Muhammad Rafiq

ORCID: 0000-0002-4719-8849
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Research Areas
  • Polar Research and Ecology
  • Atomic and Molecular Physics
  • Microbial Community Ecology and Physiology
  • Advanced Chemical Physics Studies
  • Biocrusts and Microbial Ecology
  • Mass Spectrometry Techniques and Applications
  • Metal Extraction and Bioleaching
  • Synthesis and biological activity
  • Cold Atom Physics and Bose-Einstein Condensates
  • Minerals Flotation and Separation Techniques
  • Spectroscopy and Laser Applications
  • Mine drainage and remediation techniques
  • Microbial Applications in Construction Materials
  • Extraction and Separation Processes
  • Bacteriophages and microbial interactions
  • Synthesis and Reactions of Organic Compounds
  • Phytochemistry and Bioactivity Studies
  • Building materials and conservation
  • Algal biology and biofuel production
  • Cryospheric studies and observations
  • Bacterial biofilms and quorum sensing
  • melanin and skin pigmentation
  • Natural Antidiabetic Agents Studies
  • Microbial Metabolism and Applications
  • Marine Sponges and Natural Products

Balochistan University of Information Technology, Engineering and Management Sciences
2019-2025

Abdul Wali Khan University Mardan
2017-2024

South China University of Technology
2024

Bahauddin Zakariya University
2022

Quaid-i-Azam University
2008-2021

University of Veterinary and Animal Sciences
2021

Bacha Khan University
2021

University of Bristol
2017-2020

Bacteria inhabiting non-polar glaciers are exposed to large variations in temperature, which significantly affects the fluidity of bacterial cell membranes. In order maintain normal functions membranes, psychrophilic bacteria adapt by changing composition membrane fatty acids. However, information on exact pattern adaptability low-temperature habitats is scarce. present study, 42 strains were isolated from Ghulmet, Ghulkin, and Hopar Hunza Valley Karakoram Mountain Range, Pakistan their acid...

10.3389/fmicb.2020.00824 article EN cc-by Frontiers in Microbiology 2020-05-14

Agriculture in high altitude regions is challenged by low temperatures, and the use of chemical fertilizers turned hazardous long run. In addition, cost-effective, eco-friendly biofertilizers developed globally are less effective cold due to temperature-sensitive mesophilic homologs. The current study evaluated potential a cold-adapted indole acetic acid (IAA)-producing bacterial consortium isolated from Ghulkin glacier, Hunza Valley, Pakistan, promote growth crop, wheat, under stress....

10.1002/jobm.70017 article EN Journal of Basic Microbiology 2025-03-20

ABSTRACT Backed by advancements in technologies like microbial sequencing, many studies indicate that the vaginal microbiome is a key marker of female reproductive health. However, further are still needed to investigate correlation between microbiota (VMB) and outcomes assisted technology (ART). Therefore, this study compared VMB two types infertile women undergoing vitro fertilization (IVF) with normal control during implantation window period investigated effects characteristics on IVF...

10.1128/spectrum.01255-24 article EN cc-by Microbiology Spectrum 2025-01-27

Cultureable bacterial diversity of previously unexplored Siachen glacier, Pakistan, was studied. Out 50 isolates 33 (66%) were Gram negative and 17 (34%) positive. About half the pigment producers able to grow at 4-37°C. 16S rRNA gene sequences revealed bacteria dominated by Proteobacteria (especially γ-proteobacteria β-proteobacteria) Flavobacteria. The genus Pseudomonas (51.51%, 17) dominant among γ- proteobacteria. β-proteobacteria constituted 4 (12.12%) Alcaligenes Janthinobacterium...

10.1371/journal.pone.0178180 article EN cc-by PLoS ONE 2017-07-26

Metagenomic approach permits us to obtain the latent resources from culturable and unculturable microorganisms in ecosystem. In this study, high-throughput sequencing was practiced comprehensively probe prokaryotic community within extreme acidic environment of Baiyin open-pit mine stope, which varied pH other physicochemical parameters. Bioinformatics analysis further accomplished process millions Illumina reads analyzed alpha beta diversities, profile different samples obtained stope....

10.1080/01490451.2018.1430873 article EN Geomicrobiology Journal 2018-03-01

In this study, the efficiency of stone media fixed biofilm reactor (FBR) and sand column filter (SCF) was checked for domestic sewage treatment university area. Sewage continuously recirculated through FBR during different time intervals 12, 24, 36, 48 h followed by a SCF. There reduction in odor, alkalinity, pH, turbidity, biochemical oxygen demand (BOD5), chemical (COD), total dissolved solids, suspended electrical conductivity, phosphates (PO4), sulfates , nitrate nitrite bacterial count,...

10.1080/19443994.2014.903521 article EN cc-by-nc-nd Desalination and Water Treatment 2014-04-15

Metallotolerant fungi are known for their ability to adapt high concentrations of heavy metals by producing extracellular metabolites. We isolated an acid-producing filamentous fungus, designated as strain FMS1, from metal-polluted soil and identified Aspergillus tubingensis sequence analysis internal transcribed spacer regions. Physicochemical conditions growth well maximum tolerance various was determined. The carbon source optimized yield organic acids that were finally characterized...

10.1080/01490451.2019.1701585 article EN Geomicrobiology Journal 2019-12-12

AimThiss study was conducted to investigate the possible role of a compatible solute from radio‐halophilic bacterium against desiccation and ultra‐violet radiation‐induced oxidative stress.

10.1111/jam.13903 article EN Journal of Applied Microbiology 2018-05-05

In this study, we isolated and characterized bacteria analyzed the geochemistry of unexplored non-polar Tirich Mir glacier, Chitral, Pakistan. The glacier is located in Hindu Kush mountains range with highest peak 7708 m. Three types samples, i.e. glacial ice, sediment, melt water were collected. geochemical analysis demonstrated a higher concentration heavy metals Fe+2, Mn+2, Cr+2, cations Na+2, Ca+2, Mg+2, anions SO4−2 Cl−1. While among dissolved free amino acids, tyrosine was detected...

10.1080/01490451.2018.1551947 article EN Geomicrobiology Journal 2019-02-15

Abstract The Hindu Kush mountains spread over Northern areas of Pakistan having hundreds glaciers representing a unique ecosystem driven by the specific geochemistry and climate. current study measured distribution culturable fungi in Tirich Mir glacier, range, potential these isolates to show antimicrobial activity produce biotechnologically important enzymes. Samples glacial ice, sediments, meltwater were collected from 46 fungal strains isolated characterized for identity biotechnological...

10.1002/jobm.201900608 article EN Journal of Basic Microbiology 2020-03-09

We present measurements of photoionization cross sections the $3p\phantom{\rule{0.2em}{0ex}}^{1}P$ and $3p\phantom{\rule{0.2em}{0ex}}^{3}P$ excited states helium, at threshold near-threshold region $(0--0.2\phantom{\rule{0.3em}{0ex}}\mathrm{Ry})$. The experiments have been performed using a dc glow discharge employed saturation technique to determine sections. A smooth frequency dependence section has observed for both in accordance theoretical calculations. measured values section, simple...

10.1103/physreva.74.022715 article EN Physical Review A 2006-08-15

The photoionization cross section from the 3s3p 1P1 excited state has been measured in energy region first ionization threshold up to 1.4 eV excess using a two-step and saturated technique conjunction with an atomic beam source time-of-flight mass spectrometer that enables separation of three stable isotopes magnesium on time axis. absolute value sections near 3s as 90 ± 16 Mb (at 354.5 nm ionizing wavelength) for dominating isotope (24Mg) whereas at peak 3p2 1S0 auto-ionizing resonance...

10.1088/0953-4075/40/12/006 article EN Journal of Physics B Atomic Molecular and Optical Physics 2007-06-05
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