A5032957673- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Nail Diseases and Treatments
- Phytoplasmas and Hemiptera pathogens
- Fungal and yeast genetics research
- Antimicrobial Peptides and Activities
- Microbial Metabolic Engineering and Bioproduction
- T-cell and Retrovirus Studies
- Plant Disease Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Probiotics and Fermented Foods
- Biofuel production and bioconversion
- Legionella and Acanthamoeba research
- Parasitic Infections and Diagnostics
- Biochemical and Structural Characterization
- Proteoglycans and glycosaminoglycans research
- Streptococcal Infections and Treatments
- Entomopathogenic Microorganisms in Pest Control
- Infectious Diseases and Mycology
- Insect Resistance and Genetics
- Bacteriophages and microbial interactions
- Food Allergy and Anaphylaxis Research
Universidade de Brasília
2013-2024
University of Wisconsin–Madison
2013
Paracoccidioidomycosis (PCM) is a systemic disease endemic to most of Latin America, with greatest impact in rural areas. The taxonomic status one the best studied Paracoccidioides isolates (Pb01) as P. brasiliensis remains unresolved due its genomic differences from other three previously described phylogenetic species (S1, PS2 and PS3; Carrero et al., 2008. Fungal Genet. Biol. 45, 605). Using genealogic concordance method recognition (GCPSR) via maximum parsimony Bayesian analysis, we...
The fungal genus Sporothrix includes at least four human pathogenic species. One of these species, S. brasiliensis, is the causal agent a major ongoing zoonotic outbreak sporotrichosis in Brazil. Elsewhere, sapronoses are caused by schenckii and globosa. aims on this comparative genomic study are: 1) to explore presence virulence factors brasiliensis; 2) compare which cat-transmitted infects both humans cats with schenckii, mainly pathogen; 3) two species other pathogens (Onygenales) similar...
Quorum sensing (QS) is a cell density-dependent mechanism of communication between microorganisms, characterized by the release signaling molecules that affect microbial metabolism and gene expression in synchronized way. In this study, we investigated behaviors mediated conditioned medium (CM) pathogenic encapsulated fungus Cryptococcus neoformans. CM produced dose-dependent increases growth planktonic biofilm cells, glucuronoxylomannan release, melanin synthesis, important virulence...
Virulence of Cryptococcus neoformans for mammals, and in particular its intracellular style, was proposed to emerge from evolutionary pressures on natural environment by protozoan predation, which promoted the selection strategies that allow survival macrophages. In fact, Acanthamoeba castellanii ingests yeast cells, then can replicate intracellularly. addition, most fungal factors needed establish infection mammalian host are also important within amoeba. To better understand origin C....
Cryptococcus spp. are human pathogens that cause 181,000 deaths per year. In this work, we systematically investigated the virulence attributes of clinical isolates and correlated them with patient data to better understand cryptococcosis. We collected 66 C. neoformans 19 gattii analyzed multiple phenotypes host-pathogen interaction outcomes. tended melanize faster more intensely produce thinner capsules in comparison gattii. also observed correlations match previous studies, such as between...
Caves serve as natural reservoirs for diverse microbial species due to their unique biotic and abiotic conditions. Histoplasma spp. is frequently associated with guano-enriched soil, low luminosity, high humidity, particularly in Latin America, a region highly endemic histoplasmosis. Despite the continent's biomes, local environmental host distributions of remain poorly understood. To address this knowledge gap, we conducted -specific quantitative PCR (qPCR) assay targeting hc100 gene on...
Paracoccidioides spp. are thermodimorphic fungi that cause a neglected tropical disease (paracoccidioidomycosis) is endemic to Latin America. These inhabit the soil, where they live as saprophytes with no need for mammalian host complete their life cycle. Despite this, developed sophisticated virulence attributes allowing them not only survive in tissues but also disease. A hypothesis selective pressures driving emergence or maintenance of soil interaction predators such amoebae and...
Sporotrichosis is a subcutaneous infection caused by fungi from the genus Sporothrix. It transmitted inoculation of infective particles found in plant-contaminated material or diseased animals, characterizing classic sapronotic and emerging zoonotic transmission, respectively. Since 1998, southeastern Brazil has experienced sporotrichosis epidemic S. brasiliensis, centred state Rio de Janeiro. Our observation feline cases Brasília (Midwestern Brazil), around 900 km away Janeiro, led us to...
Lactic acid (LA) production has seen significant progress over the past ten years. LA increased economic importance due to its broadening use in different sectors such as food, medicine, polymer, cosmetic, and pharmaceutical industries. bioprocesses using microorganisms are economically viable compared chemical synthesis can benefit from metabolic engineering for improved productivity, purity, yield. Strategies optimize productivity on strain improvement end include modifying routes, adding...
is one of the agents invasive candidiasis, a life-threatening disease strongly associated with hospitalization, particularly among patients in intensive care units central venous catheters. This study aimed to evaluate synergistic activity antifungal peptide ToAP2 combined fluconazole against
ABSTRACT Levels of the anaphylatoxin C3a are increased in patients with asthma compared those nonasthmatics and increase further still during exacerbations. However, role sensitization to allergen is poorly understood. Sensitization fungal allergens, such as Aspergillus fumigatus , a strong risk factor for development asthma. Exposure chitin, structural polysaccharide cell wall, induces innate allergic inflammation may promote allergens. Here, we found that coincubation chitin serum or...
Lactic acid is the monomeric unit of polylactide (PLA), a bioplastic widely used in packaging, automotive, food, and pharmaceutical industries. Previously, yeast Komagataella phaffii was genetically modified for production lactate from glycerol. For this, bovine L-lactate dehydrogenase- (LDH)-encoding gene inserted encoding pyruvate decarboxylase (PDC) disrupted, resulting GLp strain. This showed yield 67% L-lactic 20% arabitol as by-product batches with oxygen limitation. Following up on...
In order to survive and cause disease, microbial pathogens must be able proliferate at the temperature of their infected host. We identified novel features associated with thermotolerance in opportunistic fungal pathogen Cryptococcus neoformans using a random insertional mutagenesis strategy, screening for mutants defective growth 37°C. Among several thermosensitive mutants, we one bearing disruption gene predicted encode Ape4 aspartyl aminopeptidase protein. metalloproteases other fungi,...
Abstract Background Mycelium-to-yeast transition in the human host is essential for pathogenicity by fungus Paracoccidioides brasiliensis and both cell types are therefore critical to establishment of paracoccidioidomycosis (PCM), a systemic mycosis endemic Latin America. The infected population about 10 million individuals, 2% whom will eventually develop disease. Previously, transcriptome analysis mycelium yeast cells resulted assembly 6,022 sequence groups. Gene expression analysis, using...
Paracoccidioidomycosis (PCM) is a systemic mycosis endemic to Latin America. Etiological agents are Paracoccidioides species that diverge phylogenetically throughout South America.This study aimed document the epidemiology of PCM in Venezuela.We have performed retrospective cross-sectional descriptive 31,081 clinical records patients from two reference centres during 65 years (1954-2019).PCM diagnosis was confirmed 745 patients. Chronic most prevalent form (90.06% cases); 80.67% were male...
Paracoccidioides brasiliensis is a thermo-dimorphic fungus that causes human systemic mycosis with high incidence in Latin America. Owing to their participation the control of pathogen morphogenesis, differentiation and virulence, it was decided characterize ras genes P. brasiliensis. ras1 ras2 were identified be coding for two different proteins identity. The transcriptional pattern investigated by reverse transcription PCR (RT-PCR) during mycelium-to-yeast (M→Y) transition, heat shock at...
Hyaluronic acid (HA) is a biopolymer formed by UDP-glucuronic and UDP-N-acetyl-glucosamine disaccharide units linked β-1,4 β-1,3 glycosidic bonds. It widely employed in medical cosmetic procedures. HA synthesized hyaluronan synthase (HAS), which catalyzes the precursors’ ligation cytosol, elongates polymer chain, exports it to extracellular space. Here, we engineer Ogataea (Hansenula) polymorpha for production inserting genes encoding UDP-glucose 6-dehydrogenase, production, HAS. Two...
Gti1/Pac2 transcription factors occur exclusively in fungi and their roles vary accord-ing to species, including regulating morphological transition virulence, mating secondary metabolism. Many of these functions are important for fungal pathogenesis. We therefore hypothesized that one the two proteins this family Cryptococcus neoformans, a major pathogen humans, would also control virulence-associated cel-lular processes. Elimination protein C. neoformans results reduced poly-saccharide...
Cytosolic pyruvate is an essential metabolite in lactic acid production during microbial fermentation. However, under aerobiosis, transported to the mitochondrial matrix by carrier (MPC) and oxidized cell respiration. Previous reports using Saccharomyces cerevisiae or Aspergillus oryzae have shown that of pyruvate-derived chemicals improved deleting MPC1 gene. A previous lactate-producing K. phaffii strain engineered our group was used as a host for deletion In addition, expression bacterial...