A5087994868- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Mycobacterium research and diagnosis
- Organ Transplantation Techniques and Outcomes
- Organ Donation and Transplantation
- Microscopic Colitis
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Renal Transplantation Outcomes and Treatments
- Immune Response and Inflammation
- Hematopoietic Stem Cell Transplantation
- Gastrointestinal motility and disorders
- Pediatric health and respiratory diseases
- Clinical Nutrition and Gastroenterology
- IL-33, ST2, and ILC Pathways
- Dermatology and Skin Diseases
- LGBTQ Health, Identity, and Policy
- Diagnosis and treatment of tuberculosis
- Liver Disease and Transplantation
- Helicobacter pylori-related gastroenterology studies
- Inflammatory Bowel Disease
- Cytomegalovirus and herpesvirus research
- Innovations in Medical Education
- Biomedical Ethics and Regulation
- Whipple's Disease and Interleukins
University of Maryland, Baltimore
2020-2025
University of Maryland Medical Center
2022-2023
Rutgers New Jersey Medical School
2019
Rutgers, The State University of New Jersey
2019
New York University
2015-2018
Icahn School of Medicine at Mount Sinai
2015
University of Chicago
2009
University of Chicago Medical Center
2008
Abstract Current immunosuppressants effectively suppress adaptive and innate immune responses, but their broad, antigen-non-specific effects often result in significant complications. Here, we conducted a systematic investigation of drug on gut microbiome, metabolic pathways, lymphoid architecture lymphocyte trafficking four major classes including tacrolimus, prednisone, mycophenolate mofetil (MMF), Fingolimod (FTY). We show that induced progressive alterations the microbiome where initial...
Abstract The beneficial effects attributed to Bifidobacterium are largely their immunomodulatory capabilities, which likely be species- and even strain-specific. However, strain-specificity in direct indirect immune modulation remain uncharacterized. We have shown that B. pseudolongum UMB-MBP-01, a murine isolate strain, is capable of suppressing inflammation reducing fibrosis vivo. To ascertain the mechanism driving this activity determine if it specific we compared porcine tropic strain...
Lymph node (LN) fibroblastic reticular cells (FRCs) define LN niches and regulate lymphocyte homeostasis through producing diverse extracellular matrix (ECM) components. We examined the role of ECM laminin α4 (Lama4) using FRC-Lama4 conditional KO Pdgfrb-Cre-/- × Lama4fl/fl mice. Single-cell RNA-sequencing (scRNA-Seq) data showed promoter gene Pdgfrb was exclusively expressed in FRCs. Depleting reduced Tregs dendritic cells, decreased high endothelial venules, impaired conduit system,...
Abstract Intrinsic metabolism shapes the immune environment associated with suppression and tolerance in settings such as organ transplantation cancer. However, little is known about metabolic activities an immunosuppressive environment. In this study, we employed metagenomic, metabolomic, immunological approaches to profile early effects of immunosuppressant drug tacrolimus, antibiotics, or both gut lumen circulation using a murine model. Tacrolimus induced rapid profound alterations within...
Background. Despite ongoing improvements to regimens preventing allograft rejection, most cardiac and other organ grafts eventually succumb chronic vasculopathy, interstitial fibrosis, or endothelial changes, graft failure. The events leading rejection are still poorly understood the gut microbiota is a known driving force in immune dysfunction. We previously showed that dysbiosis profoundly influences outcome of vascularized allografts subsequently identified biomarker species associated...
Positron emission tomography (PET) using 18-fluorodeoxyglucose (18-FDG) is a noninvasive, functional imaging modality most often used to assess cancer. The aim of this study was perform PET/computed (CT) on patients with quiescent ulcerative colitis (UC) understand the limits technology for assessing inflammatory activity.We identified diagnosed UC in strictly defined remission state. PET/CT performed standard fashion, approximately 10 mCi 18-FDG 60-minute uptake delay. Uptake each 4 colonic...
OPEN ACCESSJuly 21, 2020The Case of Sean Smith: A Three-Part Interactive Module on Transgender Health for Second-Year Medical Students Marc G. Berenson, MD, Samuel J. Gavzy, MS, Lissette Cespedes, Aayush Gabrani, Michelle Davis, Kai Ingram, Dean Gailey, John Paul Sánchez, MPH MD https://orcid.org/0000-0002-0731-5326 Resident, Department Emergency Medicine, Rutgers New Jersey School , MS General Surgery, University Maryland Medicine Assistant Professor Pediatrics, Ingram Senior Environmental...
The Institute of Medicine's 2011 report on lesbian, gay, bisexual, and transgender (LGBT) health the legalization same-sex marriage are just two numerous milestones that have hastened medical schools' efforts to prepare trainees address needs LGBT community members. Early awareness sexual diversity through self- peer introspection video-based education can help build a foundation towards providing affirming care patients.The Kern model was used develop, implement, evaluate an interactive...
Transplant recipients require lifelong, multimodal immunosuppression to prevent rejection by reducing alloreactive immunity. Rapamycin, a mechanistic target of rapamycin (mTOR) inhibitor, is known modulate adaptive and innate immunity, while the full spectrum its immunosuppressive mechanisms remains incompletely understood. Given broad expression mTOR, we investigated understudied effects on lymph node (LN) architecture, leukocyte trafficking, gut microbiome metabolism after 3, 7, 30 days...
Fibroblastic reticular cells (FRCs) play important roles in tolerance by producing laminin α4 (Lama4) and altering lymph node (LN) structure function. The present study revealed the specific of extracellular matrix Lama4 regulating LN conduits using FRC-specific KO mouse strains. FRC-derived maintained conduit fiber integrity, as its depletion altered morphology reduced homeostatic flow. regulated lymphotoxin β receptor (LTβR) pathway, which is critical for integrity. Depleting LTβR FRCs...
Intrinsic metabolism shapes the immune environment associated with suppression and tolerance in settings such as organ transplantation cancer. However, little is known about metabolic activities an immunosuppressive environment. In this study, we employed metagenomic, metabolomic, immunological approaches to profile early effects of immunosuppressant drug tacrolimus, antibiotics, or both gut lumen circulation using a murine model. Tacrolimus induced rapid profound alterations within two days...
Vitamin D (VD) has immunomodulatory properties, but whether immune cell expression of the VD receptor (VDR) impacts costimulatory blockade induced cardiac allograft survival is not known.To localize effects VDR deficiency to hematopoietic cells and avoid metabolic consequences systemic deficiency, we produced bone marrow (BM)-chimeric mice by transplanting lethally irradiated C57BL/6 with congenic or wild type BM. After reconstitution, characterized baseline profiles transplanted chimeras...
Abstract The beneficial effects attributed to Bifidobacterium are largely their immunomodulatory capabilities, which likely be species- and even strain-specific. However, strain-specificity in direct indirect immune modulation remain uncharacterized. We have shown that B. pseudolongum UMB-MBP-01, a murine isolate, is capable of suppressing inflammation reducing fibrosis vivo . To ascertain the mechanism driving this activity determine if it specific we compared type strain ATCC25526 porcine...
Increasingly complex and long-range donor organ allocation routes coupled with implementation of unmanned aerial vehicles (UAVs) have prompted investigations the conditions affecting organs once packaged for shipment. Our group has previously demonstrated that different modes transport exert unique environmental stressors, in particular vibration. Using a mouse heart transplant model, we vibrational forces tangible, cellular effects form cardiomyocyte apoptosis cytoskeletal derangement....