A5010489464- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Retinal and Optic Conditions
- Cardiac Ischemia and Reperfusion
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Imaging and Analysis
- Anesthesia and Neurotoxicity Research
- Glaucoma and retinal disorders
- Advanced Memory and Neural Computing
- Neurological Disorders and Treatments
- Immune Cell Function and Interaction
- Agriculture and Biological Studies
- Retinal and Macular Surgery
- Neuroscience and Neuropharmacology Research
- Drug-Induced Ocular Toxicity
- Hormonal Regulation and Hypertension
- Adolescent and Pediatric Healthcare
- Adrenal Hormones and Disorders
- Adenosine and Purinergic Signaling
- Ocular Disorders and Treatments
- Retinopathy of Prematurity Studies
- Phytoestrogen effects and research
- Laser Applications in Dentistry and Medicine
The University of Melbourne
2016-2024
Monash University
2008-2021
Australian Regenerative Medicine Institute
2021
Centre for Eye Research Australia
2016-2018
The University of Sydney
2013-2017
Robert Bosch (Australia)
2015-2017
Robert Bosch (India)
2015
Australian Synchrotron
2008
Abstract Age-related macular degeneration (AMD) is a leading cause of irreversible vision loss, characterised by the dysfunction and death photoreceptors retinal pigment epithelium (RPE). Innate immune cell activation accompanying para-inflammation have been suggested to contribute pathogenesis AMD, although exact mechanism(s) signalling pathways remain elusive. Pattern recognition receptors (PRRs) are essential activators innate system drivers para-inflammation. Of these PRRs, two most...
Synchrotron Fourier transform-infrared (FT-IR) and Raman microspectroscopy were applied to investigate changes in the molecular architecture of mouse oocytes demonstrate overall morphology maturing oocyte. Here we show that differences identified between immature at germinal vesicle (GV) mature metaphase II (MII) stage when using this technology, without introduction any extrinsic markers, labels, or dyes. GV found have a small, centrally located lipid deposit another larger polar similar...
Purpose: Following successful clinical outcomes of the prototype suprachoroidal retinal prosthesis, Bionic Vision Australia has developed an upgraded 44-channel prosthesis to provide a wider field view and more phosphenes. The aim was evaluate preclinical passive safety characteristics electrode array. Methods: Ten normal-sighted felines were unilaterally implanted with array containing platinum electrodes (44 stimulating 2 returns) on silicone carrier near area centralis. Clinical...
Purpose: This study explored whether the proangiogenic factor Angiotensin II (AngII) had a direct effect on activation state of microglia via type 1 receptor (AT1-R). Methods: Microglial dynamic activity was investigated in live retinal flatmounts from adult Cx3Cr1+/GFP mice under control, AngII (5 μM) or + candesartan (0.227 conditions. The effects intravitreal administration (10 mM) were also at 24 hours, with retinae processed for immunocytochemistry, flow cytometry, inflammatory...
Purpose: Simultaneous stimulation of multiple retinal electrodes in normally sighted animals shows promise improving the resolution prostheses. However, effects simultaneous on degenerate retinae remain unknown. Therefore, we investigated characteristics cortical responses to multielectrode retina. Methods: Four adult cats were bilaterally implanted with electrode arrays suprachoroidal space after unilateral adenosine triphosphate (ATP)–induced photoreceptor degeneration. Functional and...
Purpose: Subthreshold, nanosecond pulsed laser treatment shows promise as a for age-related macular degeneration (AMD); however, the safety profile needs to be robustly examined. The aim of this study was investigate effects in humans and mice. Methods: Patients with AMD were treated at subthreshold (no visible retinal effect) energy doses (0.15–0.45 mJ) sensitivity assessed microperimetry. Adult C57BL6J mice (0.065 suprathreshold (photoreceptor loss, 0.5 settings. vascular responses...
The ERG is the sum of all retinal activity. usually recorded from cornea, which acts as an antenna that collects and sums signals retina. a sensitive measure changes in function are pan-retinal, but less effective for detecting damage confined to small area In present work we describe how record 'flash' ERG, potential generated when retina exposed brief light flash. We methods anaesthesia, mydriasis corneal management during recording; keep dark adapted; electrode materials placement; range...
Purpose: To test whether remote ischemic preconditioning (RIP) is protective to photoreceptors, in a light damage model, and identify mechanisms involved. Methods: A pressure cuff was used induce ischemia (2 × 5 minutes) one hind limb of 4- 6-month-old albino Sprague-Dawley rats raised dim, cyclic (12 hours lux, 12 dark). Immediately following the ischemia, were exposed bright continuous (1000 lux) for 24 hours. After 7-day survival conditions, retinal function assessed using flash...
The pathogenesis of outer retinal degenerations has been linked to the elevation cytokines that orchestrate pro-inflammatory responses within milieu, and which are thought play a role in diseases such as geographic atrophy (GA), an advanced form AMD. Here we sought investigate anti-inflammatory mechanistic properties fludrocortisone (FA), well triamcinolone acetonide (TA), on Müller cell-mediated cytokine expression response inflammatory challenge. In addition, investigated neuroprotective...
Abstract Neovascular AMD (nAMD) leads to vision loss and is a leading cause of visual impairment in the industrialised world. Current treatments that target blood vessel growth have not been able treat subretinal fibrosis nAMD patients continue lose vision. The molecular mechanisms involved development fibrotic lesions are well understood. aim this study was further understand laser photocoagulation model choroidal neovascularization (CNV) by studying whole transcriptome RPE/choroid...
Unconventional T cells, including mucosal-associated invariant (MAIT), natural killer (NKT), and gamma-delta (γδT) comprise distinct T-bet+, IFN-γ+ RORγt+, IL-17+ subsets which play differential roles in health disease. NKT1 cells are susceptible to ARTC2-mediated P2X7 receptor (P2RX7) activation, but the effects on other unconventional T-cell types unknown. Here, we show that MAIT, γδT, NKT express P2RX7 sensitive P2RX7-mediated cell death. Mouse peripheral T-bet+ MAIT1, γδT1, especially...
To identify Myo/Nog cells in the adult retina and test their role protecting retinal photoreceptors from light damage.Light damage was induced by exposing albino rats raised dim cyclic to 1000 lux for 24 hours. In one group of rats, were purified rat brain tissue magnetic cell sorting following binding G8 monoclonal antibody (mAb). These injected into vitreous humour eye within 2 hours bright exposure. Retinal function assessed using full-field, flash electroretinogram (ERG) before after...
Remote ischemic preconditioning (RIP) has been found to be protective of heart and brain against injury. We have tested the effects RIP on retinal function using electroretinogram.Ischemia remote from retina was induced in one hindlimb, a pressure cuff applied for between 5 10 minutes. A temperature probe footpad confirmed blockage circulation. To test impact function, we recorded dark-adapted flash electroretinogram (ERG) four groups (n = per group) Sprague-Dawley rats (sham, 5-minute,...
Sublethal ischemia protects tissues against subsequent, more severe through the upregulation of endogenous mechanisms in affected tissue. has also been shown to upregulate protective remote tissues. A brief period (5-10 min) hind limb mammals induces self-protective responses brain, lung, heart and retina. The effect is known as ischemic preconditioning (RIP). It a therapeutically promising way protecting vital organs, already under clinical trials for brain injuries. This publication...
The majority of preclinical studies investigating multi-electrode field shaping stimulation strategies for retinal prostheses, have been conducted in normally-sighted animals. This study aimed to reassess the effectiveness two electrical techniques that shown work healthy retinae, a more clinically relevant animal model photoreceptor degeneration. Four cats were unilaterally blinded via intravitreal injections adenosine triphosphate. Cortical responses traditional monopolar (MP) stimulation,...
The ERG is the sum of all retinal activity. usually recorded from cornea, which acts as an antenna that collects and sums signals retina. a sensitive measure changes in function are pan-retinal, but less effective for detecting damage confined to small area In present work we describe how record 'flash' ERG, potential generated when retina exposed brief light flash. We methods anaesthesia, mydriasis corneal management during recording; keep dark adapted; electrode materials placement; range...
Abstract Unconventional T cells, including mucosal-associated invariant (MAIT), natural killer (NKT), and gamma-delta (γδT) comprise distinct T-bet + , IFN-γ RORγt IL-17 subsets which play differential roles in health disease. NKT1 cells are susceptible to ARTC2-mediated P2X7 receptor (P2RX7) activation, but the effects on other unconventional T-cell types unknown. Here, we show that MAIT, γδT, NKT express P2RX7 sensitive P2RX7-mediated cell death. Mouse peripheral MAIT1, γδT1, especially...