A5081299691- RNA Interference and Gene Delivery
- RNA modifications and cancer
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Acute Lymphoblastic Leukemia research
- RNA Research and Splicing
- Circular RNAs in diseases
- Microbial Metabolic Engineering and Bioproduction
- Genetics and Neurodevelopmental Disorders
- Cellular transport and secretion
- Catalysis and Oxidation Reactions
- Ubiquitin and proteasome pathways
- MXene and MAX Phase Materials
- Plant nutrient uptake and metabolism
- Lipid Membrane Structure and Behavior
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Microbial Fuel Cells and Bioremediation
- Microbial Natural Products and Biosynthesis
- Microbial metabolism and enzyme function
- Molecular Biology Techniques and Applications
Boston Children's Hospital
2018-2025
Dartmouth College
2024-2025
University of Padua
2025
Harvard Affiliated Emergency Medicine Residency
2023
Harvard University
2019-2023
Harvard Stem Cell Institute
2023
VIB-KU Leuven Center for Cancer Biology
2019
Center for Human Genetics
2019
Purdue University West Lafayette
2014-2019
KU Leuven
2019
The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to inexpensively conduct various screening assays investigate cytotoxicity cell based studies (Vichai and Kirtikara, 2006). This method relies on the property of SRB, which binds stoichiometrically proteins under mild acidic conditions then can be extracted using basic conditions; thus, amount bound dye as a proxy for mass, extrapolated measure proliferation. protocol divided into four main steps: preparation...
Use of tumor-suppressive microRNAs (miRNAs) as anti-cancer agents is hindered by the lack effective delivery vehicles, entrapment miRNA within endocytic compartments, and rapid degradation nucleases. To address these issues, we developed a strategy that includes (1) targeting ligand, (2) an endosomal escape agent, nigericin (3) chemically modified miRNA. The DUPA (2-[3-(1,3-dicarboxy propyl) ureido] pentanedioic acid), was selected based on its specificity for prostate-specific membrane...
MicroRNAs are small RNAs that negatively regulate gene expression posttranscriptionally. Because changes in microRNA can promote or maintain disease states, microRNA-based therapeutics being evaluated extensively. Unfortunately, the therapeutic potential of replacement is limited by deficient delivery vehicles. In this work, microRNAs delivered absence a protective vehicle. The method relies on direct attachment to folate (FolamiR), which mediates conjugated into cells overexpress receptor....
Biomethanation is a crucial process occurring in natural and engineered systems which can reduce carbon dioxide to methane impacting the global cycle. However, little known about effect of on-and-off gaseous provision micronutrients on bioconversion. Here, anaerobic microbiomes underwent intermittent feeding with incremental starvations selective metal supplementation assess impact hydrogen availability microbial physiology. Resilience was tested under differential cultivations basal medium...
The therapeutic promise of small-RNA therapeutics is limited, not only by the lack delivery vehicles, but also inability small RNAs to reach intracellular compartments where they can be biologically active. We previously reported successful functionally active miRNAs via receptor-mediated endocytosis. This type targeted therapy still faces a major challenge in field: endosomal sequestration. Here, new method has been developed promote escape delivered miRNA. strategy relies on difference...
Although peptides, antibodies/antibody fragments, siRNAs, antisense DNAs, enzymes, and aptamers are all under development as possible therapeutic agents, the breadth of their applications has been severely compromised by inability to reach intracellular targets. Thus, while macromolecules can often enter cells receptor-mediated endocytosis, missions frequently fail due an escape entrapping endosomes. In this paper, we describe a general method for promoting release any biologic material from...
Efforts to search for better treatment options cancer have been a priority, and due these efforts, new alternative therapies emerged. For instance, clinically relevant tumor-suppressive microRNAs that target key oncogenic drivers identified as potential anti-cancer therapeutics. MicroRNAs are small non-coding RNAs negatively regulate gene expression at the posttranscriptional level. Aberrant microRNA expression, through misexpression of genes, can profound cellular effects leading variety...
Abstract The therapeutic promise of small RNA therapeutics (siRNAs, miRNAs) is not only limited by the lack delivery vehicles, but also inability RNAs to reach intracellular compartments where they can be biologically active. We previously reported successful functionally active miRNAs via receptor-mediated endocytosis 1 . This type targeted therapy still faces one major challenges in field, endosomal sequestration. Here, a new method has been developed promote escape delivered miRNA....
The cells in your body contain genes made of DNA. Genes store the genetic information passed on to you by parents. This serves as recipe make proteins, and proteins build, maintain, heal every tissue body. cellular machinery that makes reads this with help small molecules called transfer RNAs (tRNAs), which supply necessary building blocks correct order construct specific proteins. To function properly, tRNAs must fold into three-dimensional shape—a process requires tRNA be decorated...
Abstract Amplification of chromosomal material derived from 12q is pathognomonic in a subset soft tissue sarcomas (STS), including well-differentiated and dedifferentiated liposarcoma. However, it remains unclear which the amplified genes are oncogenic drivers, obstacles to rational therapeutic development. We defined minimal amplicon human liposarcoma, tested activity each recurrently gene accelerate onset AKT-induced STS genetically engineered zebrafish model disease. This revealed several...
Abstract MicroRNAs (miRNAs) are exceptional candidates for combating many diseases such as cancer; however, their therapeutic power is dwarfed by the lack of safe, robust, specific, and efficient delivery methods. In theory, miRNAs represent “poster child” therapeutics. Firstly, small, thus, easily penetrate dense architecture tumor microenvironment, which a challenge larger molecules or carrier vehicles. Secondarily, expressed endogenously, due to tremendous selective pressure that has been...