A5022817205- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mechanical and Optical Resonators
- Mass Spectrometry Techniques and Applications
- Immune Response and Inflammation
- Autophagy in Disease and Therapy
- Analytical Chemistry and Sensors
- Advanced MEMS and NEMS Technologies
- Atomic and Molecular Physics
- Biofuel production and bioconversion
- Neuropeptides and Animal Physiology
- Ion-surface interactions and analysis
- Sphingolipid Metabolism and Signaling
- Virus-based gene therapy research
- Lipid Membrane Structure and Behavior
- Vibrio bacteria research studies
- Advancements in Semiconductor Devices and Circuit Design
- Inflammasome and immune disorders
- Phagocytosis and Immune Regulation
- Advanced Chemical Physics Studies
- Redox biology and oxidative stress
- Physics and Engineering Research Articles
- RNA Interference and Gene Delivery
- Renal and related cancers
- Pain Mechanisms and Treatments
- Semiconductor materials and devices
University of Cologne
1975-2021
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2019-2021
University Hospital Cologne
2019-2021
Institute of Medical Microbiology and Hygiene
2009-2021
Chemnitz University of Technology
2010-2015
Karolinska Institutet
1994-1997
A major function of macrophages during infection is initiation the proinflammatory response, leading to secretion cytokines that help orchestrate immune response. Here, we identify reactive oxygen species (ROS) as crucial mediators signaling cytokine in Listeria monocytogenes-infected macrophages. ROS produced by NADPH oxidases (Noxes), such Nox2, are key components macrophage response invading pathogens; however, our data show mediated were mitochondria (mtROS). We identified inhibitor κB...
Reactive oxygen species (ROS), such as the superoxide anion or hydrogen peroxide, have been established over decades of research as, on one hand, important and versatile molecules involved in a plethora homeostatic processes and, other inducers damage, pathologies diseases. Which effects ROS induce, strongly depends cell type source, amount, duration location production. Similar to cellular pH calcium levels, which are both strictly regulated only altered by when necessary, redox balance is...
Abstract Aberrant immune responses including reactive phagocytes are implicated in the etiology of age-related macular degeneration (AMD), a major cause blindness elderly. The translocator protein (18 kDa) (TSPO) is described as biomarker for gliosis, but its biological functions retinal diseases remain elusive. Here, we report that tamoxifen-induced conditional deletion TSPO resident microglia using Cx3cr1 CreERT2 :TSPO fl/fl mice or targeting with synthetic ligand XBD173 prevents...
Scaffold-mediated tissue engineering has become a golden solution for the regeneration of damaged bone tissues that lack self-regeneration capability. A successful scaffold in comprises multitude suitable biological, microarchitectural, and mechanical properties acting as different signaling cues cells to mediate new formation. Therefore, careful design bioactive macro- microstructures multiple length scales biophysical fulfilling repair demands are necessary yet challenging achieve. Herein,...
Riboflavin, also known as vitamin B2 , is converted by riboflavin kinase (RFK) into flavin mononucleotide (FMN) and adenine dinucleotide (FAD), which are essential cofactors of dehydrogenases, reductases, oxidases including the phagocytic NADPH oxidase 2 (Nox2). Riboflavin deficiency common in young adults elderly individuals, who at coincidental risk for listeriosis. To address impact acute on host defense against Listeria monocytogenes (L.m.), we generated conditional RFK knockout (KO)...
Abstract Corticosteroids are host-directed drugs with proven beneficial effect on survival of tuberculosis (TB) patients, but their precise mechanisms action in this disease remain largely unknown. Here we show that corticosteroids such as dexamethasone inhibit necrotic cell death cells infected Mycobacterium (Mtb) by facilitating mitogen-activated protein kinase phosphatase 1 (MKP-1)-dependent dephosphorylation p38 MAPK. Characterization mixed lineage domain-like (MLKL) and tumor necrosis...
Non-canonical autophagy pathways decorate single-membrane vesicles with Atg8-family proteins such as MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3). Phagosomes containing the bacterial pathogen Listeria monocytogenes (L.m.) can be targeted by a non-canonical pathway called LC3-associated phagocytosis (LAP), which substantially contributes to anti-listerial activity of macrophages and immunity. We here characterized second targeting L.m.-containing phagosomes, is induced damage...
The acid sphingomyelinase (ASMase) localizes to the lumen of endosomes, phagosomes and lysosomes as well outer leaflet plasma membrane hydrolyses sphingomyelin ceramide phosphorylcholine. Using facultative intracellular bacterium Listeria monocytogenes, we show that maturation into phagolysosomes is severely impaired in macrophages genetically deficient for ASMase. Unlike wild-type macrophages, containing L. monocytogenes ASMase(-/-) remained positive late phagosomal markers...
The influence of mechanical stress on field effect transistors is investigated using a pressure‐deflected membrane for generation various stresses. It consists silicon and transistors, which are designed manufactured 1.0 μm‐XC10 technology from X‐Fab. transducers sensing placed the edges with maximum stress. Furthermore, position optimized by FEM simulations (Ansys). Different variances impact their electrical properties investigated. Transistors different parameters such as channel lengths,...
Macrophages are phagocytic cells specialized in detecting molecules of non-self origin. To this end, they equipped with a large array pattern recognition receptors (PRRs). Unfortunately, also makes macrophages particularly challenging to transfect as the transfection reagent and transfected nucleic acids often recognized by PRRs non-self. Therefore, results macrophage activation degradation or even suicide macrophages. Here, we describe protocol that allows highly efficient murine primary...
Deep trenches for device insulation in a high-voltage process thick SOI were fabricated using different manufacturing technologies. The have been investigated by current-voltage-characteristics. In comparison to the conventional produced alternatively samples reach remarkable increase of breakdown voltages accompanied decline leakage current order several magnitudes. Respecting other parameters trench fabrication method has selected which enables reliable single suitable operating up 650 V....
Macrophages are phagocytic cells specialized in detecting molecules of non-self origin. To this end, they equipped with a large array pattern recognition receptors (PRRs). Unfortunately, also makes macrophages particularly challenging to transfect as the transfection reagent and transfected nucleic acids often recognized by PRRs non-self. Therefore, results macrophage activation degradation or even suicide macrophages. Here, we describe protocol that allows highly efficient murine primary...
Although the crucial role of professional phagocytes for clearance S. aureus infections is well-established, several studies indicate an adverse leukocytes in dissemination during infection. Since only little known about macrophages this context, we analyzed macrophages, and particular reactive oxygen species deficiency, seeding metastases. Infection bone marrow-derived (BMDM) with revealed that NADPH oxidase 2 (NOX2-) deficient, but not NOX1- or NOX4-deficient, BMDM failed to clear...
<div class="section abstract"><div class="htmlview paragraph">Electrified vehicles represent mobility’s future, but they impose challenging and diverse requirements like range performance. To meet these requirements, various components, such as battery cells, electric drives, fuel hydrogen vessels need to be integrated into a drive storage system that optimizes the key performance indicators (KPI). However, finding best combination of components is multifaceted problem in early...