A5062249568- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Nanopore and Nanochannel Transport Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Protein purification and stability
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Analytical chemistry methods development
- Electrowetting and Microfluidic Technologies
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Graphene and Nanomaterials Applications
- Chromatography in Natural Products
- Glycosylation and Glycoproteins Research
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Mercury impact and mitigation studies
- DNA and Nucleic Acid Chemistry
- Melamine detection and toxicity
- Identification and Quantification in Food
- Electrostatics and Colloid Interactions
- Advanced Chemical Sensor Technologies
- Supramolecular Self-Assembly in Materials
University of Oklahoma
2011-2019
Lanzhou University
2009-2010
This paper describes an approach to determine melamine (MEL) in liquid milk, yogurt, whole milk powder, fish feed, and at residue levels using capillary zone electrophoresis with diode array detection (CZE-DAD) for the first time. Suspicious samples were extracted 1% trichloroacetic acid while 1 mL of chloroform was used precipitate fat real samples. After centrifuging filtering, extract analyzed by CZE-DAD directly. By investigating variables extraction, separation, detail, entire...
The pressure output of a pump cannot be increased simply by connecting several them in series. This barrier is eliminated with the micropump developed this work. actually an assembly number fundamental units connected maximum directly proportional to serially units. Theoretically, one can always enhance adding more assembly, but reality upper constrained microtees or microunions joining components. With commercially available and microunions, pressures than 1200 bar have been achieved. We...
Pressure-induced transport of double-stranded DNA (dsDNA) from 10 base pairs (bp) to 1.9 mega (Mbp) confined in a 750-nm-radius capillary was studied using hydrodynamic chromatographic technique and four distinct length regions (rod-like, free-coiled, constant mobility, transition regions) were observed. The behavior varied closely with region changes. rod-like consisted shorter than the persistence (∼150 bp) dsDNA, these molecules behaved like polymer rods. Free-coiled ∼150 bp ∼2 kilo...
Sodium dodecyl sulfate (SDS)−polyacrylamide gel electrophoresis (PAGE) is a fundamental analytical technique for proteomic research, and SDS−capillary (CGE) its miniaturized version. Compared to conventional slab-gel electrophoresis, SDS−CGE has many advantages such as increased separation efficiency, reduced time, automated operation. not widely accepted in research primarily due the difficulties identifying well-resolved proteins. MALDI-TOF-MS an outstanding platform protein...
In this work, we overcame the deficiencies of large volume sample stacking (LVSS) in separating low-mobility and neutral analytes through combining LVSS with sweeping CE, employed new approach to enrich separate anionic simultaneously. This technique was carried out pressure injection large-volume followed by EOF as a pump pushing bulk low-conductivity matrix outlet capillary while were swept micelles separated via MEKC without electrode polarity switching. Careful optimization enrichment...
Turn the volume down: Coupling a high-pressure electroosmotic pump (EOP) and microfabricated chip-injector with bare narrow capillary–hydrodynamic chromatographic system (BaNC-HDC), enables samples to be injected at low-picoliter volumes, analytes eluted picoliters per minute, wide size range of DNA fragments resolved (see picture) rapidly in free solution single-molecule level. As service our authors readers, this journal provides supporting information supplied by authors. Such materials...
Multiplex polymerase chain reaction (PCR) has been widely utilized for high-throughput pathogen identification. Often, a dye is used to intercalate the amplified DNA fragments, and identifications of pathogens are carried out by melting curve analysis or gel electrophoresis. Integrating amplification identification logic path toward maximizing benefit multiplex PCR. Although PCR electrophoresis have integrated, replenishing gels after each run tedious time-consuming. In this technical note,...
We report a novel approach for effectively separating DNA molecules in free solution. The method uses bare narrow open capillary without any sieving matrices to resolve wide size-range of fragments at efficiencies more than million plates per meter routinely.
Abstract Determining the sizes and measuring quantities of DNA molecules are fundamental tasks in molecular biology. usually evaluated by gel electrophoresis, but this method cannot simultaneously size quantitate a at low zeptomole (zmol) levels concentration. We have recently developed new technique, called bare‐narrow‐capillary/hydrodynamic‐chromatography or BaNC‐HDC, for resolving based on their without using any sieving matrices. In report, we utilize BaNC‐HDC fragments zmol to...
In this paper, we develop a simple electrokinetic means for fractionating protein samples according to their pI values without using ampholytes. The method uses inexpensive equipment, and its consumables are primarily ammonium acetate buffers. A key component of apparatus is dialysis membrane interface that eliminates electrolysis-caused oxidation/reduction constrains proteins in the desired places. We demonstrate feasibility standard real-world samples. With elimination ampholytes, can...
Kleinigkeit! Durch Koppeln einer elektroosmotischen Hochdruckpumpe (EOP) und eines Mikrochipinjektors mit freiliegenden engen Kapillaren einem hydrodynamischen Chromatographiesystem (BaNC-HDC) können Pikoliter-Proben injiziert, Analyte in Pikolitervolumina pro Minute eluiert DNA-Fragmente weiten Größenspektrum schnell Lösung auf der Einzelmolekülebene getrennt werden (siehe Bild). As a service to our authors and readers, this journal provides supporting information supplied by the authors....