This study evaluated the depth of cure (DOC) packable and flowable bulk-fill resin-based composites (RBCs) including PRG (prereacted glass ionomer) short-fiber materials. The materials were placed in a black split-mold with 7 mm deep recess cured at 700 mW/cm2 for 20 s using LED curing light. DOC was assessed ISO scraping Knoops hardness tests. Data (n=5) computed analyzed one-way ANOVA/Scheffe's post hoc test (p<0.05). ranged from 3.66 to 2.54 while based on testing 3 1.5 mm. For all...

Injectable hydrogels have the great potential for clinical translation of dental pulp regeneration. A recently developed PEG-fibrinogen (PF) hydrogel, which comprises a bioactive fibrinogen backbone conjugated to polyethylene glycol (PEG) side chains, can be cross-linked after injection by photopolymerization. The objective this study was investigate use allows tuning its mechanical properties, as scaffold tissue engineering. cross-linking degree PF could controlled varying amounts...

BACKGROUND:The objective of this study was to investigate the accuracy 3-dimensional (3D) plastic (ABS) models generated using a low-cost 3D fused deposition modelling printer. MATERIAL AND METHODS:Two human dry mandibles were scanned with cone beam computed tomography (CBCT) Accuitomo device. Preprocessing consisted reconstruction Maxilim software and STL file repair Netfabb software. Then, data used print 2 replicas modeling printer (Up plus 2®). Two independent observers performed...

The tooth enamel organ (EO) is a complex epithelial cell assembly involved in multiple aspects of development, including amelogenesis. present study focuses on the role nonameloblast layers EO, stratum intermedium, stellate reticulum, and outer epithelium (OEE). secretory stage intermedium was distinguished by p63-positive stem marks, highly specific alkaline phosphatase labeling, as well desmosomes gap junctions. At location presecretory pre-eruption EO prominently featured papillary layer...

Inflammatory conditions affect periodontal ligament (PDL) homeostasis and diminish its regenerative capacity. The complexity of biological activities during an inflammatory response depends on genetic epigenetic mechanisms. To characterize the changes in to pathogens we have focused histone lysine methylation as a relatively stable chromatin modification involved activation repression transcription prime candidate mechanism responsible for exacerbated prolonged cells tissues dental plaque...

Microporous annealed particle (MAP) hydrogels are an emerging class of biomaterials with the potential to improve outcomes in tissue repair and regeneration. Here, a new MAP hydrogel platform comprising poly(ethylene) glycol (PEG) microparticles that situ using bio-orthogonal tetrazine click chemistry is reported (i.e., TzMAP hydrogels). Briefly, clickable PEG-peptide extracellular matrix mimetic peptides permit cell adhesion enzymatic degradation were fabricated via submerged...

The extracellular tooth enamel matrix is a unique, protein-rich environment that provides the structural basis for growth of long and parallel oriented crystals. Here we have conducted series in vivo vitro studies to characterize changes shape organization take place during transition from ameloblast intravesicular matrices subunit compartments pericrystalline sheath proteins, correlated these with stages amelogenin protein posttranslational processing. Our transmission electron microscopic...

There is growing concern that titanium particles may play a role in peri-implant breakdown. Ultrasonic scalers are routinely used the debridement of lesions. This vitro study designed to evaluate if produced when an ultrasonic scaler on implant.New sandblasted, large grit, acid etched (SLA) coated implants were subjected scaling with stainless steel, titanium, and PEEK plastic tips. The placed holding device was positioned SLA surface under 25 grams pressure. 30 motions. coolant water...

The loss of bone following tooth extraction poses a significant clinical problem for maxillofacial esthetics, function, and future implant placement. In the present study, efficacy an erythropoietin-impregnated collagen scaffold as alveolar ridge augmentation material versus conventional BioOss inorganic bovine xenograft was examined. collagen/Erythropoietin (EPO) exhibited significantly more rapid complete osseous regeneration defect when compared to membrane alone. new EPO induced...

YAP and TAZ are essential transcriptional co-activators downstream effectors of the Hippo pathway, regulating cell proliferation, organ growth, tissue homeostasis. To ask how pathway affects mineralized homeostasis in a that is highly reliant on tight homeostatic control deposition resorption, we determined effects YAP/TAZ dysregulation periodontal tissues alveolar bone, root cementum, ligament. Loss was associated with reduction density cellular cementum downregulation collagen I, alkaline...

The transport of mineral ions from the enamel organ-associated blood vessels to developing crystals involves complex cargo packaging and carriage mechanisms across several cell layers, including ameloblast layer stratum intermedium. Previous studies have established PHOSPHO1 as a matrix vesicle membrane-associated phosphatase that interacts with vesicles molecules phosphoethanolamine phosphocholine initiate apatite crystal formation inside in bone. In present study, we sought determine...

The nervous system exerts finely tuned control over all aspects of the life an organism, including pain, sensation, growth, and development. Recent developments in tissue regeneration research have increasingly turned to small molecule peptides tailor augment biological response following loss or injury. In present study, we introduced peptide galanin (GAL) as a novel scaffold-coating agent for healing craniofacial tissues. Using immunohistochemistry, detected GAL receptors healthy...

Tooth amelogenesis is a complex process beginning with enamel organ cell differentiation and matrix secretion, transitioning through changes in ameloblast polarity, cytoskeletal organization, affecting crucial biomineralization events such as mineral nucleation, crystal growth, prism organization. Here we have harvested the including pliable of postnatal first mandibular mouse molars during eight days tooth development to conduct step-wise cross-sectional analysis protein phase. Mineral...

The faithful engineering of complex human tissues such as the bone/soft tissue/mineralized tissue interface in periodontal requires innovative molecular cues conjunction with tailored scaffolds. To address loss bone and connective following disease, we have generated a polydopamine collagen coated electrospun PLGA-PCL (PP) scaffold enriched small molecule mediator PFI-2 (PP-PFI-pDA-COL-PFI). In vitro 3D studies using PDL progenitors revealed that PP-PFI-pDA-COL-PFI substantially enhanced...

The growth of long and polarized ameloblast-like cells has been heralded as a major prerequisite for enamel tissue engineering. In this study, we have designed three-dimensional bioreactor/scaffold microenvironments to propagate assess the ability cervical loop derivatives become cells. Our studies demonstrated that loop/periodontal progenitor coculture in growth-factor-enriched medium resulted formation expressing high levels amelogenin ameloblastin. Coculture with dental pulp on tailored...

Tooth enamel develops within a pH sensitive amelogenin-rich protein matrix. The purpose of the present study is to shed light on intimate relationship between matrix pH, self-assembly, and crystal growth during early amelogenesis. Universal indicator dye staining revealed highly acidic values (pH 3–4) at exocytosis site secretory ameloblasts. When increasing an amelogenin solution from 5 7, there was gradual increase in subunit compartment size 2 nm diameter subunits stretched configuration...

Gravity is one of the key determinants human cell function, proliferation, cytoskeletal architecture and orientation. Rotary bioreactor systems (RCCSs) mimic loss gravity as it occurs in space instead provide a microgravity environment through continuous rotation cultured cells or tissues. These RCCSs ensure an un-interrupted supply nutrients, growth transcription factors, oxygen, address some shortcomings gravitational forces motionless 2D (two dimensional) organ culture dishes. In present...

Gravity is one of the key determinants human cell function, proliferation, cytoskeletal architecture and orientation. Rotary bioreactor systems (RCCSs) mimic loss gravity as it occurs in space instead provide a microgravity environment through continuous rotation cultured cells or tissues. These RCCSs ensure an un-interrupted supply nutrients, growth transcription factors, oxygen, address some shortcomings gravitational forces motionless 2D (two dimensional) organ culture dishes. In present...