Gene induction and categorical reprogramming during in vitro human endometrial fibroblast decidualization. Physiol Genomics 7: 135–148, 2001. First published September 21, 2001; 10.1152/physiolgenomics.00061.2001.—Human decidual fibroblasts undergo a differentiative commitment to the acquisition of endocrine, metabolic, structural cell functions process known as Decidualization is critical for embryo implantation placental function. We characterized gene expression pattern kinetics...

Endometrial stromal differentiation (decidualization) is essential for implantation of the developing blastocyst. Because prostaglandins (PGs) are synthesized in endometrium, and PG-binding sites have been demonstrated proliferative endometrial cell (the precursor decidual cell), experiments were performed to determine whether PGs involved process decidualization. Human cells cultured 18 days Dulbeco's Modified Eagle's Medium-2% fetal bovine serum with 1 microM medroxyprogesterone acetate...

Term decidual tissue consists of terminally differentiated cells, bone marrow-derived and fibroblast cells. Since undifferentiated cells are fibroblast-like the endometrial stroma, possibility exists that in term decidua To test this hypothesis, a purified population was isolated from treated under conditions known to induce differentiation stromal By flow cytometry immunocytochemistry, were shown be free expressing cell-surface antigens epithelial cell marker cytokeratin. In addition, they...

Interleukin-1 beta (IL-1 beta), which modulates cell proliferation and differentiation in a number of types, is present human endometrial stromal cells. However, both the function IL-1 endometrium factors that modulate its expression cells are unknown. To examine effects on decidualization, proliferative were cultured for 12 days medium (Dulbecco's Modified Eagle's with 2% fetal bovine serum) containing 1 microM medroxyprogesterone acetate, 10 nM estradiol, prostaglandin E2 (PGE2) without...

Background Coxsackievirus B (CVB) is the most common cause of viral myocarditis. It targets cardiomyocytes through coxsackie and adenovirus receptor, which highly expressed in fetal heart. We hypothesized CVB3 can precipitate congenital heart defects when infection occurs during critical window gestation. Methods Results infected C57Bl/6 pregnant mice with time points early gestation (embryonic day [E] 5, E7, E9, E11). used different titers to examine possible dose‐response relationship...

Abstract We have previously isolated insulin‐like growth factor binding protein‐4 (IGFBP‐4) from media conditioned by a neuronal cell line and detected IGFBP‐4 mRNA in selected regions of the developing adult rat brain Northern blot analysis. In this study, ontogeny regional distribution expression were determined situ hybridization histochemistry. While at embryonic day 15 was restricted to choroid plexus primordium meninges, 20 also localized basal ganglia. postnatal rat, days 1 5, present...

All-trans retinoic acid (RA) has potent effects on cell differentiation and gene expression. Previous studies have demonstrated that human endometrial stromal cells express mRNA for receptors (RARs) cellular RA-binding protein-II (CRABP-II). We examined whether RA regulates (decidualization), a critical process in preparation of the uterus blastocyst implantation. Decidualization was induced by incubating cultured with medroxyprogesterone acetate (MPA) oestradiol. defined induction...

The tissue-specific expression of a fusion gene encoding the mouse metallothionein-1 promotor and coding region human GH-releasing hormone (hGRH) was studied in transgenic mice by immunohistochemistry using an anti-hGRH serum that does not recognize endogenous GRH. hGRH immunoreactivity (GRH-IR) detected specific cells pituitary, pancreas, kidney, duodenum, lung, testis, ovary, adrenal, heart, brain. In double immunofluorescent staining, GRH-IR found some, but all, somatotrophs,...

Introduction Nucleic acid from viruses is common in peripheral blood, even asymptomatic individuals. How physiologic changes of pregnancy impact host-virus dynamics for acute, chronic, and latent viral infections not well described. Previously we found higher diversity the vagina during associated with preterm birth (PTB) Black race. We hypothesized that copy numbers plasma would show similar trends. Methods To test this hypothesis, evaluated longitudinally collected samples 23 pregnant...

We have investigated the effect of hyperprolactinaemia on secretion LH-releasing hormone (LHRH), LH and FSH in male rats PVG strain which were left intact, castrated or then implanted with either a 10 30 mm silicone elastomer capsule containing testosterone (T10 T30 respectively). Hyperprolactinaemia was produced by pituitary grafts under kidney capsule. Pituitary stalk blood, for LHRH estimation, peripheral LH, prolactin, collected alphaxalone anaesthesia. blood during three consecutive...

Abstract The expression of laminin, a major constituent endometrial cell basement membranes, is increased during differentiation human stromal cells (decidualization). To determine whether laminin plays role in decidualization, we studied the effects substrate on synthesis and release prolactin (PRL) insulin‐like growth factor binding protein‐1 (IGFBP‐1), two secretory proteins decidualized cells. Endometrial were plated as well several other extracellular matrix (ECM) (types 1 IV collagen...

Structural congenital heart disease (CHD) has not previously been linked to autoimmunity. In our study, we developed an autoimmune model of structural CHD that resembles hypoplastic left syndrome (HLHS), a life-threatening primarily affecting the ventricle. Because cardiac myosin (CM) is dominant autoantigen in disease, hypothesized immunization with CM might lead transplacental passage maternal autoantibodies and prenatal HLHS phenotype exposed fetuses. Elevated anti-CM fetal sera, as well...

The histologic distribution of a prostatic secretory protein 94 amino acids (PSP ) and its mRNA were examined simultaneously by conventional immunohistochemistry in situ hybridization histochemistry. results show localization immunoreactive PSP the epithelial cells prostate gland, thus providing strong evidence synthesis these cells. Preliminary analysis radioimmunoassay Northern blot indicates that biosynthesis pathologic tissues is variable. value as marker gland activity discussed.

ABSTRACT Vasoactive intestinal peptide (VIP) was measured by radioimmunoassay in hypophysial portal and peripheral blood from adult male female Wistar rats. The results confirmed that the concentration of VIP significantly greater than showed release into not affected removal gut, largest source VIP. Electrical stimulation median eminence, several hypothalamic nuclei, amygdala or hippocampus had no significant effect on blood, possibly because under conditions experiment spontaneous is...

Deletion analysis of the human PRL promoter in endometrial stromal cells decidualized vitro revealed a 536-bp enhancer located between nucleotide (nt) −2,040 to −1,505 5′-flanking region. The fragment ligated into thymidine kinase (TK) promoter-luciferase reporter plasmid conferred activity decidual-type but not nondecidual cells. DNase I footprint three protected regions, FP1–FP3. Transfection overlapping 100-bp fragments indicated that FP1 and FP3 each activity. Gel shift assays both bind...

A somatomammotroph cell line derived from male rat anterior pituitary (rPCO) has been established without the use of transforming agents and maintained in culture for more than 1 yr (45 passages) using Minimum Essential Medium supplemented with 10% horse serum, 5 nM T3, 2 corticosterone, 0.1 GH-releasing hormone (GRH). Peroxidase immunofluorescent staining revealed immunoreactive GH 99% rPCO cells PRL 72% cells. Within individual cells, appear to be colocalized. The storage capacity...

Transcriptional regulation of the prolactin gene in decidua differs from that pituitary. Several lines evidence strongly suggest this difference is due to and other extra-pituitary tissues by tissue-specific transcription factors, which activate distinct promoters induce expression sites compared with The human a major site gene. Here we present factor Ets-1 critical for basal decidua-type (or decidual) promoter. Overexpression significantly induces decidual promoter activity BeWo JAR cells...

The aim of this study was to examine whether the transcription factor ETS1 plays a critical role in regulation human decidualization. Decidual fibroblast cells were decidualized vitro by treatment with medroxyprogesterone, estradiol (E(2)) and dibutyryl cyclic AMP or prostaglandin E(2) absence presence an antisense oligonucleotide (oligo) that blocks translation mRNA. Control experiments performed using control oligo did not affect expression induction specific marker genes for markedly...