Transgenic soybean event CAL-16 expressing fusion Bt protein Cry1Ab-Vip3A was developed for conferring broad-spectrum resistance to lepidopteran pests Lepidopteran insect species are important causing significant yield loss and quality degradation worldwide. soybeans crystal (Cry) insecticidal proteins have been utilized pest management. However, the efficacy of currently adopted insect-resistant is challenged by evolution. Vegetative (Vips) highly active against a broad spectrum insects....

Plant transgenic technology has been widely utilized for engineering crops trait improvements and production of high value proteins such as pharmaceuticals. However, the unintended spreading commercial by pollination seed dispersal is a major concern environmental food safety. Simple reliable containment strategies transgenes are highly desirable. Here we report novel method creating selectively terminable rice. In this method, gene(s) interest tagged with RNA interference cassette, which...

Abstract Management of resistance development insect pests is great importance for continued utilization Bt crop. The high-dose/refuge and pyramid (gene stacking) strategy are commonly employed to delay the evolution resistance. Due anticipated difficulty deployment mandatory refuge transgenic crops in China, where size farmer quite small, stacking genes with different modes action a more feasible strategy. Here we report rice expressing fusion protein Cry1Ab Vip3A toxin. Analysis trypsin...

Genetically modified (GM) crops tolerant to glyphosate have delivered significant economic benefits in farm management. However, the evolution of resistance weeds due prolonged intensive use poses a serious threat this weed management system. It is highly desirable China deploy dual herbicide-tolerant corn at very beginning GM release delay development herbicides. Here, we report creation and characterization event SCB-29 that expresses both cp4 epsps bar genes. This transgenic maize up 3600...

Bermuda grass (Cynodon dactylon) is notoriously difficult to control with some commonly used herbicides. We cloned a cytochrome P450 gene from grass, named P450-N-Z1, which was found confer tolerance multiple herbicides in transgenic Arabidopsis. These include: (1) acetolactate synthase (ALS) inhibitor nicosulfuron and penoxsulam; (2) p-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicide mesotrione; (3) synthetic auxin dicamba; (4) photosynthesis bentazon. further generated soybean...

Transgene spreading is a major concern in cultivating genetically modified (GM) corn. Cross-pollination may cause the spread of transgenes from GM cornfields to conventional fields. Occasionally, seed lot contamination, volunteers, mixing during sowing, harvest, and trade can also lead transgene escape. Obviously, new biological confinement technologies are highly desired mitigate addition physical separation isolation methods. In this study, we report development built-in containment method...

Bacillus sp. A4 exhibiting laccase production was isolated from forest soil. Its secreted into a LB medium exhibited maximum activity of 3.9 U mg-1 protein at the optimal temperature (37 °C) and pH (6.0). The purified demonstrated low molecular mass 33 kDa, its were 40 °C 4.6, respectively, when using ABTS as substrate. significantly increased in presence Cu2+, methanol, ethanol, but it totally inhibited by L-cysteine. this strain markedly stimulated incubated with 0.5% different...

Background The major challenge of cultivating genetically modified (GM) rice (Oryza sativa) at the commercial scale is to prevent spread transgenes from GM cultivated its coexisting weedy (O. sativa f. spontanea). strategic development with a built-in control mechanism can mitigate transgene in populations. Methodology/Principal Findings An RNAi cassette suppressing expression bentazon detoxifying enzyme CYP81A6 was constructed into T-DNA which contained two tightly linked expressing Bt...

Background: Polygalacturonase is an important pectin degrading enzyme. The western honey bee (Apis mellifera L.) collects pollens from different flowers which are rich source of pectin. microbiota in the gut bee, release polygalacturonase enzyme and help digestion. This study aims to isolate characterize novel producing bacterial strain bee’s gut. Methods: was isolated by using agar plate assay. identified on basis morphology 16S rDNA sequence analysis. assay performed as a substrate....

Recently, controlled low-strength material (CLSM) has been considered an easy-to-mix material, and the raw is usually derived from solid waste, suggesting lower production costs. Moreover, resource utilization of waste fosters sustainable advancement both society environment. In present work, a CLSM with excellent performance was developed by adopting fly ash, bottom desulfuration gypsum, cement as main cementitious materials, well gasification coarse slag coal gangue aggregates. An...

The double-spotted leaf beetle (DLB), Monolepta hieroglyphica, is becoming a significant corn pest in China. It mainly attacks silk and developing kernels during the adult stage causing yield loss north-eastern damage caused by DLB expected to worsen as pesticide usage likely decrease along with upcoming commercial planting of transgenic lepidopteran-resistant maize Therefore, it highly desirable develop for resistance.

Abstract Phytase is one of the most effective feed additives to increase availability phosphorus and minerals by catalyzing hydrolysis phytic acid. A modified appA gene ( mappA ) was transformed into soybean Glycine max under control a seed-specific promoter from common bean Phaselous vulgaris ). The recombinant phytase showed optimal activity at pH 4.5 70 °C. slight in enzyme occurred when pre-incubated with n -hexane. In addition, our transgenic does not reduce even after 2 hours...

The helmet is a mandatory tool for safe production, and bamboo helmets can be used as an alternative to the traditional plastic safety helmet. In this study, were modified with urea-formaldehyde resin have shown excellent impact resistance, good color sensitivity, high gloss. performance of comes from structure designed by imitating gradient characteristics modification resin. pores defects in are filled repaired. chemical combination reduces bamboo’s crystallinity improves helmet’s...