A5036240208- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Vibrio bacteria research studies
- Silk-based biomaterials and applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Redox biology and oxidative stress
- Retinal Diseases and Treatments
- Biofuel production and bioconversion
- Bone Tissue Engineering Materials
- Renal and related cancers
- RNA Interference and Gene Delivery
- Advanced Glycation End Products research
- Immune cells in cancer
- Virus-based gene therapy research
- Glaucoma and retinal disorders
- Immune Response and Inflammation
- Adipokines, Inflammation, and Metabolic Diseases
- Cannabis and Cannabinoid Research
- interferon and immune responses
- Bioactive Compounds and Antitumor Agents
- Nitric Oxide and Endothelin Effects
- Retinal Imaging and Analysis
- Extracellular vesicles in disease
- Moringa oleifera research and applications
University of Cologne
2013-2025
University Hospital Cologne
2019-2025
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2019-2024
German Center for Infection Research
2024
Institute of Medical Microbiology and Hygiene
2019-2021
A major function of macrophages during infection is initiation the proinflammatory response, leading to secretion cytokines that help orchestrate immune response. Here, we identify reactive oxygen species (ROS) as crucial mediators signaling cytokine in Listeria monocytogenes-infected macrophages. ROS produced by NADPH oxidases (Noxes), such Nox2, are key components macrophage response invading pathogens; however, our data show mediated were mitochondria (mtROS). We identified inhibitor κB...
Reactive oxygen species (ROS), such as the superoxide anion or hydrogen peroxide, have been established over decades of research as, on one hand, important and versatile molecules involved in a plethora homeostatic processes and, other inducers damage, pathologies diseases. Which effects ROS induce, strongly depends cell type source, amount, duration location production. Similar to cellular pH calcium levels, which are both strictly regulated only altered by when necessary, redox balance is...
Abstract Aberrant immune responses including reactive phagocytes are implicated in the etiology of age-related macular degeneration (AMD), a major cause blindness elderly. The translocator protein (18 kDa) (TSPO) is described as biomarker for gliosis, but its biological functions retinal diseases remain elusive. Here, we report that tamoxifen-induced conditional deletion TSPO resident microglia using Cx3cr1 CreERT2 :TSPO fl/fl mice or targeting with synthetic ligand XBD173 prevents...
In this study, the novel biomimetic aerogel-based composite scaffolds through a synergistic combination of wet chemical synthesis and advanced engineering approaches have successfully designed. To aim, initially photo-crosslinkable methacrylated silk fibroin (SF-MA) biopolymer hollow mesoporous silica microcapsules (HMSC-MA) as main constituents aerogels were synthesized. Afterward, by incorporation drug-loaded HMSC-MA into self-assembled SF-MA, printable gel-based inks are developed. By...
Biomaterial-mediated bone tissue engineering (BTE) offers an alternative, interesting approach for the restoration of damaged tissues in postsurgery osteosarcoma treatment. This study focused on synthesizing innovative composite inks, integrating self-assembled silk fibroin (SF), tannic acids (TA), and electrospun bioactive glass nanofibers 70SiO2-25CaO-5P2O5 (BGNF). By synergistically combining unique characteristics these three components through self-assembly microextrusion-based...
In this study we develop novel type of antibacterial chitosan-propolis NPs to improve theantimicrobial activity against various pathogens. To aim, primarily extracted propolis with methylal and ethanol as green solvents its encapsulation chitosan NPs. The developed loaded indicated antimicrobial anti-biofilm properties gram positive negative. FTIR revealed the successful extract Ethanol (PE) Methylal (PM) into nano career matrix. HPLC GC-MASS also confirmed presence flavonoids phenols...
Riboflavin, also known as vitamin B2 , is converted by riboflavin kinase (RFK) into flavin mononucleotide (FMN) and adenine dinucleotide (FAD), which are essential cofactors of dehydrogenases, reductases, oxidases including the phagocytic NADPH oxidase 2 (Nox2). Riboflavin deficiency common in young adults elderly individuals, who at coincidental risk for listeriosis. To address impact acute on host defense against Listeria monocytogenes (L.m.), we generated conditional RFK knockout (KO)...
Abstract Corticosteroids are host-directed drugs with proven beneficial effect on survival of tuberculosis (TB) patients, but their precise mechanisms action in this disease remain largely unknown. Here we show that corticosteroids such as dexamethasone inhibit necrotic cell death cells infected Mycobacterium (Mtb) by facilitating mitogen-activated protein kinase phosphatase 1 (MKP-1)-dependent dephosphorylation p38 MAPK. Characterization mixed lineage domain-like (MLKL) and tumor necrosis...
Age-related macular degeneration (AMD) is a leading cause of visual impairment in the elderly. The neovascular (wet) form AMD can be treated with intravitreal injections different anti-vascular endothelial growth factor (VEGF) agents. Placental (PGF) another member VEGF family cytokines pro-angiogenic and pro-inflammatory effects. Here, we aimed to compare single combined inhibition VEGF-A PGF laser-induced mouse model choroidal neovascularization (CNV) focus on effects retinal mononuclear...
Non-canonical autophagy pathways decorate single-membrane vesicles with Atg8-family proteins such as MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3). Phagosomes containing the bacterial pathogen Listeria monocytogenes (L.m.) can be targeted by a non-canonical pathway called LC3-associated phagocytosis (LAP), which substantially contributes to anti-listerial activity of macrophages and immunity. We here characterized second targeting L.m.-containing phagosomes, is induced damage...
Abstract Background NADPH oxidase (NOX), a primary source of endothelial reactive oxygen species (ROS), is considered key event in disrupting the integrity blood-retinal barrier. Abnormalities neurovascular-coupled immune signaling herald loss ganglion cells glaucoma. Persistent microglia-driven inflammation and cellular innate system dysregulation often lead to deteriorating retinal degeneration. However, crosstalk between NOX environment remains unresolved. Here, we investigate interaction...
Macrophages are phagocytic cells specialized in detecting molecules of non-self origin. To this end, they equipped with a large array pattern recognition receptors (PRRs). Unfortunately, also makes macrophages particularly challenging to transfect as the transfection reagent and transfected nucleic acids often recognized by PRRs non-self. Therefore, results macrophage activation degradation or even suicide macrophages. Here, we describe protocol that allows highly efficient murine primary...
Macrophages remove bacteria from the extracellular milieu via phagocytosis. While most of engulfed are degraded in antimicrobial environment phagolysosome, several bacterial pathogens have evolved virulence factors, which evade degradation or allow escape into cytosol. To counter this situation, macrophages activate LC3-associated phagocytosis (LAP), a highly bactericidal non-canonical autophagy pathway, destroys so called LAPosomes. Moreover, can also target intracellular by pore-forming...
Macrophages are phagocytic cells specialized in detecting molecules of non-self origin. To this end, they equipped with a large array pattern recognition receptors (PRRs). Unfortunately, also makes macrophages particularly challenging to transfect as the transfection reagent and transfected nucleic acids often recognized by PRRs non-self. Therefore, results macrophage activation degradation or even suicide macrophages. Here, we describe protocol that allows highly efficient murine primary...
Although the crucial role of professional phagocytes for clearance S. aureus infections is well-established, several studies indicate an adverse leukocytes in dissemination during infection. Since only little known about macrophages this context, we analyzed macrophages, and particular reactive oxygen species deficiency, seeding metastases. Infection bone marrow-derived (BMDM) with revealed that NADPH oxidase 2 (NOX2-) deficient, but not NOX1- or NOX4-deficient, BMDM failed to clear...
The aim of this study is to design a therapeutic enhanced three-dimensional (3D) silk fibroin (SF)-based scaffold containing propolis (Ps)-loaded chitosan (CH) nanocarriers. To aim, we initially synthesized hybrid gel-based ink by synergistic sol-gel and self-assembly approach then processed the resulting gels microextrusion-based 3D printing followed supercritical drying obtain aerogel scaffolds. Ps was utilized enhance final scaffold's bactericidal efficacy cell responsiveness. For...