A5053320317- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Innovative Microfluidic and Catalytic Techniques Innovation
- SARS-CoV-2 detection and testing
- Oral Health Pathology and Treatment
- Venous Thromboembolism Diagnosis and Management
- BRCA gene mutations in cancer
- Peptidase Inhibition and Analysis
- Cancer Genomics and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Biosensors and Analytical Detection
- Protease and Inhibitor Mechanisms
- Sexual function and dysfunction studies
- Retinal Development and Disorders
- Oral and gingival health research
- Probiotics and Fermented Foods
- Histone Deacetylase Inhibitors Research
- Oral health in cancer treatment
- Inflammasome and immune disorders
- Renal and related cancers
- Phytochemistry and Bioactive Compounds
- RNA Interference and Gene Delivery
University of Catania
2019-2025
Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) is the gold standard method for diagnosis of COVID‑19 infection. Due to pre‑analytical and technical limitations, samples with low viral load are often misdiagnosed as false‑negative samples. Therefore, it important evaluate other strategies able overcome limits RT‑qPCR. Blinded swab from two individuals diagnosed positive negative were analyzed by droplet digital PCR (ddPCR) RT‑qPCR in order assess sensitivity both...
There is recent evidence to indicate the existence of an inverse association between incidence neurological disorders and cancer development. Concurrently, transcriptional pathways responsible for onset glioblastoma multiforme (GBM) Alzheimer's disease (AD) have been found be mutually exclusive two pathologies. Despite advancements being made concerning knowledge molecular mechanisms development GBM AD, little known about identity microRNA (miRNAs or miRs) involved in progression these...
Glioblastoma multiforme (GBM) is the most frequent and deadly human brain cancer. Early diagnosis through non-invasive biomarkers may render GBM more easily treatable, improving prognosis of this currently incurable disease. We suggest use serum extracellular vesicle (sEV)-derived circular RNAs (circRNAs) as highly stable minimally invasive diagnostic for diagnosis. EVs were isolated by size exclusion chromatography from sera 23 5 grade 3 glioma (GIII) patients, 10 unaffected controls (UC)....
Abstract Background Gut microbiota modulation has been demonstrated to be effective in protecting patients against detrimental effects of anti-cancer therapies, as well improve the efficacy certain treatments. Among most characterized probiotics, Lactobacillus rhamnosus GG (LGG) is currently utilized clinics alleviate diarrhea, mucositis or intestinal damage which might associated with several triggers, including Clostridium difficile infections, inflammatory gut diseases, antibiotic...
Environmental or occupational exposure to pesticides is considered one of the main risk factors for development various diseases. Behind pesticide-associated pathologies, there are both genetic and epigenetic alterations, where these latter mainly represented by alteration in expression levels microRNAs change methylation status DNA. At present, no studies have comprehensively evaluated alterations induced pesticides; therefore, aim present study was identify modifications gene miRNA DNA...
Pomegranate is an important source of several bioactive compounds with beneficial properties. As widely described in the literature, pomegranate can be considered as a functional food able to promote maintenance good health status. The present review article summarizes properties pomegranate, such antioxidant, anti‑inflammatory, anti‑aging, prebiotic and anticancer effects, well its protective role against metabolic disorders cardiovascular diseases. phytocompounds prevent development...
IL-6 pathway is abnormally hyperactivated in several cancers triggering tumor cell growth and immune system inhibition. Along with genomic mutation, the IL6 gene expression can be affected by DNA methylation, microRNAs, post-translational modifications. Computational analysis was performed on Cancer Genome Atlas (TCGA) datasets to explore role of IL6, IL6R, IL6ST, IL6R transmembrane isoform their epigenetic regulation different cancer types. significantly modulated 70% types, revealing...
Breast and ovarian cancer represent two of the most common tumor types in females worldwide. Over years, several non‑modifiable modifiable risk factors have been associated with onset progression these tumors, including age, reproductive factors, ethnicity, socioeconomic status lifestyle as well family history genetic factors. Of note, BRCA1 BRCA2 are suppressor genes a key role DNA repair processes, whose mutations may induce genomic instability increase development. Specifically, breast or...
High-grade gliomas are the most fatal brain tumors. Grade 4 called glioblastoma multiforme (GBM), which associated with poorest survival and a 5-year rate of less than 4%. Many patients GBM developed concomitant cognitive dysfunctions epilepsy. Although decline is well defined in glioblastomas, neurotoxic factors underlying this pathology not understood patients. In study, we aimed to investigate whether GBM-derived exosomes play role neuronal toxicity. For purpose, obtained from T98G U373...
Colorectal cancer (CRC) is the fourth most commonly diagnosed and one of leading causes death worldwide. Despite diagnostic therapeutic advances, CRC mortality remains high, especially in industrialized countries. Numerous studies have highlighted pathogenetic role altered microRNA (miRNA) expression among various factors contributing to development progression colorectal (CRC). However, data regarding specific miRNAs involved pathogenesis remain inconsistent, no been recognized so far as...
Despite the availability of screening programs, oral cancer deaths are increasing due to lack diagnostic biomarkers leading late diagnosis and a poor prognosis. Therefore, there is an urgent need discover novel effective for this tumor. On these bases, aim study was validate potential microRNAs (miRNAs) through analysis liquid biopsy samples obtained from ten patients healthy controls. The expression four selected miRNAs evaluated by using droplet digital PCR (ddPCR) in pilot cohort donors....
DNA methylation is an epigenetic modification that plays a key role in several cellular processes mediating the fine regulation of gene expression. Aberrant observed wide range pathologies, including cancer. Since these modifications are transferred to cell progenies and stable over time, analysis status has been proposed for diagnostic prognostic purposes Currently, bisulfite conversion gold standard method high‑throughput alterations. However, treatment induces fragmentation affecting its...
Oral lichen planus (OLP) is a chronic inflammatory autoimmune disease of the oral cavity with malignant potential affecting 1.01% worldwide population. The clinical patterns this disorder, characterized by relapses and remissions lesions, appear on buccal, lingual, gingival, labial mucosa causing significant reduction in quality life. Currently, there are no specific treatments for disease, available therapies topical systemic corticosteroids only reduce symptoms. Although etiopathogenesis...
Legionella pneumophila (L. pneumophila) is a harmful pathogen often found in water systems. In hospitals, the absence of L. systems mandatory by law, therefore, frequent and effective monitoring fundamental importance. Molecular methods based on reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) have been proposed for detection pneumophila, however, sensitivity accuracy these not validated yet. Therefore, it important to evaluate other strategies able overcome limits...
Several features of cancer cells such as proliferation, invasion, metastatic spreading, and drug resistance are affected by their interaction with several tumor microenvironment (TME) components, including neutrophil gelatinase-associated lipocalin (NGAL), solute carrier family 22 member 17 (SLC22A17), matrix metallopeptidase 9 (MMP9). These molecules play a key role in growth, iron-dependent metabolism cells. However, the precise epigenetic mechanisms underlying gene regulation Lipocalin 2...