A5075078183- Antimicrobial Resistance in Staphylococcus
- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
- Immune Response and Inflammation
- Sphingolipid Metabolism and Signaling
- Bacterial biofilms and quorum sensing
- NF-κB Signaling Pathways
- Restless Legs Syndrome Research
- Bacterial Identification and Susceptibility Testing
- Antifungal resistance and susceptibility
- Endoplasmic Reticulum Stress and Disease
- Microbial infections and disease research
- Parkinson's Disease Mechanisms and Treatments
- Blood disorders and treatments
- Lysosomal Storage Disorders Research
- Antimicrobial Peptides and Activities
- Allergic Rhinitis and Sensitization
- Phagocytosis and Immune Regulation
- Pharmacological Effects of Natural Compounds
- Infections and bacterial resistance
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
University of Cologne
2009-2021
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2019-2021
University Hospital Cologne
2015-2021
ABSTRACT Staphylococcus aureus is an important human pathogen with increasing clinical impact due to the extensive spread of antibiotic-resistant strains. Therefore, development a protective polyvalent vaccine great interest. We employed intravenous immunoglobulin (IVIG) preparation as source antibodies directed against anchorless S. surface proteins for identification novel candidates. In order identify such proteins, subtractive proteome analysis (SUPRA) cell wall was performed. Proteins...
Abstract Although numerous pathogenic changes within the mitochondrial respiratory chain (RC) have been associated with an elevated occurrence of apoptosis affected tissues, mechanistic insight into how dysfunction initiates apoptotic cell death is still unknown. In this study, we show that specific alteration cytochrome c oxidase (COX), representing a common defect found in diseases, facilitates response to oxidative stress. Our data identified increased ceramide synthase 6 (CerS6) activity...
Abstract Loss of skeletal muscle mass is one the most widespread and deleterious processes in aging humans. However, mechanistic metabolic principles remain poorly understood. In framework a multi‐organ investigation age‐associated changes ceramide species, unique distinctive change pattern C 16:0 18:0 species was detected aged muscle. Consistently, expression CerS1 CerS5 mRNA, encoding synthases (CerS) with substrate preference for acyl chains, respectively, down‐regulated mice. Similarly,...
Abstract Staphylococcus aureus represents a serious infectious threat to global public health and vaccine against S. an unmet medical need. We here characterise two candidates, coproporphyrinogen III oxidase (CgoX) triose phosphate isomerase (TPI), which fulfil essential housekeeping functions in heme synthesis glycolysis, respectively. Immunisation with rCgoX rTPI elicited protective immunity bacteremia. Two monoclonal antibodies (mAb), CgoX-D3 TPI-H8, raised CgoX TPI, efficiently provided...
The enormous capacity of Staphylococcus aureus to acquire antibiotic resistance makes it a permanent task search for and develop new anti-infectives. One the possible approaches is early active immunization risk patients animal stocks prevent S. infections. Based on proteome screen with aureus-specific human antiserum, we have previously identified several anchorless cell wall proteins be used as novel vaccine candidates. To an efficient anti-S. vaccine, supplemented adjuvants Montanide(™)...
Although the crucial role of professional phagocytes for clearance S. aureus infections is well-established, several studies indicate an adverse leukocytes in dissemination during infection. Since only little known about macrophages this context, we analyzed macrophages, and particular reactive oxygen species deficiency, seeding metastases. Infection bone marrow-derived (BMDM) with revealed that NADPH oxidase 2 (NOX2-) deficient, but not NOX1- or NOX4-deficient, BMDM failed to clear...