A5025585267- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Monoclonal and Polyclonal Antibodies Research
- Periodontal Regeneration and Treatments
- COVID-19 Clinical Research Studies
- Mesenchymal stem cell research
- Protease and Inhibitor Mechanisms
- Bone Tissue Engineering Materials
- Fungal Infections and Studies
- Immunotherapy and Immune Responses
- Osteoarthritis Treatment and Mechanisms
- Peptidase Inhibition and Analysis
- Long-Term Effects of COVID-19
- Protein purification and stability
- Mercury impact and mitigation studies
- COVID-19 diagnosis using AI
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Image Analysis Techniques
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Silk-based biomaterials and applications
- Reconstructive Surgery and Microvascular Techniques
- Medical and Biological Sciences
- Heavy Metal Exposure and Toxicity
- Tracheal and airway disorders
Universidade Estadual Paulista (Unesp)
2013-2025
Rostov State Medical University
2024
Ministry of Health of the Russian Federation
2024
Centro Universitário de Araraquara
2017-2023
High-grade prostate cancers express high levels of matrix metalloproteinases (MMPs), major enzymes involved in tumor invasion and metastasis. However, the cell lines commonly employed for cancer research only small amounts MMPs when cultivated as monolayer cultures, common culture media. The present study was conducted to ascertain whether conditions that include fibronectin can alter MMP2 MMP9 expression by human prostatic epithelial RWPE-1, LNCaP PC-3. These cells were individually seeded...
Introduction The use of the 5-alpha reductase inhibitors (5-ARIs) finasteride and dutasteride for prostate cancer prevention is still under debate. FDA recently concluded that increased prevalence high-grade tumors among 5-ARI-treated patients must not be neglected, they decided to disallow 5-ARIs prevention. This study was conducted verify effects on cell migration invasion related enzymes/proteins in normal human tumoral prostatic lines. Materials Methods RWPE-1, LNCaP, PC3 DU145 cells...
Articular lesions are still a major challenge in orthopedics because of cartilage's poor healing properties. A improvement therapeutics was the development autologous chondrocytes implantation (ACI), biotechnology-derived technique that delivers healthy after vitro expansion. To obtain cartilage-like tissue, 3D scaffolds essential to maintain chondrocyte differentiated status. Currently, bioactive promising as they can deliver growth factors, cytokines, and hormones cells, giving them boost...
Summary Coffee intake has been associated with a low risk of developing cancer, including prostate which is one the most commonly diagnosed cancer in men. However, few studies have evaluated chronic effects caffeine, abundant methylxanthine coffee, on morphology and physiology. In present study, we investigated chronic, low‐dose caffeine rat from puberty to adulthood. Five‐week‐old male Wistar rats were randomized into two experimental groups: caffeine‐treated (20 ppm drinking water, n = 12)...
Clinical experience for peripheral arterial disease treatment shows poor results when synthetic grafts are used to approach infrapopliteal segments. However, tissue engineering may be an option yield surrogate biocompatible neovessels. Thus, biological decellularized scaffolds could provide natural architecture use in engineering, the absence of ideal autologous veins reduces surgical options. The goal this study was evaluate different chemical induced decellularization protocols inferior...
Matrix metalloproteinases (MMPs) are zinc (Zn(2+)) and calcium (Ca(2+)) dependant endopeptidases, capable of degradation numerous components the extracellular matrix. Cadmium (Cd(2+)) is a well known environmental contaminant which could impair activity MMPs. In this sense, study was conducted to evaluate if Cd(2+) intake inhibits these endopeptidases activities at rat prostate testicles it directly MMP2 MMP9 gelatinolytic assays when present in incubation buffer. To investigate hypothesis,...
BackgroundCardiovascular diseases remain leaders as the major causes of mortality in Western society. Restoration circulation through construction bypass surgical treatment is regarded gold standard peripheral vascular diseases, and grafts are necessary for this purpose. The great saphenous vein often not available synthetic have their limitations. Therefore, new techniques to produce alternative been developed and, sense, tissue engineering a promising provide biocompatible grafts. This...
Mesenchymal stem cells derived from the synovial membrane (MSCSM) have a greater potential for joint regeneration, besides capacity chondrogenic differentiation, since they are source closer to chondrocytes. This study aimed cultivate and evaluate viability differentiation of MSCSM encapsulated in three-dimensional alginate hydrogel (HA) scaffold. Samples metatarsophalangeal 4 horses were collected by astroscopic surgery. These subjected enzymatic digestion, isolated mesenchymal cells,...
The purpose of this study was to evaluate the effect intralesional Mesenchymal Stem Cells (MSC) on treatment experimentally induced articular chondral defects in horses, emphasizing benefits application veterinary medicine. Chondral were medial femoral trochlea both hind limbs four horses. Thirty days post induction; horses divided into two groups. G1 submitted with MSC and G2 control group. Clinical evaluations, synovial fluid analysis Prostaglandin E2 (PGE2) assessment performed prior...
A three-dimensional (3D) lung aggregate model based on sodium alginate scaffolds was developed to study the interactions between Paracoccidioides brasiliensis (Pb) and epithelial cells. The suitability of 3D as an infection examined using cell viability (cytotoxicity), metabolic activity, proliferation assays. Several studies exemplify similarity cultures living organisms, which can generate complementary data due greater complexity observed in these designed models, compared 2D cultures....
OBJETIVOS: O presente estudo teve como objetivo cultivar condrócitos retirados da articulação do joelho de coelhos encapsulados em hidrogel alginato (HA) e caracterizar a produção matriz extracelular (ECM). MÉTODOS: A cartilagem articular foi removida coelhos, com três seis meses, fragmentada pedaços 1mm submetida à digestão enzimática. Uma concentração 1x106 céls/mL foram ressuspensas uma solução sódio 1,5% (w/v), seguida fez-se o processo gelatinização CaCl2 (102 mM), permitindo formação...
To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.This study was performed New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS Triton X-100 detergents), enzymatic methods (DNase RNase).Generally, cells surrounding soft tissues successfully removed, but none protocol removed from cartilage. However, longer more...
The interaction between bacteriophages and integrins has been reported in different cancer cell lines, efforts have undertaken to understand these interactions tumor cells along with their possible role gene alterations, the aim develop new therapies. Here, we report that non-specific of T4 M13 human PC-3 results differential migration varied expression integrins. (at 70% confluence) were exposed 1 × 107 pfu/mL either lytic bacteriophage or filamentous bacteriophage. After 24 h exposure,...
The prevalence of chronic venous disease is high and occurs more frequently in females. According to the clinical, etiological, anatomical, pathological classification (CEAP) definition, reticular veins are included C1 class mainly associated with aesthetic complaints. Several invasive techniques used for treatment, including mini phlebectomy, laser ablation, radiofrequency ablation. However, a wide range sclerosing agents may serve as minimally alternatives, promoting chemical sclerosis...