A5062582914- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Lysosomal Storage Disorders Research
- Pancreatic function and diabetes
- Neonatal Respiratory Health Research
- Genetics and Neurodevelopmental Disorders
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Microbial Metabolites in Food Biotechnology
- Congenital Diaphragmatic Hernia Studies
- Ubiquitin and proteasome pathways
- Infant Development and Preterm Care
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Pharmacogenetics and Drug Metabolism
- Urological Disorders and Treatments
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Birth, Development, and Health
- Studies on Chitinases and Chitosanases
- Urinary and Genital Oncology Studies
- Ureteral procedures and complications
- Analytical Chemistry and Chromatography
- Lipid Membrane Structure and Behavior
- DNA and Nucleic Acid Chemistry
University of Canterbury
2020-2024
Gunma University
2015-2024
Kiryu University
2015-2024
Matsuyama University
2017
Tokyo Institute of Technology
2016
Children's Medical Center
2012
Social Welfare Organization Saiseikai Imperial Gift Foundation
2007
Tokyo Medical University
1967-2001
Tokyo Metropolitan Komagome Hospital
1994-1995
Tokyo Metropolitan Institute of Medical Science
1994-1995
Abstract We developed a fluorescence‐quenching‐based assay system to determine the hydrolysis activity of endo ‐β‐ N ‐acetylglucosaminidases (ENGases). The pentasaccharide derivative 1 was labeled with an ‐methylanthraniloyl group as reporter dye at non‐reducing end and 2,4‐dinitrophenyl quencher molecule reducing end. This is hydrolyzed by ENGase, resulting in increase fluorescence intensity. Thus, signal directly proportional amount tetrasaccharide derivative, hence allowing ENGase be...
Glycans from microbial pathogens are well known pathogen-associated molecular patterns that recognized by the host immunity; however, little is about whether and how mammalian self-glycans activate immune response, especially in context of autoimmune disease. Using biochemical fractionation two-dimensional HPLC, we identify an abundant bioactive free glycan, Manβ1-4GlcNAc disaccharide TREX1-associated diseases. We report both monosaccharide residues β1-4 linkage critical for bioactivity this...
Morquio B disease was found in a 15‐year‐old Japanese boy who presented with progressive generalized skeletal dysplasia without neurological manifestations. Mild keratan sulfaturia found, and β‐galactosidase deficient fibroblasts. Gene analysis revealed two mutant alleles, 83 Tyr→ His (Y83H) 482 Arg→Cys (R482C). The former expressed low enzyme activity (2–5% of normal), the latter no detectable activity.
Abstract To demonstrate the structural specificity of glycosyl donor for transglycosylation reaction by using endo ‐β‐ N ‐acetylglucosaminidase from Mucor hiemalis ( ‐M), a series tetrasaccharide oxazoline derivatives was synthesized. These correspond to core structure an asparagine‐linked glycoprotein glycan with β‐mannose unit non‐natural‐type monosaccharide, including β‐glucose, β‐galactose, and β‐talose in place moiety. The activity wildtype (WT) ‐M two mutants, N175Q N175A, examined...
The glycosylation of unprotected carbohydrates has emerged as an area significant interest because it obviates the need for long reaction sequences involving protecting-group manipulations. Herein, we report one-pot synthesis anomeric glycosyl phosphates through condensation with phospholipid derivatives while retaining high stereo- and regioselective control. center was activated using 2-chloro-1,3-dimethylimidazolinium chloride to facilitate glycerol-3-phosphate in aqueous solution. A...
Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed triphosphate (UcmTP). UcmTP was incorporated correctly into long RNAs, its fidelity during using SuperScript III sufficient for RNA aptamer selection.
Abstract1. Hepatic drug metabolism was investigated in normal, adjuvant-induced arthritic (AA), indomethacin-treated AA and prednisolone-treated rats. The contents of P450 b5 the activities NADH-b5 reductase (fp2), NADPH-ferrihaemoprotein reductase, mixed function oxidase, FAD-monooxygenase several enzymes involved conjugation were remarkably lower than normal rats.2. Many decreased enzyme restored to levels by continuous administration (3 weeks) indomethacin or prednisolone, which improved...
Lubricants are essential additives in tablet formulations. Magnesium stearate (Mg-St) is the most commonly used lubricant tableting. Here, we sucrose fatty acid ester (SE) as an additive to manufacture tablets by direct compression. We evaluated effects of hydrophile–lipophile balance (HLB) and amount SE on flowability a pharmaceutical powder using angle repose practical internal friction measurements. In addition, investigated properties. When SEs with HLB ≥3 were added, was approximately...
Abstract Golgi endo ‐α‐mannosidase (G‐EM) catalyzes an alternative deglucosylation process for N‐glycans and plays important roles in the post‐endoplasmic reticulum (ER) quality control pathway. To understand post‐ER mechanism, we synthesized a tetrasaccharide probe detection of hydrolytic activity G‐EM based on fluorescence quenching assay. The was labeled with N ‐methylanthraniloyl group as reporter dye at non‐reducing end 2,4‐dinitrophenyl quencher reducing end. This is hydrolyzed to...
Abstract Lyso ‐phosphatidyl β‐D‐glucoside (lysoPtdGlc) is during embryonic development a neurite growth cone guidance cue associated with G‐protein coupled receptor 55, and has been found to be involved in variety of physiological pathological events, including inflammatory pain, cancer. Hence, the ability prepare large quantities lysoPtdGlc an important objective. Herein, we report convergent chemical synthesis its glyceryl epimer ( S ‐lysoPtdGlc). High stereocontrol β‐glycosyl H...
Recent studies demonstrated the occurrence of sialyl free N-glycans (FNGs) in sera from a variety animals. Unlike intracellular FNGs that mainly carry single N-acetylglucosamine at their reducing termini (Gn1-type), these extracellular have an N,N'-diacetylchitobiose (Gn2-type). The detailed mechanism for how they are formed, however, remains unclarified. In this study, we report on improved method isolating and found that, not only FNGs, but also neutral present animal sera. Most...
A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N'-diacetylchitobiose and Asn side-chain of N-intein peptides products react rapidly with C-intein by protein trans-splicing generate an active luciferase.
The cytosolic peptide:N-glycanase (PNGase) is involved in the quality control of N-glycoproteins via endoplasmic reticulum-associated degradation (ERAD) pathway. Mutations gene encoding PNGase (NGLY1 humans) cause NGLY1 deficiency. Recent findings indicate that F-box protein FBS2 SCF