A5079450192- Viral Infections and Immunology Research
- Aquaculture Nutrition and Growth
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- Biological Research and Disease Studies
- Animal Virus Infections Studies
- Nanoparticle-Based Drug Delivery
- SARS-CoV-2 detection and testing
- Trace Elements in Health
- Aluminum toxicity and tolerance in plants and animals
- Veterinary Oncology Research
- Microscopic Colitis
- Wound Healing and Treatments
- RNA and protein synthesis mechanisms
- Diabetic Foot Ulcer Assessment and Management
- Herbal Medicine Research Studies
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Oral and Maxillofacial Pathology
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Nonmelanoma Skin Cancer Studies
Max Perutz Labs
2005
Medical University of Vienna
2002-2005
Vienna Biocenter
2005
Ulcerous lesions can arise in primary skin cancers and upon infiltration of the by malignant cells originating from other organs. These fungating wounds are difficult to treat, they cause pain, itching malodor. Distressing malodor imposes a major burden on patients. The carrion odor decaying tissue is—at least part—due bacterial breakdown products cadaverine putrescine. Here, we examined binding cadaverine, histamine, putrescine, spermidine spermine preparation micronized purified...
In an ageing society, chronic ulcers pose increasingly relevant healthcare issue associated with significant morbidity and increasing financial burden. Hence, there is unmet medical need for novel, cost-effective therapies that improve healing of cutaneous wounds. This prospective, randomised, open-label, phase I trial investigated the safety tolerability topically administered purified clinoptilolite-tuff (PCT), mainly consisting naturally occurring zeolite-mineral clinoptilolite, in...
Clostridioides difficile (C . difficile) infection is a major public health problem worldwide. The current treatment of C -associated diarrhea relies on the use antibacterial agents. However, recurrences are frequent. main virulence factors two secreted cytotoxic proteins toxin A and B. Alternative research exploring binding by resins found reduced rate recurrence administration tolevamer. Hence, exotoxins may be useful in preventing relapse provided that adsorbent innocuous. Here, we...
Due to its remarkable surface properties, natural clinoptilolite-tuff interacts with a variety of biochemical, pharmaceutical, chemical, and microbiological entities, including human viruses. In the present work, virucidal activity purified (PCT) was investigated using viruses, differing in their structure composition. Influenza A virus, Herpes Simplex Rhinovirus, Parvovirus were chosen represent enveloped non-enveloped viruses RNA DNA genomes. Beside Canine bacteriophages T4 MS2 used animal...
The current COVID19 pandemic is caused by a positive-sense single-stranded RNA virus, which presents high mutational rates. development of effective therapeutics and mitigation strategies using vaccination or therapeutic antibodies faces serious challenges because the regular emergence immune escape variants virus. An efficient approach would involve use an agent to non-specifically limit block viruses contacting mucosae therefore entering body. Here, we investigated ability micronized...
In vivo, the icosahedral capsid of human rhinoviruses undergoes well-defined transitions during infection pathway. Native virus, sedimenting at 150S, is converted to subviral particles with a sedimentation coefficient 135S, which have lost innermost protein VP4. Upon release genomic RNA empty 80S capsids remain. Similar structural modifications are observed in vitro upon exposure low pH and/or elevated temperature. Virions stabilized against these by various antiviral compounds, bind...
Abstract Background Clinoptilolite is an aluminium silicate of natural origin; the microporous structure and net negative charge its crystal lattice allows for adsorption ions, toxins, inflammatory mediators, some microorganisms. We generated 2 preparations purified clinoptilolite, which differed by about 10-fold in particle size, ie, a standard powder (GHC1) microparticulate fraction (GHC2) with size 3.6 µm 0.39 (d50) respectively. These were examined their ability to accelerate recovery...
Minor group human rhinoviruses (HRVs) bind members of the low-density lipoprotein receptor family for cell entry. The ligand-binding domains these membrane proteins are composed various numbers direct repeats about 40 amino acids in length. Residues involved binding module 3 (V3) very-low-density (VLDLR) to HRV2 have been identified by X-ray crystallography (N. Verdaguer, I. Fita, M. Reithmayer, R. Moser, and D. Blaas, Nat. Struct. Mol. Biol. 11:429-434, 2004). Sequence comparisons eight...
Abstract Lack of contrast is a common problem encountered when doing TEM cultured cells, especially membranes. Using ferrocyanide as post-fixative can greatly improve membrane fixation and staining. This protocol has been used to study Caco-2 cells grown on PET membranes (the “Caco model”). colon cancer cell line that differentiates upon reaching confluency. allows permeability studies model, which reasonably similar the human intestine. Basically, classical, only peculiarity consisting in...