A5005668372- Cardiac Fibrosis and Remodeling
- Cardiac Structural Anomalies and Repair
- Tissue Engineering and Regenerative Medicine
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- Sirtuins and Resveratrol in Medicine
- Signaling Pathways in Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Lymphatic System and Diseases
- Cardiovascular Disease and Adiposity
- Cardiovascular Function and Risk Factors
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Protease and Inhibitor Mechanisms
- Cardiac Ischemia and Reperfusion
- Retinal Development and Disorders
- Genetics and Neurodevelopmental Disorders
- Zebrafish Biomedical Research Applications
- Cardiac electrophysiology and arrhythmias
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Nicotinic Acetylcholine Receptors Study
- Calcium signaling and nucleotide metabolism
University of Manitoba
2014-2023
St. Boniface Hospital
2012-2022
Research Manitoba
2014-2018
Cardiovascular Institute Hospital
2008-2018
Jeffrey Modell Foundation
2015
Manitoba Beekeepers' Association
2014
University of Ottawa
1994-2004
St. Jude Children's Research Hospital
1999-2002
Although the modulation of ion channel gating by hormones and drugs has been extensively studied, much less is known about how cell surface expression levels are regulated. Here, we demonstrate that density both heterologously expressed K+ encoded human ether-a-go-go-related gene (HERG) its native counterpart, rapidly activating delayed rectifier (IKr), in rabbit hearts vivo precisely controlled extracellular concentration ([K+]o) within a physiologically relevant range. Reduction [K+]o led...
In response to pro-fibrotic signals, scleraxis regulates cardiac fibroblast activation in vitro via transcriptional control of key fibrosis genes such as collagen and fibronectin; however, its role vivo is unknown. The present study assessed the impact loss on activation, fibrosis, dysfunction pressure overload-induced heart failure.Scleraxis expression was upregulated hearts non-ischemic dilated cardiomyopathy patients, mice subjected overload by transverse aortic constriction (TAC)....
Dietary flaxseed has significant anti-atherogenic effects. However, the limits of this action and its effects on vascular contractile function are not known. We evaluated supplementation atherosclerosis under prolonged hypercholesterolemic conditions in New Zealand White rabbits assigned to one four groups for 6, 8, or 16 wk feeding: regular diet (RG), 10% flaxseed-supplemented (FX), 0.5% cholesterol-supplemented (CH), cholesterol- (CF). Cholesterol feeding resulted elevated plasma...
Fibroblast growth factor 2 (FGF-2) is a multifunctional protein synthesized as high (Hi-) and low (Lo-) molecular weight isoforms. Studies using rodent models showed that Hi- Lo-FGF-2 exert distinct biological activities: after myocardial infarction, rat Lo-FGF-2, but not Hi-FGF-2, promoted sustained cardioprotection angiogenesis, while hypertrophy reduced contractile function. Because there no information regarding Hi-FGF-2 in human myocardium, we undertook to investigate expression,...
// Shivika S. Gupta 1 , Matthew R. Zeglinski Sunil G. Rattan Natalie M. Landry Saeid Ghavami 2, 3 Jeffrey T. Wigle 5 Thomas Klonisch 2 Andrew J. Halayko 3, 4 Ian M.C. Dixon Department of Physiology and Pathophysiology, Institute Cardiovascular Sciences, Rady Faculty Health Max College Medicine, University Manitoba, Winnipeg, Canada Human Anatomy Cell Science, Basic Medical Sciences Building, Children’s Hospital Research John Buhler Centre, Internal Medicine Pediatrics Child Health,...
Senescence, the state of permanent cell cycle arrest, has been associated with endothelial dysfunction and atherosclerosis. The cyclin dependent kinase inhibitors p21CIP1/WAF1 p16INK4a govern G1/S checkpoint are essential for determining whether a enters into an arrested state. homeodomain transcription factor MEOX2 is important regulator vascular proliferation direct transcriptional activator both p16INK4a. MEOX1 have shown to be partially functionally redundant during development,...
Cardiac fibrosis is linked to fibroblast-to-myofibroblast phenoconversion and proliferation but the mechanisms underlying this are poorly understood. Ski a negative regulator of TGF-β-Smad signaling in myofibroblasts, might redirect myofibroblast phenotype back fibroblasts. Meox2 could alter TGF-β-mediated cellular processes repressed by Zeb2. Here, we investigated whether diminishes de-repressing expression function through repression Zeb2 expression. We show that Meox1 mRNA protein reduced...
In cardiac wound healing following myocardial infarction (MI), relatively inactive resident fibroblasts phenoconvert to hypersynthetic/secretory myofibroblasts that produce large quantities of extracellular matrix (ECM) and fibrillar collagen proteins. Our laboratory others have identified TGFβ1 as being a persistent stimulus in the chronic inappropriate phase is marked by hypertrophic scarring eventual stiffening entire myocardium, ultimately leading pathogenesis heart failure MI. Ski...
The phenotype conversion of fibroblasts to myofibroblasts plays a key role in the pathogenesis cardiac fibrosis. Numerous triggers this process have been identified, including plating cells on solid substrates, cytokines such as transforming growth factor-β, and mechanical stretch; however, underlying mechanisms remain incompletely defined. Recent studies from our laboratory revealed that transcription factor scleraxis is regulator fibroblast extracellular matrix expression. Here we report...
Regulated retinal ganglion cell (RGC) differentiation and axonal guidance is required for a functional visual system. Homeodomain basic helix loop transcription factors are retinogenesis, as well patterning, maintenance of specific types. We hypothesized that Dlx1/Dlx2 Brn3b homeobox genes function in parallel intrinsic pathways to determine RGC fate generated Dlx1/Dlx2/Brn3b triple knockout mice. A more severe phenotype was found the null retinas than predicted by combining features single...
The expression of the dihydropyridine (DHP) and ryanodine receptors in skeletal muscle was investigated during development rat myotubes culture as well embryonic postnatal rat. Through use specific gene probes, antibodies radioligand binding ([3H]PN 200-110 [3H]ryanodine), we identified a significant difference between time course appearance DHP receptor development. Although number dramatically increased at early stages (up to day 7 tissue 20 postnatal), increase density occurred...