A5024897326- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Neurological disorders and treatments
- Neurological diseases and metabolism
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Cholinesterase and Neurodegenerative Diseases
- Ear Surgery and Otitis Media
- Signaling Pathways in Disease
- Genetic Neurodegenerative Diseases
- Parkinson's Disease and Spinal Disorders
- Biotin and Related Studies
- Frailty in Older Adults
- Apelin-related biomedical research
- Neurological Disorders and Treatments
- Phosphodiesterase function and regulation
- Botulinum Toxin and Related Neurological Disorders
- Ginkgo biloba and Cashew Applications
- Ubiquitin and proteasome pathways
- Biochemical effects in animals
- Hearing Loss and Rehabilitation
- Hearing, Cochlea, Tinnitus, Genetics
- Prion Diseases and Protein Misfolding
- Dementia and Cognitive Impairment Research
Mansoura University
2019-2025
Aarhus University
2019-2024
Abstract Here we describe the use of an organotypic hippocampal slice model for studying α-synuclein aggregation and inter-neuronal spreading initiated by microinjection pre-formed fibrils (PFFs). PFF injection at dentate gyrus (DG) templates formation endogenous aggregates in axons cell bodies this region that spread to CA3 CA1 regions. Aggregates are insoluble phosphorylated serine-129, recapitulating Lewy pathology features found Parkinson’s disease other synucleinopathies. The was favor...
Accumulation of aggregated alpha-synuclein (α-syn) is believed to play a pivotal role in the pathophysiology Parkinson's disease (PD) and other synucleinopathies. As key constituent Lewy pathology, more than 90% α-syn bodies phosphorylated at serine-129 (pS129) hence, it used extensively as marker for pathology. However, exact pS129 remains controversial kinase(s) responsible phosphorylation have yet be determined. In this study, we investigated effect Polo-like kinase 2 (PLK2) inhibition on...
Mice transgenic for human P301S tau protein exhibit many characteristics of the tauopathies, including formation abundant hyperphoshorylated filaments, associated neuroinflammation and disease phenotype. However, exact underpinning mechanisms are still not fully addressed that hinder our understanding tauopathy diseases development possible therapeutic targets.Methods: In current study, hippocampus from three time points (2, 4 6 months) mice were further characterized in comparison to age...
The impact of LRRK2 variants on the risk Parkinson's disease in Egyptians remains unknown. We examined 1210 (611 PD patients and 599 controls) from 16 governorates across Egypt for 12 pathogenic variants. p.Gly2019Ser was only variant detected, with a prevalence 4.1% sporadic cases, 6.5% familial 0.68% controls. Among carriers, all were heterozygous bar one homozygous patient, shared common haplotype 1. Demographics UPDRS scores did not differ between carriers non-carriers, most being males...
α-Synuclein proximity ligation assay (PLA) has proved a sensitive technique for detection of non-Lewy body α-synuclein aggregate pathology. Here, we describe the MJF-14 PLA, new PLA towards aggregated with unprecedented specificity, using aggregate-selective antibody MJFR-14-6-4-2 (hereafter MJF-14). Signal in correlates aggregation cell culture and human neurons, induced by overexpression or pre-formed fibrils. Co-labelling pS129-α-synuclein immunofluorescence post-mortem cases dementia...
Abstract Here we describe the use of an organotypic hippocampal slice model for studying α-synuclein aggregation and inter-neuronal spreading initiated by injection preformed filaments (PFFs). PFF at dentate gyrus templates endogenous to form aggregates in axons cell bodies that spread CA3 CA1 regions. Aggregates were insoluble phosphorylated serine 129, recapitulating Lewy pathology features found Parkinson’s disease other synucleinopathies. The favoring anterograde direction model. allowed...
Abstract Background Spinal cord injury (SCI) is widely considered the most disastrous medical condition. With no available treatment to date, SCI continues cause disabilities patients and affect their own caregivers' quality of life. Cerebrolysin (CBL) a neuropeptide preparation derived from purified brain proteins with suggested neuroprotective neurotrophic properties. CBL showed earlier ability target multiple pathways that helped in improvement recovery following different groups...
Breast cancer is the most common malignancy globally, and considered a major cause of cancer-related death. Tremendous effort exerted to identify an optimal anticancer drug with limited side effects. The quinoline derivative RIMHS-Qi-23 had wide-spectrum antiproliferative activity against various types cells.In current study, effect was tested on MCF-7 breast cell line evaluate its efficacy in comparison reference compound doxorubicin.Our data suggest anti-proliferative superior potency...
Abstract Accumulation of aggregated alpha-synuclein (α-syn) is believed to play a pivotal role in the pathophysiology Parkinson’s disease (PD) and other synucleinopathies. α-Syn key constituent protein Lewy pathology, α-syn phosphorylated at serine-129 (pS129) constitutes more than 90% bodies hence, it used extensively as pathological marker for form α-syn. However, exact pS129 remains controversial well kinase(s) responsible phosphorylation. In this study, we investigated effect Polo-like...
Parkinson's disease (PD) is the second most common neurodegenerative after Alzheimer's disease. PD has been traditionally considered a motoric disorder characterized by tremor, rigidity and bradykinesia, however it now settled that also comprises range of non-motor symptoms like hyposmia, sleep disturbances cognitive impairments (Tysnes Storstein, 2017). The pathological hallmark presence neuronal cytoplasmic inclusions called Lewy bodies (LB) or neurites, which consist abundant presynaptic...
<title>Abstract</title> The impact of <italic>LRRK2</italic> variants on the risk Parkinson's disease (PD) in Egyptians remains unknown. We examined 1,210 (611 PD patients and 599 controls) for 12 mutations. p.Gly2019Ser was only variant detected across Egypt, with a prevalence 4.1% sporadic cases, 6.5% familial 0.68% controls. Among carriers, all were heterozygous bar one homozygous patient, shared common haplotype 1. Demographics UPDRS scores did not differ between carriers non-carriers,...
Abstract Lewy pathology, consisting of bodies and neurites, is the pathological hallmark synucle-inopathies such as Parkinson’s disease dementia with bodies, but it generally thought to represent late-stage changes. In contrast, α-synuclein oligomers are regarded early-stage likely involved in progression cellular toxicity. Oligomers, however, not de-tected by standard immunohistochemistry require specific detection techniques proxim-ity ligation assay (PLA). Here, we describe MJF-14 PLA, a...
Abstract Background Rheumatoid arthritis (RA) is an inflammatory autoimmune disorder that has cardinal articular and peri-articular symptoms. Extra-articular manifestations (EAMs) are also reported among RA patients. In the current study, we assessed hearing function in 50 An extensive audiological assessment including pure tone audiometry (PTA), extended high-frequency (EHFA), tympanometry, acoustic reflex addition to oto-acoustic emission (OAEs) were done. Results Our data demonstrates...