A5032641748- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Dialysis and Renal Disease Management
- Clostridium difficile and Clostridium perfringens research
- Central Venous Catheters and Hemodialysis
- Vascular Procedures and Complications
- Mass Spectrometry Techniques and Applications
- Renal and related cancers
- Pregnancy and preeclampsia studies
- Systemic Lupus Erythematosus Research
- Liver Disease and Transplantation
- Nosocomial Infections in ICU
- Retinoids in leukemia and cellular processes
- Birth, Development, and Health
- Pulmonary Hypertension Research and Treatments
- Renin-Angiotensin System Studies
- Microscopic Colitis
- Urticaria and Related Conditions
- Venous Thromboembolism Diagnosis and Management
- Vascular anomalies and interventions
- Peripheral Artery Disease Management
- Analytical Chemistry and Chromatography
- Vascular Malformations and Hemangiomas
- Advanced Proteomics Techniques and Applications
- Gastrointestinal motility and disorders
Wroclaw Medical University
1994-2024
Children's Specialized Hospital
2020
Łukasiewicz Research Network – PORT Polish Center for Technology Development
2017-2019
University of Regensburg
2019
Wroclaw Research Centre EIT+ (Poland)
2012-2015
Wojewódzki Szpital Specjalistyczny we Wrocławiu
2013
RWTH Aachen University
2007-2012
Sepragen Corporation (United States)
2007
Slovak Medical University
2007
Salford Royal Hospital
2007
Glomerulonephritis (GN) is a major cause of renal failure. This study sought to determine whether intrarenal injection rat mesenchymal stem cells (MSC) can preserve function in progressive model GN. Early GN (day 10), fluorescently labeled MSC localized more than 70% glomeruli, ameliorated acute failure, and reduced glomerular adhesions. Fifty days later, proteinuria had progressed controls 40 +/- 25 mg/d but stayed low MSC-treated rats (13 4 mg/d; P < 0.01). Renal on day 60 the group was...
Renal fibrosis is the final common pathway of most progressive renal diseases. C5 was recently identified as a risk factor for liver fibrosis. This study investigated role in development tubulointerstitial by (1) induction wild-type and C5(-/-) mice unilateral ureteral ligation (UUO) (2) investigation effects C5a receptor antagonist (C5aRA) UUO. In mice, when compared with controls, markers (Sirius Red, type I collagen, fibronectin, alpha-smooth muscle actin, vimentin, infiltrating...
Bacteriophage-derived endolysins have gained increasing attention as potent antimicrobial agents and numerous publications document the in vivo efficacy of these enzymes various rodent models. However, little has been documented about their safety toxicity profiles. Here, we present preclinical data for two pneumococcal endolysins, Pal Cpl-1. Microarray, gene profiling was performed on human macrophages pharyngeal cells exposed to 0.5 µM each endolysin six hours no change expression noted....
Arresting or regressing kidney scarring is of major clinical relevance. Platelet-derived growth factor D (PDGF-D) widely expressed in fibrotic kidneys. Administration the PDGF-D neutralizing fully human monoclonal antibody CR002 acute phase progressive anti-Thy 1.1 glomerulonephritis reduced glomerular and secondary tubulointerstitial damage.Using this model, we now assessed effects (n=15) vs irrelevant control IgG (n=17) administered on days 17, 28 35 after disease induction, i.e. damage...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally; recognition of immune responses to this virus will be crucial for disease 2019 (COVID-19) control, prevention and treatment. We comprehensively analysed IgG IgA antibody the SARS-CoV-2 nucleocapsid protein (N), spike domain 1 (S1) envelope (E) in: SARS-CoV-2-infected patient, healthy, historical pre-epidemic samples, including patients’ medical, epidemiological diagnostic data, virus-neutralizing capability...
Background. Renoprotective actions of angiotensin receptor blockers are not well established in normotensive, low-grade proteinuric glomerular diseases. We examined the effect low-dose telmisartan (LT) and high-dose (HT) versus conventional antihypertensive therapy rat anti-Thy1.1 model glomerulonephritis. Methods. Rats were randomized on Day 4 after disease induction to no treatment (CT, control), LT or HT hydrochlorothiazide + hydralazine (HCT H). Results. All rats remained normotensive:...
The neuropeptide Y (NPY) Y4 receptor is a G protein coupled receptor, which targeted by pancreatic polypeptide, homologue of NPY. Selective Y4R agonists were suggested as potential therapeutics for the treatment obesity. Highly potent dimeric peptidic agonists, constituting two pentapeptide moieties connected through an aliphatic linker, represent interesting class ligands. Based on this compound class, photoresponsive ligands, containing azobenzene, azopyrazole, diethienylethene or...
Abstract Background Lupus nephropathy (LN) occurs in approximately 50% of patients with systemic lupus erythematosus (SLE), and 20% them will eventually progress into end-stage renal disease (ESRD). A clinical tool predicting remission proteinuria might be utmost importance. In our work, we focused on the chance complete achievement LN patients, using artificial intelligence models, especially an neural network, called multi-layer perceptron. Methods It was a single centre retrospective...
The early asymptomatic stage of glomerular injury is a diagnostic challenge in the course renal and extra-renal disease, e.g., heart insufficiency. It was found that podocin, podocyte-specific protein present urine, may serve as biomarker diagnosis disease humans animals including glomerulonephritis, glomerulosclerosis, amyloidosis, or nephropathy. Therefore, there need development sensitive straightforward method urinary podocin identification. In this work, we report our extended research...
Delayed-graft function (DGF) might be responsible for shorter graft survival. Therefore, a clinical tool predicting its occurrence is vital the risk assessment of transplant outcomes. In single-center study, we conducted data mining and machine learning experiments, resulting in DGF predictive models based on random forest classifiers (RF) an artificial neural network called multi-layer perceptron (MLP). All designed had four common input parameters, determining best accuracy discriminant...
We are overwhelmed by a deluge of data and, although its interpretation is challenging, fortunately, information technology comes to the rescue. One tools artificial intelligence, allowing identification relationships between variables and their arbitrary classification. focused on assessment both remission proteinuria deterioration kidney function in patients with IgA nephropathy, comparing several methods machine learning. It utmost importance respond subtle changes function, which will...
Mesangioproliferative glomerulonephritis is the most common nephritis worldwide. We examined effects of low- and high-dose telmisartan, an angiotensin II receptor blocker, in rats with progressive anti-Thy1.1 mesangioproliferative a clinically relevant situation established renal damage. Uninephrectomized nephritic were randomized on day 28 to remain untreated (control treatment; CT), or receive (0.1 mg/kg/day, LT) telmisartan (10 HT), hydrochlorothiazide + hydralazine (8 32 HCT H), atenolol...
IgA nephropathy (IgAN) is the most frequent primary glomerulonephritis worldwide. Due to its heterogenicity, there a need establish robust biomarkers for IgAN, support treatment decisions and evaluate risk of progression end-stage renal disease. Using both clinical histopathological data, derived from biopsies, we aimed find predictors function deterioration proteinuria reduction. Clinical data 80 patients with biopsy proven IgAN were analyzed. In multivariate logarithmic regression model,...
High complexity of cell and tissue proteomes limits the investigation proteomic biomarkers. Therefore, methods enrichment some chemical groups peptides including thiopeptides are important tools that may facilitate analysis by reducing sample increasing proteome coverage. Here, we present a new method cysteine-containing tryptic peptide using commercially available TentaGel R RAM resin modified linker containing maleimide group, allowing thiol conjugation. The captured lysine residue were...
Kidneys play a crucial role in maintaining metabolic homeostasis body. Serum creatinine concentration is simple test used as an indicator of renal function. One the known ways quantifying liquid chromatography-mass spectrometry (LC-MS) method, using isotopically labeled analog internal standard. Unfortunately, such isotope-labeled analogs are expensive and their synthesis complex. Here we demonstrate facile preparation deuterated analogues creatinine, via H/D exchange hydrogens located at...