A5047668057- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Advanced biosensing and bioanalysis techniques
- Parasitic Infections and Diagnostics
- Streptococcal Infections and Treatments
- SARS-CoV-2 detection and testing
- Nail Diseases and Treatments
- Advanced Proteomics Techniques and Applications
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Antimicrobial Resistance in Staphylococcus
- Plant Pathogens and Fungal Diseases
- COVID-19 Clinical Research Studies
- Peptidase Inhibition and Analysis
- Poxvirus research and outbreaks
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
- Orthopedic Infections and Treatments
- Animal Virus Infections Studies
- Infectious Diseases and Mycology
- Actinomycetales infections and treatment
University of Wisconsin–Madison
2013-2022
UW Health University Hospital
2017-2020
Oklahoma State Department of Health
2018
Engineering Associates (United States)
2017
SomaLogic (United States)
2010
Background The interrogation of proteomes ("proteomics") in a highly multiplexed and efficient manner remains coveted challenging goal biology medicine. Methodology/Principal Findings We present new aptamer-based proteomic technology for biomarker discovery capable simultaneously measuring thousands proteins from small sample volumes (15 µL serum or plasma). Our current assay measures 813 with low limits detection (1 pM median), 7 logs overall dynamic range (∼100 fM–1 µM), 5% median...
Abstract Interrogation of the human proteome in a highly multiplexed and efficient manner remains coveted challenging goal biology. We present new aptamer-based proteomic technology for biomarker discovery capable simultaneously measuring thousands proteins from small sample volumes (15 [mu]L serum or plasma). Our current assay allows us to measure ~800 with very low limits detection (1 pM average), 7 logs overall dynamic range, 5% average coefficient variation. This is enabled by generation...
As part of public health preparedness for infectious disease threats, CDC collaborates with other U.S. officials to ensure that the Laboratory Response Network (LRN) has diagnostic tools detect Orthopoxviruses, genus includes Variola virus, causative agent smallpox. LRN is a network state and local health, federal, Department Defense (DOD), veterinary, food, environmental testing laboratories. developed, Food Drug Administration (FDA) granted 510(k) clearance* Non-variola Orthopoxvirus...
Repeating the BinaxNOW antigen test for severe acute respiratory syndrome coronavirus 2 using groups of readers within 30 minutes resulted in high concordance (98.9%) 2110 encounters. Same-day repeat testing did not significantly improve sensitivity (77.2% to 81.4%) while specificity remained (99.6%).
Group A streptococcus (GAS) is responsible for 15%-30% of cases acute pharyngitis in children. Macrolides such as azithromycin have become popular treating GAS pharyngitis. We report macrolide resistance rates a primary care setting our geographic area over the past 5 years and discuss implications making treatment decisions. Throat swabs were collected from children with May 2011 to 2015 Madison, Wisconsin. Susceptibility testing was performed erythromycin clindamycin using Kirby-Bauer disk...
Blastomyces is a dimorphic fungus that infects persons with or without underlying immunocompromise. To date, no study has compared the clinical features and outcomes of blastomycosis between immunocompromised immunocompetent persons.A retrospective adult patients proven from 2004-2016 was conducted at University Wisconsin. Epidemiology, features, were analyzed among solid-organ transplantation (SOT) recipients, non-SOT immunocompromise (non-SOT IC), (NIC).A total 106 cases met inclusion...
Blastomyces dermatitidis, a dimorphic fungus and the causative agent of blastomycosis, is widely considered an extracellular pathogen, with little evidence for facultative intracellular lifestyle. We infected mice spores, that is, infectious particle, via pulmonary route studied residence, transition to pathogenic yeast, replication inside lung cells. Nearly 80% spores were cells at 24 h postinfection 10(4) spores. Most located alveolar macrophages, smaller numbers in neutrophils dendritic...
ABSTRACT Levels of the anaphylatoxin C3a are increased in patients with asthma compared those nonasthmatics and increase further still during exacerbations. However, role sensitization to allergen is poorly understood. Sensitization fungal allergens, such as Aspergillus fumigatus , a strong risk factor for development asthma. Exposure chitin, structural polysaccharide cell wall, induces innate allergic inflammation may promote allergens. Here, we found that coincubation chitin serum or...
Blastomyces spp, the etiologic agents of blastomycosis, are endemic dimorphic fungi that require prolonged antifungal therapy, which can be complicated by adverse drug effects. Isavuconazonium sulphate (ISA) is a triazole with in vitro and vivo activity against but there paucity clinical data supporting its use for treatment blastomycosis.This retrospective case series identified 14 patients blastomycosis at least partially treated ISA University Wisconsin between 2015 2019. Treatment...
OBJECTIVE To describe the investigation and control of a cluster Serratia marcescens bacteremia in 505-bed tertiary-care center. METHODS Cluster cases were defined as all patients with S. between March 2 April 7, 2014, who found to have identical or related blood isolates determined by molecular typing pulsed-field gel electrophoresis. Cases compared using bivariate analysis controls admitted at same time service cases, 4:1 ratio. RESULTS In total, 6 developed within 48 hours after admission...
Abstract Repeating the BinaxNOW antigen test for SARS-CoV-2 by two groups of readers within 30 minutes resulted in high concordance (98.9%) 2,110 encounters. sensitivity was 77.2% (258/334) compared to real-time reverse transcription-polymerase chain reaction. testing on same day did not significantly improve while specificity remained high.
Blastomycosis is endemic in Wisconsin with Blastomyces dermatitidis and B. gilchristii responsible for infections. Urine antigen testing a non-invasive diagnostic method blastomycosis up to 93% test sensitivity. However, the test's sensitivity has not been evaluated relationship infections.We aimed assess physician use of urine assay its infections retrospective study. Culture confirmed clinical cases from 2008-2016 were identified within Marshfield Clinic Health System (MCHS) UW Hospital...
In the summer of 2015, many individuals visiting Little Wolf River in Waupaca County were exposed to pathogenic fungus, Blastomyces. Over time, 59 confirmed and 39 probable cases reported Wisconsin Department Health Services (W-DHS), making this one largest outbreaks recent state history. Though most instances blastomycosis are not associated with common source outbreaks, such as highlight need for vigilance regarding preventable cause death. Wisconsin, an average 118.6 (range, 84-174)...
To improve understanding of the antibody response to SARS-CoV-2 infection, we examined seroprevalence, incidence and seroconversion among a cohort young adults living on university campuses during fall 2020.At beginning (semester start) end end) an 11-week period, serum collected from 107 students was tested using qualitative Abbott Architect IgG AdviseDx II assays. Results were matched interim weekly surveillance viral testing symptom data.With assay, 15 (14.0%) seropositive at semester...
University students commonly received COVID-19 vaccinations before returning to U.S. campuses in the Fall of 2021. Given likely immunologic variation among based on differences type primary series and/or booster dose vaccine received, we conducted serologic investigations September and December 2021 a large university campus Wisconsin assess anti-SARS-CoV-2 antibody levels.We collected blood samples, demographic information, illness vaccination history from convenience sample students. Sera...
The Wisconsin Clinical Laboratory Network (WCLN) at the University of Wisconsin-Madison is a partnership 138 clinical and public health laboratories (as February 2019) coordinated by State Hygiene. This article describes WCLN, its current activities, lessons learned through this partnership. A laboratory technical advisory group, which consists representatives from laboratories, provides perspective to WCLN fosters communication among laboratories. Activities resources available include...
Blastomyces dermatitidis is a dimorphic fungus endemic to the United States and Canada. Although both Histoplasma are found in similar geographic regions, many times more likely cause dissemination immunocompetent host, frequently involving bone. However, given indolent nature of this fungal infection prevalent bacterial etiologies osteomyelitis, diagnosis treatment often significantly delayed. We review 2 pediatric cases that initially presented with isolated orthopedic symptoms without...
BACKGROUND:Actinomucor elegans is an unusual cause of mucormycosis and can be difficult to identify by conventional methods. Mucormycosis has a very high mortality rate, especially among immunocompromised individuals. Due the morbid progressive nature opportunistic fungal infections, early diagnosis paramount for effective disease management. Matrix-assisted laser desorption/ionization time flight mass spectrometry (MALDI) Sanger sequencing are useful methods rapid pathogens. CASE REPORT:We...