Background: Oral cancer is one of the most prevalent cancers worldwide. Despite that oral cavity easily accessible for clinical examinations, are often not promptly diagnosed. Furthermore, to date no effective biomarkers available cancer. Therefore, there an urgent need identify novel able improve both diagnostic and prognostic strategies. In this context, development innovative high-throughput technologies molecular epigenetics analyses has generated a huge amount data may be used...

Uveal melanoma (UM) represents the most frequent primary tumor of eye. Despite development new drugs and screening programs, prognosis patients with UM remains poor no effective prognostic biomarkers are yet able to identify high‑risk patients. Therefore, in present study, microRNA (miRNA or miR) expression data, contained TCGA (UVM) database, were analyzed order a set miRNAs significance be used as clinical practice. Patients stratified into 2 groups, including stage (high‑grade vs....

Abstract Background Gut microbiota modulation has been demonstrated to be effective in protecting patients against detrimental effects of anti-cancer therapies, as well improve the efficacy certain treatments. Among most characterized probiotics, Lactobacillus rhamnosus GG (LGG) is currently utilized clinics alleviate diarrhea, mucositis or intestinal damage which might associated with several triggers, including Clostridium difficile infections, inflammatory gut diseases, antibiotic...

This study investigated the DNA damage and apoptosis in colon cancer cells HCT-116 Caco-2 induced by engineered titanium dioxide nanoparticles (TiO2-NPs) (60 nm) food additive E171. MTT assays showed that both chemical forms significantly reduced cell viability a dose-dependent manner. In particular E171 pronounced inhibitory effect on growth of lines (E171 IC50: 3.45 mg/L for HTC-116 1.88 Caco-2; TiO2-NPs 60 nm 41.1 14.3 Caco-2). A low level genotoxicity was observed cells, especially when...

The BRAFV600E mutation is associated with melanoma development and its detection in circulating-free DNA cannot be observed all patients harboring this tumor specimens. Beside the mutation, other markers of therapeutic response should identified. Matrix metalloproteinase-9 (MMP-9) could one them as role indicator invasiveness have been explored. In study, MMP-9 was evaluated cells after treatment dabrafenib. vitro data were validated 26 patients, which 14 treated BRAF inhibitor alone 12 both...

Tumor spreading is associated with the degradation of extracellular matrix proteins, mediated by overexpression metalloproteinase 9 (MMP-9). Although, such was linked to epigenetic promoter methylation, role intragenic methylation not clarified yet. Melanoma used as tumor model investigate relationship between DNA MMP9 gene and MMP-9 at transcriptional protein levels. Computational analysis revealed hypermethylation within CpG-2 region in melanoma samples high transcript In vitro validation...

Bladder cancer is one of the leading urinary tract. It often diagnosed at advanced stage disease. To date, no specific and effective early detection biomarkers are available. Cancer development progression associated with involvement both epithelial-mesenchymal transition (EMT) tumor microenvironment which NGAL/MMP-9 complex represents main player in bladder cancer. known that change microRNAs (miRNAs) expression may result gene modulation. Therefore, identification miRNAs EMT pathway be...

Pomegranate is an important source of several bioactive compounds with beneficial properties. As widely described in the literature, pomegranate can be considered as a functional food able to promote maintenance good health status. The present review article summarizes properties pomegranate, such antioxidant, anti‑inflammatory, anti‑aging, prebiotic and anticancer effects, well its protective role against metabolic disorders cardiovascular diseases. phytocompounds prevent development...

The targeting of the Mitogen-Activated Protein Kinase (MAPK) signalling pathway in melanoma improves prognosis patients harbouring V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) mutation. However, a fraction these may experience tumour progression due to resistance targeted therapy. Mutations affecting Phosphoinositol-3-Kinase (PI3K)-Akt favour onset drug resistance, suggesting existence crosstalk between MAPK and PI3K-Akt pathways. We hypothesized that inhibition both pathways be...

This study provides a first insight on the chemical characterization and quantification of silver nanoparticles (AgNPs) dissolved Ag in processed canned seafood products, where food-grade edible (E174) is not intentionally added nor nanoparticle contained food contact material. The aim was to evaluate bioaccumulation potential AgNPs contribute assessment ionic human dietary intake from seafood. It known how seafood, particular pelagic fish, precious nutritional source unsaturated fatty...

FBLN-3 has recently been proposed as a biomarker for malignant mesothelioma. A significantly increased standardized mortality rate from mesothelioma reported in Biancavilla, Italy. Its cause identified environmental exposure to fluoro-edenite. The aim of this study was seek correlation between plasma concentration and pleural plaques subjects exposed fluoro-edenite nonexposed control group. Pleural never detected the group, whereas it found 52% subjects. Median concentrations were 12.96 5.29...

Exposure to asbestos is associated with the development of mesothelioma. In addition asbestos, other fibers have been identified as risk factors for malignant and non-malignant diseases lungs. Among these, fluoro-edenite (FE) was found in patients from Biancavilla (Sicily, Italy) pleural lung disease, suggesting its role tumor expansion. this context, identification early biomarkers useful diagnosis cancer mandatory. Fibulin-3 represents an important marker However, it remains be determined...

Interleukin-6 (IL-6) is a pleiotropic cytokine involved in several mechanisms, and the alteration of IL-6 signaling leads to overactivation various processes including immunity, inflammation, hemostasis. Although increase has been documented venous thromboembolic diseases, exact involvement deep vein thrombosis (DVT) not fully understood. Consequently, we investigated trans-signaling inflammatory events occurring DVT, focusing on role interleukin-6 receptor (IL6-R) Asp358Ala variant. The...

Neutrophil gelatinase-associated lipocalin (NGAL), matrix metalloproteinase (MMP)-9, and NGAL/MMP-9 complex have been evaluated as diagnostic markers of several cancers, but results for bladder cancer are scanty. We these proteins in urine serum 89 patients with histologically confirmed 119 cancer-free controls from a case-control study. Urinary concentrations were standardized on creatinine level. The performance biomarkers was through the receiver operating characteristic (ROC) analysis....

Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease. Patients with RA have an increased risk for the development of cardiovascular diseases, however, pathophysiological mechanisms arterial complications in remain to be fully elucidated. Understanding early markers vascular damage may aid preventing patients RA. The current study investigated this by recruiting 30 and healthy subjects. Intima‑media thickness (IMT) was used detect presence atherosclerotic disease measured...

Background: Methicillin-resistant Staphylococcus aureus (MRSA) with intermediate resistance to Vancomycin (VISA) is reported worldwide. These strains frequently emerge among hospital-associated (HA)-MRSA and rarely within community-acquired (CA)-MRSA. Here, the genomic transcriptomic adaptations distinguishing VISA daptomycin resistant (DAP-R) CA-MRSA, which emerged in a hospitalized patient under glycopeptide treatment, were explored. Methods: Whole-genome sequencing, RNA-Seq bioinformatics...

Inflammation is a protective response of the body to various injuries, which strictly regulated by variety factors, including immune cells and soluble mediators. However, dysfunction this defensive mechanism often results in inflammation‑driven diseases, such as deep vein thrombosis (DVT). The complex relationship between inflammatory cell activity DVT has not been fully elucidated. present study aimed investigate role interleukin‑6 (IL6) signaling transduction DVT. To aim, expression levels...

8500 Background: Mutations in NRAS and BRAF are common cutaneous melanoma leading to constitutive activation of the MAPK pathway controlling cell proliferation. We examined effect mutations on pathologic features clinical outcomes patients with when compared tumors containing those wild-type (WT) for both. Methods: Clinical outcome data was obtained from a prospective cohort study 251 Australian centers. exon 2 (N61R) 15 were detected by high resolution melting sequence verified. Cox...

3533 Background: Cetuximab has proven efficacy in advanced CRC. However,the activity of cetuximab monotherapy is modest,hence further approaches are required. The combination (C) and erlotinib (E) shown synergistic preclinical models. This phase II study evaluated the C E patients with Methods: Eligibility included:metastatic CRC, PS 0-1, failure prior fluoropyrimidine, oxaliplatin irinotecan, but no previous EGFR targeted therapy, measurable disease, adequate organ function informed...

Abstract Malignant melanoma is an aggressive tumor of the skin with a poor prognosis for patients advanced disease. In melanoma, both Ras/Raf/MEK/ERK (MAPK) and PI3K/AKT (AKT) signaling pathways are constitutively activated through multiple mechanisms. Although, at present it has been demonstrated that treatment B-Raf enzyme inhibitors improve survival patients, still these may relapse or become resistant to treatment. Therefore, there need identify novel factors involved in development...

10610 Background: The PI3K/AKT/mTOR signaling pathway is a central network in number of cancers, including EOC. Identifying associations between key molecules and clinical outcomes may help expose develop therapeutic targets this cancer. Methods: Clinical data, tissue microarrays (TMAs) genomic DNA from 194 EOCs (100 serous, 58 endometrioid, 34 clear cell) were obtained the Australian Ovarian Cancer Study. These analyzed for mutations PIK3CA, AKT1, KRAS BRAF using high resolution melt...