A5047222507- Immune cells in cancer
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Carbohydrate Chemistry and Synthesis
- Allergic Rhinitis and Sensitization
- Respiratory Support and Mechanisms
- Immune Response and Inflammation
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Antifungal resistance and susceptibility
- Adenosine and Purinergic Signaling
- Clinical Nutrition and Gastroenterology
- NF-κB Signaling Pathways
- Food Allergy and Anaphylaxis Research
- RNA modifications and cancer
- Retinoids in leukemia and cellular processes
- Marine animal studies overview
- Heme Oxygenase-1 and Carbon Monoxide
- Sepsis Diagnosis and Treatment
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Fungal Infections and Studies
- Autoimmune Bullous Skin Diseases
- Acute Myeloid Leukemia Research
- Synthesis of Organic Compounds
University of Colorado Anschutz Medical Campus
2023
Children's Hospital Colorado
2023
University of Colorado Denver
2023
Washington University in St. Louis
2020
University of California, San Francisco
2015
University of Wisconsin–Madison
2010-2013
Université du Québec à Montréal
2012
Boston Children's Hospital
2006
Office of Infectious Diseases
2004
Loma Linda University
2001-2004
Newborns are at increased risk of overwhelming infection, yet the mechanisms underlying this susceptibility incompletely defined. In study we report a striking 1- to 3-log decrease in sensitivity monocytes human neonatal cord blood, compared with adult peripheral TNF-alpha-inducing effect multiple TLR ligands, including bacterial lipopeptides (BLPs), LPS, and imidazoquinoline compound, imiquimod. marked contrast, TNF-alpha release response R-848, ligand that is congener imiquimod, was...
Human newborns are susceptible to microbial infection and mount poor vaccine responses, yet the mechanisms underlying their susceptibility incompletely defined. We have previously reported that despite normal basal expression of TLRs associated signaling intermediates, human neonatal cord blood monocytes demonstrate severe impairment in TNF-alpha production response triacylated (TLR 2/1) diacylated 2/6) bacterial lipopeptides (BLPs). now marked contrast, BLP-induced synthesis IL-6, a...
Cellular integrins were identified as human cytomegalovirus (HCMV) entry receptors and signaling mediators in both fibroblasts endothelial cells. The goal of these studies was to determine the mechanism by which HCMV binds cellular mediate virus entry. envelope glycoprotein B (gB) has sequence similarity integrin-binding disintegrin-like domain found ADAM (a disintegrin metalloprotease) family proteins. To test ability this region bind integrins, we generated a recombinant soluble version gB...
Chitin exposure in the lung induces eosinophilia and alternative activation of macrophages is correlated with allergic airway disease. However, mechanism underlying chitin-induced polarization poorly understood. In this paper, we show that chitin vivo but does not do so directly vitro. We further epithelial cells bind vitro produce CCL2 response to both vivo. Supernatants chitin-exposed promoted vitro, whereas Ab neutralization supernate abolished macrophages. acted redundantly vivo, mice...
Blastomyces adhesin-1 (BAD-1) is a 120-kD surface protein on B. dermatitidis yeast. We show here that BAD-1 contains 41 tandem repeats and deleting even half of them impairs fungal pathogenicity. According to NMR, the form tightly folded 17-amino acid loops constrained by disulfide bond linking conserved cysteines. Each loop highly WxxWxxW motif found in thrombospondin-1 (TSP-1) type 1 heparin-binding repeats. binds heparin specifically saturably, competitively inhibited soluble heparin, but...
Abstract Alveolar inflammation is a hallmark of acute lung injury (ALI), and its clinical correlate respiratory distress syndrome—and it as result interactions between alveolar type II cells (ATII) macrophages (AM). In the setting injury, microenvironment intra‐alveolar space determined in part by metabolites cytokines known to shape AM phenotype. response ALI, increased glycolysis observed AT cells, mediated transcription factor hypoxia‐inducible (HIF) 1α, which has been shown decrease...
ABSTRACT Levels of the anaphylatoxin C3a are increased in patients with asthma compared those nonasthmatics and increase further still during exacerbations. However, role sensitization to allergen is poorly understood. Sensitization fungal allergens, such as Aspergillus fumigatus , a strong risk factor for development asthma. Exposure chitin, structural polysaccharide cell wall, induces innate allergic inflammation may promote allergens. Here, we found that coincubation chitin serum or...
Acute promyelocytic leukemia is an aggressive malignancy characterized by the accumulation of promyelocytes in bone marrow. PML/RARA primary abnormality implicated this pathology, but mechanisms which chimeric fusion protein initiates disease are incompletely understood. Identifying targets vivo critical for comprehending road to pathogenesis. Utilizing a novel sorting strategy, we isolated highly purified promyelocyte populations from normal and young preleukemic animals, carried out...
Bronchopneumonia was observed at the necropsy of a North Atlantic pilot whale (Globicephala melaena). The areas necrosis were well circumscribed clusters, disseminated throughout left lung. Streptococcus equi isolated in pure culture from lung parenchyma, pharynx and pericardial fluid.
Abstract Review: ca. 100 refs.
Introduction Acute lung injury (ALI) is an inflammatory disease, which manifests itself in patients as acute respiratory distress syndrome (ARDS). Our lab previously showed that hypoxia inducible transcription factor (HIF) 1A up‐regulates glycolytic capacity AT II cells during ALI a compensatory mechanism to dampen inflammation with the unknown. In tumor associated macrophages, lactate, end product of glycolysis can shift macrophages anti‐inflammatory Arg‐1 + phenotype. We hypothesized...