A5034002485- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Nanoparticles: synthesis and applications
- Lung Cancer Treatments and Mutations
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Food composition and properties
- Graphene and Nanomaterials Applications
- Proteins in Food Systems
- Air Quality and Health Impacts
- Pulmonary Hypertension Research and Treatments
- Advanced Drug Delivery Systems
- Circular RNAs in diseases
- Heavy Metal Exposure and Toxicity
- Microplastics and Plastic Pollution
- Anesthesia and Neurotoxicity Research
- Occupational and environmental lung diseases
- Occupational exposure and asthma
- Nanocomposite Films for Food Packaging
- CRISPR and Genetic Engineering
- Environmental Toxicology and Ecotoxicology
- Diet and metabolism studies
Purdue University West Lafayette
2019-2025
Center for Cancer Research
2019-2023
University of Massachusetts Lowell
2015-2020
Engineered nanomaterials are increasingly added to foods improve quality, safety, or nutrition. Here we report the ability of ingested nanocellulose (NC) materials reduce digestion and absorption fat. In small intestinal phase an acellular simulated gastrointestinal tract, hydrolysis free fatty acids (FFA) from triglycerides (TG) in a high-fat food model was reduced by 48.4% when NC at 0.75% w/w food, as quantified pH stat titration, 40.1% assessed fluorometric FFA assay. Furthermore,...
Engineered nanomaterials (ENM) are extensively used as food additives in numerous products, and at present, little is known about the fate of ingested ENM (iENM) gastrointestinal (GI) environment. Here, we investigated dissolution behavior, biodurability, persistence four major iENM (TiO2, SiO2, ZnO, two Fe2O3) individual simulated GI fluids (saliva, gastric, intestinal) a physiologically relevant digestion cascade (saliva → gastric fasted state over time frames. TiO2 was found to be most...
Altered by defects in p53, epigenetic silencing, and genomic loss, the microRNA miR-34a represents one of most clinically relevant tumor-suppressive microRNAs. Without question, a striking number patients with cancer would benefit from replacement, if poor stability, non-specific delivery, delivery-associated toxicity could be overcome. Here, we highlight fully modified version (FM-miR-34a) that overcomes these hurdles when conjugated to synthetically simplistic ligand. FM-miR-34a is orders...
Use of tumor-suppressive microRNAs (miRNAs) as anti-cancer agents is hindered by the lack effective delivery vehicles, entrapment miRNA within endocytic compartments, and rapid degradation nucleases. To address these issues, we developed a strategy that includes (1) targeting ligand, (2) an endosomal escape agent, nigericin (3) chemically modified miRNA. The DUPA (2-[3-(1,3-dicarboxy propyl) ureido] pentanedioic acid), was selected based on its specificity for prostate-specific membrane...
EGFR inhibitors (EGFRi) are standard-of-care treatments administered to patients with non-small cell lung cancer (NSCLC) that harbor alterations. However, development of resistance posttreatment remains a major challenge. Multiple mechanisms can promote survival EGFRi-treated NSCLC cells, including secondary mutations in and activation bypass tracks circumvent the requirement for signaling. Nevertheless, involved signaling understudied require further elucidation. In this study, we identify...
Extracellular vesicles (EVs) from various biofluids have demonstrated potential as prognostic and diagnostic biomarkers in several diseases. However, their utility early stages of disease development has not been extensively investigated. Additionally, given role intercellular communication, decoding the landscape underlying cell–to–cell signaling a clinical or subclinical condition based on EV-specific analyses so far remained challenging. Here, we demonstrate urinary EVs for cross-week...
Abstract Lung cancer remains the leading cause of cancer-related deaths worldwide, largely due to late diagnoses often coinciding with advanced, metastatic stages. Thus, a deeper understanding tumor microenvironment (TME) and molecular mechanisms driving progression metastasis is critical. Among many contributors TME, extracellular vesicles (EVs) are crucial in facilitating intercellular communication, enabling transport biomolecules such as lipids, RNAs, DNA, proteins. Of various...
Abstract Osteosarcoma is a common type of bone cancer primarily observed in children, teenagers, and young adults. Current treatments (including surgery, chemotherapy, radiotherapy) are invasive induce aggressive side effects. Alternative approaches such as using microRNAs (miRNA) emerging promising anticancer agents. miRNA modulates tumorigenesis via tumor-suppressive oncogenic miRNAs. Tumor-suppressive miRNAs respectively downregulate mRNA transcripts. Hence, leveraging the activity...
Abstract Prostate cancer is a leading cause of cancer-related mortality in men. Current therapeutic options include surgical intervention, chemotherapy, immunotherapy, and localized or systemic radiation therapy. However, advanced metastatic prostate cancers are challenging to treat, underscoring the critical need for more effective strategies. Prostate-specific membrane antigen (PSMA), transmembrane glycoprotein, overexpressed up 1000-fold ∼90% cancers. The high expression level PSMA,...
Immunotherapy has significantly improved the survival of patients with various malignancies. However, immunotherapies have largely remained ineffective in tumors that reduced infiltration T cells, also called immune-cold tumors. Despite an extensive understanding factors mediate poor cell infiltration, current therapies target these shown modest clinical benefit, indicating other contribute to this process. One such factor been immunosuppression are extracellular vesicles (EVs). due...
Abstract Extracellular vesicles (EVs) released from non-small cell lung cancer (NSCLC) cells are known to promote progression. However, it remains unclear how EVs various NSCLC differ in their secretion profile and ability phenotypic changes non-tumorigenic cells. Here, we performed a comparative analysis of EV release (HBEC/BEAS-2B) several lines (A549, H460, H358, SKMES, Calu6) evaluated the potential impact EVs, including EV-encapsulated RNA (EV-RNA), driving invasion epithelial barrier...
Zebrafish provides rich toxicity data and similar high throughput in comparison to vitro models, features that make it an attractive model for nanomaterial screening, especially when frameworks prefer minimize animal testing.
Photocopying in offices and printing centers releases nanoparticles that can reach the brain following inhalation. We examined whether subcytotoxic levels of airborne photocopy-emitted could potentiate perturbation synaptic signaling cultured neurons exposure to amyloi d-β (Aβ). Signaling was only transiently inhibited by Aβ or individually, but remained statistically reduced cultures receiving both after 24 h. In vitro vivo studies with copier emitted have consistently demonstrated...
Embryonic expression of DNMT3B is critical for establishing de novo DNA methylation. This study uncovers the mechanism through which promoter-associated long non-coding RNA (lncRNA) Dnmt3bas controls induction and alternative splicing Dnmt3b during embryonic stem cell (ESC) differentiation. recruits PRC2 (polycomb repressive complex 2) at cis-regulatory elements gene expressed a basal level. Correspondingly, knockdown enhances transcriptional induction, whereas overexpression dampens it....
<div>Abstract<p>EGFR inhibitors (EGFRi) are standard-of-care treatments administered to patients with non–small cell lung cancer (NSCLC) that harbor EGFR alterations. However, development of resistance posttreatment remains a major challenge. Multiple mechanisms can promote survival EGFRi-treated NSCLC cells, including secondary mutations in and activation bypass tracks circumvent the requirement for signaling. Nevertheless, involved signaling understudied require further...
<div>Abstract<p>EGFR inhibitors (EGFRi) are standard-of-care treatments administered to patients with non–small cell lung cancer (NSCLC) that harbor EGFR alterations. However, development of resistance posttreatment remains a major challenge. Multiple mechanisms can promote survival EGFRi-treated NSCLC cells, including secondary mutations in and activation bypass tracks circumvent the requirement for signaling. Nevertheless, involved signaling understudied require further...
Supplementary Data from Loss of KMT5C Promotes EGFR Inhibitor Resistance in NSCLC via LINC01510-Mediated Upregulation MET
Supplementary Data from Loss of KMT5C Promotes EGFR Inhibitor Resistance in NSCLC via LINC01510-Mediated Upregulation MET
Abstract Due to the pleiotropic nature of miR-34a, including its ability downregulate multiple genes that drive resistance currently used anti-cancer therapies, we hypothesize best use miR-34a in a clinical setting would be combination with standard-of-care treatments. For vivo use, such molecule necessitate following: i) stabilized version can withstand harsh environment circulation and intracellular nucleases, ii) robust, specific, safe delivery vehicle. With regard stability, successfully...
Abstract Extracellular vesicle (EV) mediated transfer of biologically functional molecules between cancer cells and the surrounding environment significantly impacts progression. Our previous study established that non-small cell lung (NSCLC) cell-derived EV-RNA is an important mediator this communication, since it augments invasive potential recipient non-tumorigenic (BEAS2Bs). This led us to determine cargo specific NSCLC EVs. RNA sequencing analysis revealed preferential enrichment...