A5057038894- Optical Coherence Tomography Applications
- Single-cell and spatial transcriptomics
- Bacterial Infections and Vaccines
- Advanced Fluorescence Microscopy Techniques
- Cerebrospinal fluid and hydrocephalus
- Innovative Microfluidic and Catalytic Techniques Innovation
- Immune cells in cancer
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Cell Image Analysis Techniques
- Photonic and Optical Devices
- Laser Applications in Dentistry and Medicine
- Retinal Diseases and Treatments
- Cancer Genomics and Diagnostics
- Electrowetting and Microfluidic Technologies
- Nanomaterials and Printing Technologies
- Mesoporous Materials and Catalysis
- Microfluidic and Capillary Electrophoresis Applications
- Retinal Imaging and Analysis
- Multiple Sclerosis Research Studies
- Semiconductor Lasers and Optical Devices
- Fluid Dynamics and Thin Films
University of Rochester Medical Center
2022
Massachusetts General Hospital
2019-2022
Weatherford College
2022
Harvard University
2019-2022
Center for Systems Biology
2019-2022
Jožef Stefan Institute
2019-2021
Carl Zeiss (United States)
2020
European Molecular Biology Laboratory
2018-2019
European Molecular Biology Laboratory
2018-2019
In this work, we quantify the mechanical properties of extra-cellular matrix (ECM) in live zebrafish using Brillouin microscopy. Optimization imaging conditions and parameters, combined with careful spectral analysis, allows us to resolve thin ECM distinguish its frequency shift, a proxy for properties, from surrounding tissue. High-resolution mapping further enables direct measurement thickness label-free in-vivo. We find be ~500 nm thick, very good agreement electron microscopy...
Tissue function depends on cellular organization. While the properties of individual cells are increasingly being deciphered using powerful single-cell sequencing technologies, understanding their spatial organization and temporal evolution remains a major challenge. Here, we present Image-seq, technology that provides transcriptional data isolated from specific locations under image guidance, thus preserving information target cells. It is compatible with in situ vivo imaging can document...
Optical coherence tomography angiography (OCTA) imaging is a valuable tool for the visualization of retinal vasculature at an unprecedented level details. However, due to relatively long time-interval between repeated scans in conventional OCTA scanning protocol, flow signal suffers from low dynamic range and loss velocity-intensity correlation. The ability distinguish fast slow retina may provide powerful assessment early-stage diseases such as vein occlusion. Here, we report method detect...
Droplets with predefined sizes have been controllably produced at the tip of a micro-capillary immersed in an external fluid while tracking high Q-factor whispering gallery modes (WGM). The were fitted to model give precise real-time size measurement, which was used as feedback control pressure capillary and release droplet from when it reached target size. In this way dispersion highly monodisperse droplets anywhere range 5 μm 50 produced. To fabricate solid beads, made liquid photopolymer...
Abstract Interactions between the immune and central nervous systems strongly influence brain health. Although blood-brain barrier restricts this crosstalk, we now know that meningeal gateways through border tissues, particularly dural lymphatic vessels allow cerebrospinal fluid outflow, facilitate intersystem communication. Here observe exits into skull bone marrow. Fluorescent tracers injected cisterna magna of mice travel hundreds sub-millimeter channels calvarial During meningitis,...
In this work, we quantify the mechanical properties of extra-cellular matrix (ECM) in live zebrafish using Brillouin microscopy. Optimization imaging conditions and parameters, combined with careful spectral analysis, allows us to resolve thin ECM distinguish its frequency shift from surrounding tissue. High-resolution mapping further enables directly measure thickness label-free in-vivo. We find be ~400nm thick, excellent agreement electron microscopy quantification. Our results open door...
Bio-integrated lasers, that are lasers implanted into cells and tissues, gaining interest from the research community. Here we show how microlasers microcavities based on whispering gallery modes can be used for sensing different processes in biological materials including inside cells. By making of a predefined size they also to encode some information cell tracking. Sensing tracking applied highly scattering tissues.
Abstract Tissue function depends on proper cellular organization. While the properties of individual cells are increasingly being deciphered using powerful single-cell sequencing technologies, understanding their spatial organization and temporal evolution remains a major challenge. Here, we present Image-seq, technology that provides transcriptional data isolated from specific locations under image guidance, thus preserving information target cells. It is compatible with in situ vivo...