A5107976326- interferon and immune responses
- Pancreatic and Hepatic Oncology Research
- Radiomics and Machine Learning in Medical Imaging
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Nanoparticle-Based Drug Delivery
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Neuroendocrine Tumor Research Advances
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- Lung Cancer Research Studies
- Inflammasome and immune disorders
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Ubiquitin and proteasome pathways
Hudson Institute of Medical Research
2014-2023
Monash University
2012-2023
Rogers (United States)
2021-2023
Oregon State University
2021-2023
John Wiley & Sons (United Kingdom)
2019-2023
Hudson Institute
2009-2023
University of Hull
2019-2023
Emory University
2013-2017
Michigan State University
2014
Moscow Engineering Physics Institute
2014
Abstract Activating transcription factor-3 (ATF3) is rapidly induced by LPS in mouse macrophages and regulates TLR4 responses. We show that ATF3 various TLRs plasmacytoid dendritic cells (DCs), as well myeloid subsets of human DCs. In primary from mice with a targeted deletion the atf3 gene (ATF3-knockout (KO)), TLR-stimulated levels IL-12 IL-6 were elevated relative to responses wild-type macrophages. Similarly, correlated enhanced responsiveness DCs TLR activation measured secretion....
Fine-tuning of inflammatory responses by microRNAs (miRNAs) is complex, as they can both enhance and repress expression pro-inflammatory mediators. In this study, we investigate following global miRNA depletion, to better define the overall contribution miRNAs inflammation. We demonstrate that positively regulate Toll-like receptor signaling using inducible Dicer1 deletion depletion. establish an important miR-19b in effect, which potentiates nuclear factor-κB (NF-κB) activity human mouse...
Abstract Human TLR7 and 8 (hTLR7/8) have been implicated in the sequence-dependent detection of RNA oligonucleotides immune cells. Although hTLR7 sequence-specific sensing short RNAs has inferred from studies murine TLR7, this yet to be established for hTLR7. We found that different ssRNA sequences selectively induced either TNF-α or IFN-α human PBMCs. The response ssRNAs observed PBMCs could replicated activated macrophage-like (THP-1) cells pretreated with IFN-γ. Surprisingly, suppression...
MicroRNA-155 (miR-155) is highly expressed in many cancers such as B cell lymphomas and myeloid leukemia inflammatory disorders rheumatoid arthritis, atopic dermatitis, multiple sclerosis. The role of miR-155 both a promoter inflammation an oncogenic agent provides clear need for itself to be stringently regulated. We therefore investigated the transcriptional regulation response respective pro- anti-inflammatory mediators LPS IL-10. Bioinformatic analysis revealed Ets binding sites on...
To date, the activities of protein kinases have formed core our understanding cell signal transduction. Comprehension extent acetylation has raised expectations that this alternate post-transcriptional modification will be shown to rival phosphorylation in its importance mediating cellular responses. However, limited instances been identified. Here we show signalling from Toll-like or TNF-α receptors triggers calcium/calmodulin-dependent kinase (CaMK2) activate histone acetyltransferase-1...
Short-interfering RNAs (siRNAs) have engendered much enthusiasm for their ability to silence the expression of specific genes. However, it is now well established that siRNAs, depending on sequence, can be variably sensed by innate immune system through recruitment toll-like receptors 7 and 8 (TLR7/8). Here, we aimed identify sequence-based modifications allowing design bifunctional siRNAs with both proinflammatory silencing activities, potentially increased therapeutic benefits. We found...
Human Toll-like receptors (TLRs) TLR7, TLR8, and TLR9 are important immune sensors of foreign nucleic acids encountered by phagocytes. Although there is growing evidence implicating TLR7 in the detection intracellular pathogenic bacteria, characterization such a role for TLR8 currently lacking. A recent genetic study has correlated presence single nucleotide polymorphism (SNP) (rs3764880:A>G; p.Met1Val) with development active tuberculosis, suggesting phagosomal bacteria. Here we provide...
Recent studies have established that mutations or deletions in microRNA (miRNA) processing enzymes resulting a global decrease of miRNA expression are frequent across cancers and can be associated with poorer prognosis. While very popular profiling studies, it remains unclear whether microarrays suited not to accurately detecting decreases seen cancers. In this work, we analyzed the profiles samples using Affymetrix following inducible genetic deletion Dicer1 . Surprisingly, up third...
Significance Maintaining physiological balance is vital in the primary response to infectious and other stress stimuli avert damaging inflammation. Delineation of cell regulatory processes that control inflammatory better enable development informed strategies treat associated pathologies. Toward this end, we identify promyelocytic leukemia zinc finger (PLZF) transcription factor limits pathogen-induced PLZF stabilizes a repressor complex encompasses histone deacetylase activity, which...
Heterogeneity in terms of tumor characteristics, prognosis, and survival among cancer patients is an unsolved issue. Here, we systematically analyzed the aberrant expression patterns cervical using RNA-Seq data from The Cancer Genome Atlas (TCGA). We incorporated gene profiling, molecular signatures, functional pathway information with set enrichment protein-protein interaction (PPI) network analysis, to identify sub-networks genes. Those identified genes relating DNA replication...
Gene-recombinase technologies, such as Cre/loxP-mediated DNA recombination, are important tools in the study of gene function, but have potential side effects due to damaging activity on DNA. Here we show that recombination by Cre instigates a robust antiviral response mammalian cells, independent legitimate loxP recombination. This is recruitment cytosolic sensor STING, concurrent with Cre-dependent damage and accumulation cytoplasmic Importantly, establish direct interplay between this...
Acridine dyes, including proflavine and acriflavine, were commonly used as antiseptics before the advent of penicillins in mid-1940s. While their mode action on pathogens was originally attributed to DNA intercalating activity, work early 1970s suggested involvement host immune responses, characterized by induction interferon (IFN)-like activities through an unknown mechanism. We demonstrate here that sub-toxic concentrations a mixture acriflavine instigate cyclic-GMP-AMP (cGAMP) synthase...
Inflammatory responses, while essential for pathogen clearance, can also be deleterious to the host. Chemical inhibition of topoisomerase 1 (Top1) by low-dose camptothecin (CPT) suppress transcriptional induction antiviral and inflammatory genes protect animals from excessive damaging responses. We describe unexpected finding that minor DNA damage with CPT trigger a strong immune response through cyclic GMP-AMP synthase (cGAS) detection cytoplasmic DNA. This argues against having only...