A5002136835- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
- Glioma Diagnosis and Treatment
- Cancer Research and Treatments
- PARP inhibition in cancer therapy
- Sphingolipid Metabolism and Signaling
- DNA Repair Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Brain Metastases and Treatment
- Autophagy in Disease and Therapy
- Genetics and Neurodevelopmental Disorders
- Nanoparticle-Based Drug Delivery
- Peptidase Inhibition and Analysis
- Management of metastatic bone disease
- Adenosine and Purinergic Signaling
- Coenzyme Q10 studies and effects
- Advanced Electron Microscopy Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Global Health and Surgery
- Sirtuins and Resveratrol in Medicine
- Monoclonal and Polyclonal Antibodies Research
- RNA regulation and disease
- Migraine and Headache Studies
- Medical Imaging and Analysis
- Transcranial Magnetic Stimulation Studies
Washington University in St. Louis
2008-2023
Stanford University
2018-2023
Stanford Medicine
2019-2023
Neurological Surgery
2016
Norwegian Cancer Society
2016
University of Washington
2013
Mallinckrodt (United States)
2012
Molecular Oncology (United States)
2008
Nucleophosmin (NPM) (B23) is an essential protein in mouse development and cell growth; however, it has been assigned numerous roles very diverse cellular processes.Here, we present a unified mechanism for NPM's role NPM directs the nuclear export of both 40S 60S ribosomal subunits.NPM interacts with rRNA large small subunit proteins also colocalizes nucleolus, nucleus, cytoplasm.The transduction shuttlingdefective mutants or loss Npm1 inhibited subunits, reduced available pool cytoplasmic...
Accumulating evidence suggests cancer cells exhibit a dependency on metabolic pathways regulated by nicotinamide adenine dinucleotide (NAD+). Nevertheless, how the regulation of this cofactor interfaces with signal transduction networks remains poorly understood in glioblastoma. Here, we report phosphoribosyltransferase (NAMPT), rate-limiting step NAD+ synthesis, is highly expressed glioblastoma tumors and patient-derived stem-like (GSCs). High NAMPT expression correlates decreased patient...
// David Y.A. Dadey 1,5 , Vaishali Kapoor 1 Arpine Khudanyan Fumihiko Urano 2,3 Albert H. Kim 4,7,8 Dinesh Thotala 1,7 and Dennis E. Hallahan 1,6,7,8 Department of Radiation Oncology, Washington University School Medicine, St. Louis, MO, USA 2 3 Pathology, 4 Neurological Surgery, 5 Medical Scientist Training Program, 6 Mallinckrodt Institute Radiology, 7 Siteman Cancer Center, 8 Hope Correspondence to: Hallahan, email: Keywords : ATF6, radioresistance, glioblastoma, ER-stress, GRP78 Received...
Purpose: Non-small cell lung cancer (NSCLC) and glioblastoma multiforme (GBM) have poor median survival. NSCLC GBM overexpress glucose regulated protein 78 (GRP78), which has a role in radioresistance recurrence. In this study, we determined the effect of anti-GRP78 antibody combined with ionizing radiation (XRT) on lines both vitro vivoExperimental Design: were treated antibodies evaluated for proliferation, colony formation, death, PI3K/Akt/mTOR signaling. The efficacy tumor growth...
Purpose: Glioblastoma multiforme (GBM) is an aggressive primary brain tumor that radio-resistant and recurs despite surgery, chemo radiotherapy. Autotaxin (ATX) over expressed in various cancers including GBM implicated progression, invasion, angiogenesis. Using the ATX specific inhibitor, PF-8380, we studied as a potential target to enhance radiosensitivity GBM. Methods Materials: Mouse GL-261 Human U87MG cells were used cell models. Clonogenic survival assays transwell invasion performed...
The aggressive nature and inherent therapeutic resistance of glioblastoma multiforme (GBM) has rendered the median survival afflicted patients to 14 months. Therefore, it is imperative understand molecular biology GBM provide new treatment options overcome this disease. It been demonstrated that protein kinase R-like endoplasmic reticulum (PERK) pathway an important regulator (ER) stress response. PERK signaling observed in other model systems after radiation; however, less known context...
Subependymal giant cell astrocytoma (SEGA) is a rare tumor occurring almost exclusively in patients with tuberous sclerosis complex. Although open resection remains the standard therapy, complication rates remain high. To minimize morbidity, less invasive approaches, such as endoscope-assisted resection, radiosurgery, and chemotherapy mTOR pathway inhibitors, are also used to treat these lesions. Laser interstitial thermal therapy (LITT) relatively new modality that increasingly variety of...
OBJECTIVE The precision of laser probe insertion for interstitial thermal therapy deep-seated lesions is limited by the method stereotactic guidance. objective this study was to evaluate feasibility customized STarFix 3D-printed platforms guide into mesiotemporal and posterior fossa targets. METHODS authors conducted a retrospective review 5 patients (12–55 years age) treated with (LITT) in which were used insertion. Bone fiducials implanted each patient's skull, subsequent CT scans design...
Cancer-specific targeting sparing normal tissues would significantly enhance cancer therapy outcomes and reduce cancer-related mortality. One approach is to target receptors or molecules that are specifically expressed on cells. Peptides as cancer-specific agents offer advantages such ease of synthesis, low antigenicity, enhanced diffusion into tissues. Glucose-regulated protein 78 (GRP78) an endoplasmic reticulum stress chaperone regulates the unfolded response overexpressed in various...
Non-small-cell lung cancer (NSCLC) and glioblastoma (GB) have poor prognoses. Discovery of new molecular targets is needed to improve therapy. Tax interacting protein 1 (TIP1), which plays a role in progression, overexpressed radiation-inducible NSCLC GB. We evaluated the effect an anti-TIP1 antibody alone combination with ionizing radiation (XRT) on GB vitro vivo. cells were treated antibodies for proliferation, colony formation, endocytosis, cell death. The efficacy XRT tumor growth was...
Journal Article High-throughput identification of putative receptors for cancer-binding peptides using biopanning and microarray analysis Get access Daniel J. Ferraro, Ferraro Department Radiation Oncology, Mallinckrodt Institute Radiology, Siteman Cancer Center, Washington University School Medicine, 4511 Forest Park, Saint Louis, MO 63110, USA Search other works by this author on: Oxford Academic Google Scholar Sandeep R. Bhave, Bhave Rama P. Kotipatruni, Kotipatruni Jeremy C. Hunn, Hunn...
Cluster headache (CH) is among the most common and debilitating autonomic cephalalgias. We characterize clinical outcomes of deep brain stimulation (DBS) to posterior hypothalamic region through a novel analysis electrophysiological topography tractography-based structural connectivity. The left hypothalamus was targeted ipsilateral refractory CH symptoms. Intraoperatively, field potentials were captured in 1mm depth increments. Whole-brain probabilistic tractography conducted assess...
Abstract Traditional radiation therapy is often associated with significant toxicity to normal cells that could limit the success of cancer therapy. The greater understanding in molecular differences between and has lead targeted therapies treatment. We have discovered novel radiation-inducible antigens using phage-display peptide libraries. One such inducible-antigen Glucose regulated protein 78kDa (GRP78) was shown bind hexapeptide GIRLRG. GRP78 known regulate cellular stresses, including...
Abstract Glioblastoma is the most common primary malignant brain tumor in adults United States. While standard of care for afflicted patients has evolved to include surgery, ionizing radiation (IR) and temozolomide chemotherapy, prognoses these remain dismal. With median survival glioblastoma remaining range 12-15 months, there an unmet need approaches that can overcome inherent therapeutic resistance tumors. In recent years, several studies have demonstrated IR activate pro-survival...
OBJECTIVE Brain metastases (BMs) secondary to sarcoma are rare, and their incidence ranges from 1% 8% of all bone soft tissue sarcomas. Although stereotactic radiosurgery (SRS) is widely used for BMs, only a few papers have reported on SRS metastasizing the brain. The purpose this study was evaluate safety effectiveness BM. METHODS authors retrospectively reviewed clinical radiological outcomes patients with BM histopathologically confirmed treated SRS, either as primary treatment or...
Abstract Antigens that are over-expressed in cancer response to radiation being used as novel targets. We showed tax interacting protein 1 (TIP-1) be radiation-inducible translocated the surface of cell following irradiation. TIP-1, which consists a single PDZ domain plays an important role signaling, development, and progression. TIP-1’s involvement various survival pathways makes it attractive target for anticancer therapeutics. antibodies specific this determine its survival. monitored...
Abstract Despite advances in diagnostic and therapeutic modalities, lung cancer remains the leading cause of cancer-related mortality United States. In most cases, presents as an unresectable mass requiring a combination chemotherapy radiotherapy (CRT) for treatment. A barrier to efficacy this treatment is radioresistance. Radiation induced activation pro-survival pathways are currently thought play important role The enzyme cytosolic phospholipase A2 (cPLA2) has been identified key...
Abstract Traditional radiation therapy is often associated with significant toxicity to normal cells that could limit the success of cancer therapy. The greater understanding in molecular differences between and has lead targeted therapies treatment. We have discovered novel radiation-inducible antigens using phage-display peptide libraries. One such inducible-antigen Glucose regulated protein 78kDa (GRP78) was shown bind hexapeptide GIRLRG. GRP78 known regulate cellular stresses, including...
Material and Methods: Chemically synthesized, RGD-/PEGfunctionalized gold nanoparticles (RGD:AuNP; ≈2 -3 nm) were characterized using STEM, TEM, LIBS imaging.Following clonogenic assay, radiation damage was induced in Panc1 xenografts with 10 Gy 220 kVp (Xtrahl, Inc).γ-H2AX, 3D-(confocal) vessel imaging IHC performed.Results: Tumor vessel-targeted subjected to conformal image-guided irradiation Panc-1 tumor xenograft induce vascular disruption.By specifically targeting the early angiogenic...
Abstract Therapies for poor prognosis cancers, such as lung cancer and glioblastoma, are limited due to radio-resistance tumor recurrence. Development of molecular targeted therapy can serve a potential method improve the efficacy radiation in both glioblastoma cancer. Ionizing (IR) activate series pro-survival pathways which contributes pathogenesis cells. Among these pathways, cytosolic phospholipase A2 (cPLA2) is an integral component activated by IR. Following activation, cPLA2 cleaves...