A5103185669- Lipid Membrane Structure and Behavior
- Phagocytosis and Immune Regulation
- Nitric Oxide and Endothelin Effects
- RNA Interference and Gene Delivery
- Adipokines, Inflammation, and Metabolic Diseases
- Erythrocyte Function and Pathophysiology
- Adipose Tissue and Metabolism
- Neuroscience and Neuropharmacology Research
- Nanoplatforms for cancer theranostics
- Eicosanoids and Hypertension Pharmacology
- Nanoparticle-Based Drug Delivery
- Autophagy in Disease and Therapy
- Photodynamic Therapy Research Studies
- Blood properties and coagulation
- Aortic Disease and Treatment Approaches
- Glycosylation and Glycoproteins Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Aortic aneurysm repair treatments
- Neuroscience of respiration and sleep
- Histone Deacetylase Inhibitors Research
- GDF15 and Related Biomarkers
- Immune cells in cancer
- Infectious Aortic and Vascular Conditions
- Sphingolipid Metabolism and Signaling
- Extracellular vesicles in disease
University of Cincinnati
2007-2017
University of Cincinnati Medical Center
2008-2016
Cincinnati Children's Hospital Medical Center
2015
HemoShear (United States)
2015
The University of Texas Southwestern Medical Center
2015
Georgia Regents Medical Center
2015
Augusta University
2015
Wright State University
2005-2013
Victor (Japan)
2012
Instituto Multidisciplinario de Biología Celular
2001
Differentiation of preadipocytes into mature adipocytes capable efficiently storing lipids is an important regulatory mechanism in obesity. Here, we examined the involvement histone deacetylases (HDACs) and acetyltransferases (HATs) regulation adipogenesis. We find that among various members HDAC HAT families, only HDAC9 exhibited dramatic down-regulation preceding adipogenic differentiation. Preadipocytes from gene knock-out mice accelerated differentiation, whereas overexpression 3T3-L1...
A growing number of studies support an important contribution astrocytes to neurovascular coupling, i.e., the phenomenon by which variations in neuronal activity trigger localized changes blood flow that serve match metabolic demands neurons. However, since both constriction and dilations have been observed brain parenchymal arterioles upon astrocyte stimulation, specific influences these cells on vasculature remain unclear. Using acute slices, we present evidence showing degree rat cortical...
// Subrahmanya D. Vallabhapurapu 1 , Víctor M. Blanco Mahaboob K. Sulaiman Swarajya Lakshmi Zhengtao Chu 1,2 Robert S. Franco and Xiaoyang Qi Division of Hematology Oncology, Department Internal Medicine, University Cincinnati College Cincinnati, Ohio, USA 2 Divison Human Genetics, Correspondence to: Qi, email: Keywords : phosphatidylserine, surface exposure, cancer cell biomarker, flippase, calcium Received August 27, 2015 Accepted September 09, Published October Abstract Viable cells...
During chronic caloric excess, adipose tissue expands primarily by enlargement of individual adipocytes, which become stressed with lipid overloading, thereby contributing to obesity-related disease. Although contains numerous preadipocytes, differentiation into functionally competent adipocytes is insufficient accommodate the excess and prevent adipocyte overloading. We report for first time that a high-fat diet (HFD) impairs adipogenic differentiation, leading accumulation inefficiently...
Basal and activity-dependent cerebral blood flow changes are coordinated by the action of critical processes, including autoregulation, endothelial-mediated signaling, neurovascular coupling. The goal our study was to determine whether astrocytes contribute regulation parenchymal arteriole (PA) tone in response hemodynamic stimuli (pressure/flow). Cortical PA vascular responses astrocytic Ca 2+ dynamics were measured using an vitro rat/mouse brain slice model perfused/pressurized PAs;...
High-fat diet (HFD) promotes endothelial dysfunction and proinflammatory monocyte activation, which contribute to atherosclerosis in obesity. We investigated whether HFD also induces the of red blood cells (RBCs), serve as a reservoir for chemokines via binding Duffy antigen receptor (DARC).A 60% 12 weeks, produced only minor changes lipid profile C57/BL6 mice, markedly augmented levels chemoattractant protein-1 bound RBCs, turn stimulated macrophage migration through an monolayer. Levels...
Normal brain function requires proper supply of oxygen and glucose in a timely local manner. This is achieved through an orchestrated intercellular communication between neurones, astrocytes microvessels that results rapid restricted increase cerebral blood flow, process known as neurovascular coupling. Astrocytic end-feet make close contacts with neuronal synapses vessels and, given their ability to release vasoactive signals following activation, have been recognized key intermediaries the...
Background Recruitment of macrophage precursors to the adventitia plays a key role in pathogenesis abdominal aortic aneurysms ( AAA s), but molecular mechanisms remain undefined. The innate immune signaling molecule CD 14 was reported be upregulated adventitial macrophages murine model and monocytes cocultured with fibroblasts (AoAf) vitro , concurrent increased interleukin‐6 (IL‐6) expression. We hypothesized that crucial precursor recruitment early during formation. Methods Results −/−...
// Víctor M. Blanco 1 , Zhengtao Chu 1,2 Subrahmanya D. Vallabhapurapu Mahaboob K. Sulaiman Ady Kendler 3 Olivier Rixe 4 Ronald E. Warnick 5 Robert S. Franco and Xiaoyang Qi Division of Hematology Oncology, Department Internal Medicine, University Cincinnati College Cincinnati, Ohio 2 Human Genetics, Pediatrics, Children's Hospital Medical Center, Pathology Laboratory Hematology/Oncology, Georgia Regents University, GRU Cancer Augusta, Neurosurgery, Brain Tumor Mayfield Clinic,...
Oxidative stress plays a fundamental role in abdominal aortic aneurysm (AAA) formation. Activated polymorphonuclear leukocytes (or neutrophils) are associated with AAA and express myeloperoxidase (MPO), which promotes inflammation, matrix degradation, other pathological features of AAA, including enhanced oxidative through generation reactive oxygen species. Both plasma MPO levels elevated patients but the pathogenesis has, heretofore, never been investigated. Here, we show that gene...
Epoxyeicosatrienoic acids (EETs), synthesized and released by astrocytes in response to glutamate, are known play a pivotal role neurovascular coupling. In vascular smooth muscle cells (VSMC), EETs activate large-conductance, Ca(2+)-activated K(+) (BK) channels resulting hyperpolarization vasodilation. However, the functional mechanism of action for glial-derived still be determined. this study, we evaluated effect synthetic EET analog 11-nonyloxy-undec-8(Z)-enoic acid (NUD-GA) on outward...
Abstract Lung cancer is the deadliest type of for both men and women. In this study, we evaluate in vitro vivo efficacy a biotherapeutic agent composed lysosomal protein (Saposin C, SapC) phospholipid (dioleoylphosphatidylserine, DOPS), which can be assembled into nanovesicles (SapC–DOPS) with selective antitumor activity. SapC–DOPS targets phosphatidylserine, an anionic preferentially exposed surface cells tumor-associated vasculature. Because binding SapC to phosphatidylserine favored at...
// Víctor M. Blanco 1 , Zhengtao Chu 1, 2 Kathleen LaSance 3 Brian D. Gray 4 Koon Yan Pak Therese Rider 5 Kenneth Greis Xiaoyang Qi Division of Hematology-Oncology, Department Internal Medicine, University Cincinnati College Cincinnati, Ohio 45267, USA Human Genetics, Pediatrics, Children's Hospital Medical Center, 45229, Radiology, Molecular Targeting Technologies, Inc., West Chester, Pennsylvania 19380, Cancer Biology, Correspondence to: Qi, email: xiaoyang.qi@uc.edu Keywords:...
Glioblastoma multiforme (GBM) is the most common malignant primary brain tumor.Classified by World Health Organization (WHO) as grade IV astrocytoma, GBMs are extremely aggressive, almost always recur, and despite our best efforts, remain incurable.This review describes traditional treatment approaches that led to moderate successes in GBM patients, discusses standard imaging modalities, presents data supporting use of SapC-DOPS, a novel proteoliposomal formulation with tumoricidal activity,...
Trophic molecules are key regulators of survival, growth and differentiation neural cells. Neuronal cell type Neuro‐2a is a good model to study development modulating this process, retinoic acid (RA) neurotrophins (NGF, BDNF, NT‐3 NT‐4) have been shown be active in modulation. The purpose the present was functional analysis these trophic our short‐term bioassay cells, an immortalised murine neuroblastoma line. Through counting, image process arithmetic combination digital parameters treated...
We describe a multi-angle rotational optical imaging (MAROI) system for in vivo monitoring of physiopathological processes labeled with fluorescent marker. Mouse models (brain tumor and arthritis) were used to evaluate the usefulness this method. Saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles tagged CellVue Maroon (CVM) fluorophore administered intravenously. Animals then placed holder (MARS) system. Images acquired 10° steps over 380°. A rectangular region interest (ROI)...
High toxicity, morbidity and secondary malignancy render chemotherapy of neuroblastoma inefficient, prompting the search for novel compounds. Nanovesicles offer great promise in imaging treatment cancer. SapC-DOPS, a stable nanovesicle formed from lysosomal protein saposin C dioleoylphosphatidylserine possess strong affinity abundantly exposed surface phosphatidylserine on cancer cells. Here, we show that SapC-DOPS effectively targets suppresses growth elucidate molecular mechanism action...
Background: Increased blood levels of ammonia (NH3) and ammonium (NH4+), i.e. hyperammonemia, leads to cellular brain edema in humans with acute liver failure. The pathophysiology this is poorly understood. This partly due incomplete understanding the osmotic effects pair NH3/NH4+ at molecular levels. Cell exposure solutions containing elicits changes intracellular pH (pHi), which can turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss solutes...
A current hypothesis suggests that glutamate-induced Ca2+ changes in astrocytes leads to arachidonic acid mobilization and production release of epoxyeicosatrienoic acids (EETs), which diffuse smooth muscle cells mediate vasodilation through their action on Ca2+-activated K+ channels (BK channels). Here, we present an alternative where EETs’ the neurovascular response is initiated at level astrocytes, particular endfeet, resulting BK channel activation contributing K+-induced vasodilation....
INTRODUCTION: Glioblastoma Multiforme (GBM) is the most aggressive form of primary brain tumor with a median survival <15 months from diagnosis. Cancer-selective nanotherapeutic agents such as SapC-DOPS, which targets phosphatidylserine (PS) on cell surface, offer an alternative paradigm for treatment intracranial neoplasms due to their ability transverse BBB without altering it. We have previously shown strong association between surface PS levels and SapC-DOPS-induced killing cancer cells....
We describe a multi-angle rotational optical imaging (MAROI) system for in vivo monitoring of physiopathological processes labeled with fluorescent marker. Mouse models (brain tumor and arthritis) were used to evaluate the usefulness this method. Saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles tagged CellVue Maroon (CVM) fluorophore administered intravenously. Animals then placed holder (MARS) system. Images acquired 10° steps over 380°. A rectangular region interest (ROI)...
SapC-DOPS nanovesicles are a stable protein-lipid mixture assembled from saposin C (SapC) and dioleylphosphatidylserine (DOPS). It shows selective tumor-targeting activity in multiple animal tumor models specific induction of apoptosis various cancer cell lines. The preferential targeting is due to higher levels exposed phosphatidylserine on the outer membrane cells. Importantly, by was shown occur through intracellular ceramide elevation. Ceramide known be suppressor molecule capable acting...