A5057514389- Immune Cell Function and Interaction
- HIV Research and Treatment
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- HIV-related health complications and treatments
- Immune Response and Inflammation
- HIV/AIDS Research and Interventions
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Diabetes and associated disorders
- Antifungal resistance and susceptibility
- HIV/AIDS drug development and treatment
- Reproductive System and Pregnancy
- Pneumocystis jirovecii pneumonia detection and treatment
- vaccines and immunoinformatics approaches
- Health disparities and outcomes
- E-Learning and Knowledge Management
- CAR-T cell therapy research
- Public Health and Social Inequalities
- Blood disorders and treatments
- Health and Lifestyle Studies
- Fungal Infections and Studies
- Influenza Virus Research Studies
- Digestive system and related health
- Antimicrobial Peptides and Activities
Universidad de Antioquia
2009-2024
Massey University
2021-2023
AgResearch
2021-2023
Universidad del Norte
2017
Rush University Medical Center
2005-2008
Pontifical Catholic University of Peru
1998
Ministry of Health
1997
Identification of human CD1d-restricted T-cell receptor (TCR)-invariant natural killer T (iNKT) cells has been dependent on utilizing combinations monoclonal antibodies or CD1d tetramers, which do not allow for the most specific analysis this subpopulation. A novel antibody (clone 6B11), invariant CDR3 loop canonical Valpha24Jalpha18 TCR alpha chain, was developed and used to specifically characterize iNKT cells. In healthy individuals studied up 1 year, a wide but stable frequency...
Plasmacytoid dendritic cells (pDC) contribute to antiviral immunity mainly through recognition of microbial products and viruses via intracellular Toll-like receptor 7 (TLR7) or TLR9, resulting in the production type I interferons (IFNs). Although reduce viral burden acute phase infection, their role chronic is unclear. The presence elevated plasma IFN-alpha levels advanced HIV disease its association with translocation infection lead us hypothesize that could immune activation. Blocking...
Statins have been shown to modulate the number and suppressive function of CD4 + FOXP3 T cells (Treg) in inflammatory conditions. However, it is not well established whether statin could also affect Treg absence inflammation. To address this question, eighteen normocholesterolemic male subjects were treated with lovastatin or atorvastatin daily for 45 days. The frequency phenotype circulating evaluated at days 0, 7, 30, 45. mRNA levels FOXP3, IDO, TGF- β , IL-10 measured cells. We found that...
Abstract CD1d-restricted invariant NK T (iNKT) cells and dendritic (DCs) have been shown to play crucial roles in various types of immune responses, including TLR9-dependent antiviral responses initiated by plasmacytoid DCs (pDCs). However, the mechanism which this occurs is enigmatic because TLRs are absent iNKT human pDCs do not express CD1d. To explore process, were activated with CpG oligodeoxyribonucleotides, stimulated secretion several cytokines such as type I TNF-α. These other...
Peripheral blood T-cells from untreated HIV-1-infected patients exhibit reduced immune responses, usually associated with a hyperactivated/exhausted phenotype compared to HAART treated patients. However, it is not clear whether ameliorates this altered of in the gastrointestinal-associated lymphoid tissue (GALT), main site for viral replication. Here, we peripheral and GALT two groups chronically patients: active replication, on suppressive HAART. We characterized T-cell by measuring PD-1,...
Summary Oligodeoxynucleotides (ODN) with unmethylated deoxycytidyl‐deoxyguanosine dinucleotides (CpG‐ODNs) stimulate Toll‐like receptor 9 (TLR9) in plasmacytoid dendritic cells (pDC) and B activate innate adaptive immunity. Three classes of synthetic CpG‐ODNs, class A, C, through TLR9; our goal was to evaluate their effect on from human immunodeficiency virus (HIV)‐1 + individuals. We compared the frequencies unstimulated activation status immune effector HIV‐1 – Fewer pDC, myeloid (mDC),...
Although very inefficient, sexual transmission of HIV-1 is responsible for more than 80% infections worldwide. Yet, the presence HIV in spermatozoa has been a matter debate. The aim this study was to evaluate nucleic acids and distribution mannose receptors sperm cells, determine semen parameters cytokine levels ejaculates from HIV-positive patients. non-seminal cells purified revealed by light microscopy, flow cytometry RT-PCR. were evaluated nested PCR; distributions on surface determined...
HIV infection induces immune alterations, mainly in gut mucosa, where the main target cells reside. However, evolution of is variable among infected individuals, as evidenced by controllers who exhibit low or undetectable viral load absence treatment. The aim this study was to evaluate frequency, phenotype and activity T NK peripheral blood mucosa a cohort Colombian controllers. Blood biopsies were included. frequency activation status performed flow cytometry. In addition, Gag-stimulated...
Highly active antiretroviral therapy produces a significant decrease in HIV-1 replication and allows an increase the CD4 T-cell count, leading to incidence of opportunistic infections mortality. However, cost, side effects complexity regimens have underscored immediate need for additional therapeutic approaches. Statins exert pleiotropic through variety mechanisms, among which there are several immunoregulatory effects, related unrelated their cholesterol-lowering activity that can be useful...
There are 2 new phenotypes of HIV-1-positive individuals who exhibit a spontaneous and sustained control viral replication at least for 1 year without antiretroviral therapy (elite controllers <50 copies/mL viremic <2000 copies/mL). Mechanisms related to this poorly understood.The study included HIV-1 (patients with diagnosis, highly active naive, loads less than 2000 copies/mL) progressors (viral load >2500 copies/mL, CD4 T-cell count >250 cells/μL the time sampling). The expression soluble...
Given that highly active antiretroviral therapy (HAART) has been demonstrated useful to restore immune competence in type-1 human immunodeficiency virus (HIV-1)-infected subjects, we evaluated the specific antibody response influenza vaccine a cohort of HIV-1-infected children on HAART so as analyze quality this patients under therapy. Sixteen and 10 HIV-1 seronegative controls were immunized with commercially available trivalent inactivated containing strains A/H1N1, A/H3N2, B. Serum...
Infants with biallelic IL7R loss-of-function variants have severe combined immune deficiency (SCID) characterized by the absence of autologous T lymphocytes, but normal counts circulating B and NK cells (T-B+NK+ SCID). We report 6 adults (aged 22 to 59 years) from 4 kindreds 3 ancestries (Colombian, Israeli Arab, Japanese) carrying homozygous IL7 resulting in immunodeficiency (CID). Deep immunophenotyping revealed relatively and/or proportions myeloid, B, NK, innate lymphoid cells. By...
Background: The immunological benefits of highly active antiretroviral therapy (HAART) in HIV-1-infected children include reconstitution CD4+ T-cell count and functional activity. effect HAART on innate immune cells has not been well established. Aim: To characterize responses HAART-treated children. Patients Methods: 23 stable uninfected were evaluated. frequency peripheral blood was determined by flow cytometry activity evaluated using Toll-like receptor agonists. Results: Compared with...
HIV replication control is important to reduce AIDS progression. We determined frequency and activation status of immune cells in spontaneous controllers vs. individuals with highly active antiretroviral therapy (HAART)-controlled viral load. exhibited significantly higher CD4+ T myeloid dendritic compared HAART-controlled Additionally, have a lower percentage expressing markers on CD8+ cells, natural killer cells. These findings suggest that during infection, conservation normal...
HIV infection induces several gradual alterations on the peripheral and mucosal immune systems, with different magnitudes between infected individuals. In this regard, spontaneous controllers exhibit either low or undetectable viral loads in absence of treatment along decreased compared to progressors. Yet, it is unknown how similar parameters are when comparing uninfected individuals.We evaluated a cohort 11 who were 20 seronegative donors. Peripheral blood (PB) gut associated lymphoid...