A5059470940- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- Venomous Animal Envenomation and Studies
- Extracellular vesicles in disease
- Erythrocyte Function and Pathophysiology
- Chemokine receptors and signaling
- Immune Cell Function and Interaction
- Insect and Arachnid Ecology and Behavior
- Immune Response and Inflammation
- Circular RNAs in diseases
- Insect and Pesticide Research
- Acute Myocardial Infarction Research
- Milk Quality and Mastitis in Dairy Cows
- Agricultural Practices and Plant Genetics
- Advanced X-ray and CT Imaging
- Viral gastroenteritis research and epidemiology
- Animal Diversity and Health Studies
- Systemic Lupus Erythematosus Research
- Escherichia coli research studies
- Agricultural pest management studies
- RNA modifications and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chemical Thermodynamics and Molecular Structure
Al-Azhar University
2024
Purdue University West Lafayette
2018-2024
Indiana University
2024
Hamad Medical Corporation
2024
Zagazig University
2017-2021
Menoufia University
1988-2020
University of Cambridge
2020
Lighthouse Guild
2020
Georgia State University
2016-2018
Zero to Three
2018
Significance The present study reveals that macrophage phagocytosis toward healthy self-cells is controlled by a two-tier mechanism: forefront activation mechanism requiring the inflammatory cytokine-stimulated protein kinase C (PKC)-spleen tyrosine (Syk) pathway, to which IL-10 conversely regulates, and subsequent self-target discrimination CD47-signal regulatory α (SIRPα)–mediated inhibition. findings significantly expand our understanding of phagocytic plasticity behavior under different...
Altered by defects in p53, epigenetic silencing, and genomic loss, the microRNA miR-34a represents one of most clinically relevant tumor-suppressive microRNAs. Without question, a striking number patients with cancer would benefit from replacement, if poor stability, non-specific delivery, delivery-associated toxicity could be overcome. Here, we highlight fully modified version (FM-miR-34a) that overcomes these hurdles when conjugated to synthetically simplistic ligand. FM-miR-34a is orders...
Use of tumor-suppressive microRNAs (miRNAs) as anti-cancer agents is hindered by the lack effective delivery vehicles, entrapment miRNA within endocytic compartments, and rapid degradation nucleases. To address these issues, we developed a strategy that includes (1) targeting ligand, (2) an endosomal escape agent, nigericin (3) chemically modified miRNA. The DUPA (2-[3-(1,3-dicarboxy propyl) ureido] pentanedioic acid), was selected based on its specificity for prostate-specific membrane...
Prostate cancer (PCa) remains a common with high mortality in men due to its heterogeneity and the emergence of drug resistance. A critical factor contributing lethality is presence prostate stem cells (PCSCs), which can self-renew, long-term propagate tumors, mediate treatment MicroRNA-34a (miR-34a) has shown promise as an anti-PCSC therapeutic by targeting molecules involved cell (CSC) survival functions. Despite extensive efforts, development miR-34a therapeutics still faces challenges,...
Although previous reports suggest that tumor-induced myeloid-derived suppressor cells (MDSC) inhibit T by L-arginine depletion through arginase-1 activity, we herein show is neither inherently expressed in MDSC nor required for MDSC-mediated inhibition. Employing Percoll density gradients, large expansions of the bone marrow tumor-bearing mice were isolated and demonstrated potent inhibition T-cell proliferation activated TCR-ligation, Concanavalin A, PMA plus ionomycin, or IL-2. Despite...
Abstract Chronic diseases are often associated with altered inflammatory response, leading to increased host vulnerability new challenges. Employing streptozotocin (STZ)-induced diabetes as a model, we further investigate mechanisms enhanced neutrophil (polymorphonuclear leukocytes [PMN]) responses under hyperglycemia and compare them those chronic colitis. We show that, different from colitis which the PMN response is significantly potentiated, existence of proinflammatory state broad...
RNA interference (RNAi)-based therapeutics (miRNAs, siRNAs) have great potential for treating various human diseases through their ability to downregulate proteins associated with disease progression. However, the development of RNAi-based is limited by lack safe and specific delivery strategies. A effort has been made overcome some these challenges resulting in N-acetylgalactosamine (GalNAc) ligands that are being used siRNAs treatment affect liver. The successes achieved using...
The therapeutic promise of small-RNA therapeutics is limited, not only by the lack delivery vehicles, but also inability small RNAs to reach intracellular compartments where they can be biologically active. We previously reported successful functionally active miRNAs via receptor-mediated endocytosis. This type targeted therapy still faces a major challenge in field: endosomal sequestration. Here, new method has been developed promote escape delivered miRNA. strategy relies on difference...
Abstract Myeloid‐derived suppressor cells (MDSCs) promote tumor growth through, in part, inhibiting T‐cell immunity. However, mechanisms underlying MDSC expansion and guidance of MDSCs toward the microenvironment remain unclear. Employing Percoll density gradients, we separate bone marrow (BM) leukocytes from tumor‐bearing mice into four density‐increasing bands with myeloid enriched III IV. Band comprises monocytes low‐density granulocytes, both confirmed to be M‐MDSCs G‐MDSCs,...
Ornithine transcarbamylase (OTC) deficiency (OTCD) is an X-linked urea cycle disorder. Being disease, the onset and severity of disease may vary among female carriers. Some them start to develop early in life, whereas others remain asymptomatic throughout their lives. Our patient was a 42-year-old man who developed severe hyperammonemia fatal brain edema after receiving right lobe graft from living donor with unrecognized OTCD. The disturbed level consciousness that managed successfully by...
Aim: This study was designed for the 1st time to describe normal head structures of one-humped camel (Camelus dromedarius) using both magnetic resonance imaging (MRI) and computed tomography (CT) as well cross-sectional anatomy. Materials Methods: Five fresh cadaver heads were collected from clinically camels then subjected T1-weighted MR CT imaging. Afterward, these examined transversely sliced obtain seven crossing levels. Results: The obtained per each level matched with their relevant...
Abstract Tumor-induced myeloid derived suppressive cells (MDSC) have been reported to inhibit T cell responses. In our study MDSC proliferation through arginase-1 dependent manner. However, surprisingly we observe that freshly isolated from the bone marrow of tumor bearing mice do not constitutively express until after exposure system. The aim this is determine mechanism by which induced in following a proliferative environment. We show treatment with culture supernatant activated CD3/CD28...
Background: Miscarriage is spontaneous expulsion of product conception from the uterus before viability (20 weeks) or if fetus less than 500 gm, its length 25 cm.Bacterial vaginosis extremely prevalent condition, although it not a reportable disease.Up to 50% pregnant women have been found BV.Methods; In this study, we assessed 219 for association between bacterial and first trimester miscarriage by screening using Nugent gram stain scoring system among at 7 weeks gestation follow them up...
Abstract Cytokine signaling is critical for cell-to-cell communication in development, homeostasis and disease. Interleukin (IL)-9 promotes allergic diseases by acting on both immune structural cells. In hematopoietic cells, IL-9 receptor (IL-9R) consists of two subunits – the α-chain (IL-9Rα) a common γ (γc) chain. However, IL-9-responsive epithelial cells express IL-9Rα but not γc chain raising question how these respond to absence established IL-9R complex. Here, we discovered that airway...
IVIg is a blood-derived antibody product initially designed as replacement therapy in inborn errors of immunity (IEIs). However, over the last 50 years, has been used to treat growing range autoimmune, autoinflammatory, and secondary immunodeficiency disorders. The US FDA licensed for use treatment nine clinical indications; although, global usage extends off-label indications with variable responses. Data from Qatar on scarce; thus, hampering formulation local policies. This study aimed...
Abstract Prostate cancer (PCa) remains a common with high mortality in men due to its heterogeneity and the emergence of drug resistance. A critical factor contributing lethality is presence prostate stem cells (PCSCs), which can self-renew, long-term propagate tumors mediate treatment MicroRNA-34a (miR-34a) has shown promise as an anti-PCSC therapeutic by targeting molecules involved cell (CSC) survival functions. Despite extensive efforts, development miR-34a therapeutics still faces...
The density and microhardness of the system Se100−xSbx (where x=5, 10, 15 at. %) were determined at room temperature using stepwise method. values increase with time annealing, Sb content. energy required for crystallization processes was calculated from as 31.82, 39.8, 49.7 K cal/mol samples Se95Sb5, Se90Sb10, Se85Sb15, respectively. On other hand, these energies are given by 43.76, 49.72, 54.7 cal/mol, data. dimensional nature growth process has been looked through determination n exponent...