wtrw gene exists Drosophila functions hygrosensation....
The Bashbay sheep (Ovis aries), an indigenous breed of Xinjiang, China, has many excellent characteristics. It is resistant to Mycoplasma ovipneumoniae infection, the causative agent mycoplasma ovipneumonia, a chronic respiratory disease that harmful industry. To date, knowledge regarding mechanisms responsible for M. pathogenesis in scant. Herein, we report results transcriptome profiling lung tissues from experimentally infected with strain at 4 and 14 days post-infection, comparison...
The effective protective HIV vaccine should elicit either antibodies or T cell response, both. To improve the efficacy of HIV-1 vaccines, HLA polymorphism and diversity are 2 key factors to be considered for development. In this study, we expressed a recombinant multi-epitope protein MEP1 which has same amino acid sequence as DNA Chinese population in our previous report. We found that alone could moderate levels humoral cellular immune responses, but these responses not provide protection...
An experiment was conducted to reveal why the Argali hybrid sheep are susceptible Mycoplasma ovipneumoniae infection, causative agent of mycoplasma ovipneumonia, a chronic respiratory disease that is harmful industry. After nine sheep, divided into three groups, were experimentally infected with an M. strain at 0, 4 and 14 days, transcriptome profiling lung tissues performed by deep RNA sequencing, using Illumina platform. Analysis differentially expressed genes determine concomitant...