A5056674108- Plant biochemistry and biosynthesis
- Alkaloids: synthesis and pharmacology
- Traditional and Medicinal Uses of Annonaceae
- Bioactive Natural Diterpenoids Research
- Chemical synthesis and alkaloids
- Pharmacological Effects of Natural Compounds
- Essential Oils and Antimicrobial Activity
- Microbial Natural Products and Biosynthesis
- Antioxidant Activity and Oxidative Stress
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Berberine and alkaloids research
- Photosynthetic Processes and Mechanisms
- Piperaceae Chemical and Biological Studies
- Enzyme Structure and Function
- Ginger and Zingiberaceae research
- Biochemical Acid Research Studies
- Natural product bioactivities and synthesis
- Plant and animal studies
- Gut microbiota and health
- Ginseng Biological Effects and Applications
- Chromatography in Natural Products
- Plant Gene Expression Analysis
- Advancements in Transdermal Drug Delivery
- Toxin Mechanisms and Immunotoxins
Prince of Songkla University
2014-2024
Technical University of Munich
1999-2002
Martin Luther University Halle-Wittenberg
2000-2001
Ludwig-Maximilians-Universität München
1999
2 -C -methylerythritol 4-phosphate has been established recently as an intermediate of the deoxyxylulose phosphate pathway used for biosynthesis terpenoids in plants and many microorganisms. We show that enzyme isolated from cell extract Escherichia coli converts into 4-diphosphocytidyl-2 by reaction with CTP. The is specified hitherto unannotated ORF ygbP E. . cognate protein was obtained pure form a recombinant hyperexpression strain harboring plasmid gene under control T5 promoter lac...
In many microorganisms, the putative orthologs of Escherichia coli ygbB gene are tightly linked or fused to ygbP , which has been shown earlier be involved in terpenoid biosynthesis. The E. was expressed a recombinant strain and direct synthesis soluble, 17-kDa polypeptide. protein found convert 4-diphosphocytidyl-2C-methyl- d -erythritol 2-phosphate into 2C-methyl- 2,4-cyclodiphosphate CMP. structure reaction product established by NMR spectroscopy using 13 C-labeled substrate samples....
A comparative analysis of all published complete genomes indicated that the putative orthologs unannotated ychB gene Escherichia coli follow distribution dxs , dxr and ygbP genes, which have been shown to specify enzymes deoxyxylulose phosphate pathway terpenoid biosynthesis, thus suggesting hypothetical YchB protein also is involved in pathway. To test this hypothesis, E. was expressed a homologous host. The recombinant purified homogeneity phosphorylate 4-diphosphocytidyl-2C-methyl- d...
The objective of this study was to obtain data on the distribution alkaloids in kratom plants grown Thailand. Two collections were performed, covering southern, central, and northern regions Thailand different seasons. contents alkaloids, including mitragynine (MG), paynantheine (PAY), speciogynine (SG), determined using validated HPLC method. 134 samples first collection collected from Nam Phu subdistrict, Ban Na San, Surat Thani, Thailand, during June October 2019 January 2020. maximum...
CYP3A4-mediated metabolic conversion of mitragynine to 7-hydroxymitragynine (7OH) has been demonstrated in human liver microsomes, and rodents. Pharmacokinetics (PK) 7OH humans is still limited. We aimed examine the pharmacokinetics formation healthy volunteers. To elucidate involvement CYP3A4 formation, inhibition by itraconazole was implemented. Two study periods with PK alone period 1, followed 2 including pretreatment conducted. Freshly prepared kratom tea consisting 23.6 mg given...
A hypothetical gene with similarity to the ispD of Escherichia coli was cloned from Arabidopsis thaliana cDNA. The ORF 909 bp specifies a protein 302 amino acid residues. cognate chromosomal consists 2,071 and comprises 11 introns size range 78-202 bp. fragment comprising residues 76-302 expressed in recombinant E. strain. purified homogeneity shown catalyze formation 4-diphosphocytidyl-2C-methyl-d-erythritol 2C-methyl-d-erythritol 4-phosphate specific activity 67 micromol small middle...
The putative catalytic domain (residues 81–401) of a predicted tomato protein with similarity to 4-diphosphocytidyl-2- C -methyl- d -erythritol kinase Escherichia coli was expressed in recombinant E. strain. purified homogeneity and shown catalyze the phosphorylation position 2 hydroxy group at rate 33 μmol⋅mg −1 ⋅min . structure reaction product, 2-phosphate, established by NMR spectroscopy. Divalent metal ions, preferably Mg 2+ , are required for activity. Neither enzyme nor ortholog...
This study aimed to evaluate the antioxidative constituents of Mitragyna diversifolia (Wall. ex G. Don) Havil. (MD). Total phenolic, total condensed tannin, and flavonoid contents were determined. Antioxidant activities assessed using colorimetric assays. The results indicated that leaf crude extract (LCE) had highest compared extracts from branches, stem barks, fruits. capacity LCE exhibited IC50 values 459.19 ± 9.08 μg/ml for 2,2-diphenyl-1-picrylhydrazyl assay 150.93 7.59 lipid...
The putative catalytic domain of an open reading frame from Plasmodium falciparum with similarity to the ispF gene Escherichia coli specifying 2 C ‐methyl‐ d ‐erythritol 2,4‐cyclodiphosphate synthase was expressed in a recombinant E. strain. protein purified homogeneity and found catalyze formation 4‐diphosphocytidyl‐2 2‐phosphate at rate 4.3 µmol·mg −1 ·min . At lower rates, catalyzes 2‐phospho‐2 3,4‐cyclophosphate ‐erythritol. Divalent metal ions such as magnesium or manganese are required...
The objectives of the present study were to examine influence supplementation with dried kratom leaf (DKTL) on performance, hematology, and nitrogen balance in goats. Four 12-month-old male crossbred (Thai Native x Anglo Nubian) goats an initial body weight (BW) 24.63 ± 0.95 kg allocated randomly receive four different levels DKTL using a 4 × Latin square design. was added total mixed ration (TMR) diet doses 0, 2.22, 4.44, 6.66 g/day investigate treatment’s efficacy. high secondary...
1‐Deoxy‐ D ‐xylulose‐5‐phosphate is converted into 2‐ C ‐methyl‐ ‐erythritol‐4‐phosphate by the catalytic action of 1‐deoxy‐ reductoisomerase (Dxr protein) using NADPH as cofactor. The stereochemical features this reaction were investigated in vitro experiments with recombinant Dxr protein Escherichia coli (4 R )‐ or S )‐[4‐ 2 H 1 ]NADPH coenzyme. enzymatically formed was isolated and 1,2:3,4‐di‐ O ‐isopropylidene‐2‐ ‐erythritol; NMR spectroscopic investigation derivative indicated that only...
Abstract BACKGROUND Kratom ( Mitragyna speciosa ) has a long history of traditional use. It contains various alkaloids and polyphenols. The properties kratom's have been well‐documented. However, the property polyphenols in water‐soluble phase less frequently reported. This study assessed effects (kratom) extract (MSE) on gut microbiota their metabolite production fecal batch culture. RESULTS kratom (MSE0) after partial sugar removal (MSE50) both contained polyphenols, with total phenolic...
1‐deoxy‐ d ‐xylulose 5‐phosphate serves as a precursor for the biosynthesis of vitamins thiamine and pyridoxal formation isopentenyl pyrophosphate dimethylallyl via nonmevalonate pathway terpenoid biosynthesis. Earlier studies had shown that Escherichia coli incorporates unphosphorylated into side chain ubiquinones with high efficacy. We show ‐xylulokinase E. (EC 2.7.1.17) catalyzes phosphorylation at hydroxy group C‐5 rate 1.6 µmol·mg min −1 . This reaction constitutes potential salvage...
Abstract A lateral flow‐based immunochromatographic strip was developed for the rapid detection of mitragynine (MG), a dominant alkaloid found in leaves kratom. Monoclonal antibody (mAb) against MG (anti‐MG mAb) conjugated to colloidal gold and used as recognition probe. MG‐ovalbumin conjugate (MG‐OVA) goat anti‐mouse IgG were immobilized on produce test zone control zone, respectively. Based principle competitive assay, sample competed with MG‐OVA resident bind gold‐anti‐MG mAb, resulting...
2 C ‐Methyl‐ d ‐erythritol 2,4‐cyclodiphosphate was recently shown to be formed from ‐methyl‐ 4‐phosphate by the consecutive action of IspD, IspE, and IspF proteins in nonmevalonate pathway terpenoid biosynthesis. To complement previous work with radiolabelled precursors, we have now demonstrated that [U‐ 13 5 ]2 affords isolated chromoplasts Capsicum annuum Narcissus pseudonarcissus . Moreover, are efficiently convert as well into carotene precursor phytoene. The bulk kinetic data collected...
Black pepper, dried green fruit of Piper nigrum L., is a household spice most popular in the world. Piperine, pungency compound black proposed to partially arise from phenylpropanoid pathway. In biosynthesis piperine, 4-coumarate:CoA ligase (4CLs) must play pivotal role activating intermediate acids corresponding CoA thioesters serve as substrates. Based on transcriptome data, we isolated three P. 4CL isoforms (Pn4CL1, -2, and -3) unripe peppercorn. These Pn4CLs were expressed E. coli for...