A biocompatible hydrogel consisting of extracellular matrix (ECM) from human tendons is described as a potential scaffold for guided tissue regeneration and engineering purposes. Lyophilized decellularized were milled enzymatically digested to form an ECM solution. The solution properties are assessed by proteome analysis with mass spectrometry, the material's rheological determined function frequency, temperature, time. In vivo application gel in rat model remodeling host cell repopulation....

Introduction Microvascular changes in the skin due to pharmacological and physiological provocations can be used as a marker for vascular function. While laser Doppler flowmetry (LDF) has been extensively measurement of microvascular responses, Laser Speckle Contrast Imaging (LSCI) Tissue Viability (TiVi) are novel imaging techniques. TiVi measures red blood cell concentration, while LDF LSCI measure perfusion. Therefore, aim this study was compare responses using these different Method...

Background Laser Speckle Contrast Imaging (LSCI) is a non-invasive and fast technique for measuring microvascular blood flow that recently has found clinical use burn assessment evaluation of flaps. Tissue motion caused by example breathing or patient movements may however affect the measurements in these applications, as distance between camera skin tissue curvature. Therefore, aims this study were to investigate effect frame rate, number frames/image, movement tissue, curvature on measured...

Abstract Objective . Electrical stimulation of the peripheral nervous system (PNS) can treat various diseases and disorders, including healing process after nerve injury. A major challenge when designing electrodes for PNS is mechanical mismatch between device, which lead to non-conformal contact, tissue damage inefficient due current leakage. Soft stretchable cuff promise tackle these challenges but often have limited performance rely on unconventional materials. The aim this study develop...

PurposeThe aim of this study was to evaluate the effect reconstruction parameters on image quality in wrist imaging using photon-counting detector CT (PCD-CT) and compare results with images from an energy-integrating (EID-CT).MethodsTwelve cadaveric specimens were examined a prototype PCD-CT clinical EID-CT similar radiation dose. Reconstruction matched between scanners. Also, sharper kernels, larger matrix size, smaller slice thicknesses evaluated for PCD-CT. Image noise, contrast-to-noise...

Many unsolved problems in plastic and hand surgery are related to poor healing of acute chronic tendon injuries. The authors hypothesized that could be augmented by the addition a tendon-derived, extracellular matrix hydrogel would guide tissue regeneration.Both Achilles tendons 36 Wistar rats were given full-thickness injuries approximately 5 mm long 0.5 wide from insertion at calcaneus midsubstance. was injected into injury site one leg compared with control saline other. ultimate failure...

The aim of this study was to analyse the short- and medium-term complications Motec total wrist arthroplasty (TWA). Identifying exact modes failure their causes should allow surgeons avoid or mitigate these risks in future. Retrospective analysis prospectively collected data from six hand at five international centres provided details 171 TWAs. mean follow-up 5.8 years (range 18 months 12 years). There were 33 (19%) within our cohort, with a revision rate 8.2% (14 revisions). no difference...

The aim of this Delphi study was to provide a diagnostic and treatment algorithm for patients with persistent or recurrent symptoms after trapeziometacarpal joint resection arthroplasty. Three rounds were conducted in which surveys sent 182 experienced hand surgeons worldwide. Responses received from 140 participants. A consensus threshold set at 67% agreement. Diagnostic tools approaches six common revision scenarios achieved consensus. Radiographs are appropriate as primary (97%) CT scans...

Optimal recovery of muscle function after proximal nerve injuries remains a complex and challenging problem. After injury, alterations in the affected muscles lead to atrophy, later degeneration replacement by fat-fibrous tissues. At present, several different strategies for preservation skeletal have been reported, including various sets physical exercises, massage, methods (e.g. electrical stimulation, magnetic field laser low-intensity pulsed ultrasound), medicines nutrients, natural...

Abstract Bioelectronic medicine can treat diseases and disorders in humans by electrically interfacing with peripheral nerves. Multielectrode cuffs be used for selective stimulation of portions the nerve, which is advantageous treatment specificity. The biocompatibility conformability improved reducing mechanical mismatch between nerve tissue cuffs, but nerves has yet to achieved soft stretchable cuff electrodes. Here, this paper reports development a multielectrode (sMEC) stimulation....

Abstract Postmastectomy radiotherapy (PMRT) increases the risk for complications after breast reconstruction. The pathophysiological mechanism underlying this increased is not completely understood. aim of study was to examine if there a relationship between PMRT and microvascular perfusion in skin directly after, at 2 6 months assess impaired responsiveness topically applied vasodilator (Methyl nicotinate—MN) PMRT. Skin responses were measured on two sites irradiated chest wall 22 women...

Cadaveric tendon allografts form a readily available and underutilized source of graft material. Because their material properties, are biomechanically biologically superior to synthetic scaffolds. However, before clinical use, must undergo decellularization reduce immunogenicity oxidation increase porosity, leaving nonvital biostatic scaffold. Ex vivo seeding, or revitalization, is thought hasten incorporation stimulate intrinsic healing, permitting early mobilization return function. In...

Tissue-engineered human flexor tendons may be an option to aid in reconstruction of complex upper extremity injuries with significant tendon loss. The authors hypothesize that adipose-derived stem cells remain viable following reseeding on scaffolds vivo and graft integration.Decellularized harvested from fresh-frozen cadavers reseeded green fluorescent protein-labeled pooled were examined bioluminescent imaging immunohistochemistry. Reseeded repaired compared biomechanically unseeded...

Background: Restoration of biomechanical strength following surgical reconstruction tendon or ligament insertion tears is challenging because these injuries typically heal as fibrous scars. The authors hypothesize that at the tendon-bone interface would benefit from with decellularized composite grafts. Methods: Tendon-bone grafts were harvested Sprague-Dawley rats. Grafts subjected to decellularization compared histologically and biomechanically untreated ex vivo in a new model. Wistar rats...

The aim of this study was to identify risk factors for reoperations after Zones 1 and 2 flexor tendon repairs. A multiple logistic regression model used from data collected via the Swedish national health care registry hand surgery (HAKIR). studied potential were age gender, socio-economics surgical techniques. Included 1372 patients with injuries 1585 fingers follow-up at least 12 months (median 37 IQR 27-56). Tendon ruptures occurred in 80 tenolysis required 76 fingers. Variables that...

Extremity injuries involving tendon attachment to bone are difficult address. Clinically, tendon-bone interface allografts must be decellularized reduce immunogenicity. Composite grafts decellularize because chemical agents cannot reach cells between tissues. In this study, the authors attempted optimize graft decellularization.Human flexor digitorum profundus tendons with attached distal phalanx were harvested from cadavers and divided into four groups. Group 1 (control) was untreated. 2...

The aim of this study was to evaluate the urea clearance technique for measurement drug-induced blood flow changes in human skin and compare it two non-invasive techniques: polarization light spectroscopy laser Doppler perfusion imaging.Fifteen microdialysis catheters were placed intracutaneously on volar aspect forearms healthy subjects perfused with nitroglycerine, noradrenaline, again nitroglycerine induce local tissue hyperemia, hypoperfusion, respectively.Urea clearance, but not other...

Background: Tendon-derived extracellular matrix (ECM) hydrogel has been shown to augment tendon healing in vivo. We hypothesized that reseeding of the gel with adipose-derived stem cells (ASCs) could further assist repopulation and combinations growth factors (GFs) would improve survival these after reseeding. Methods: A tendon-specific ECM solution was supplemented varying concentrations basic fibroblast factor (bFGF), insulin-like factor-1 (IGF-1), platelet-derived factor-BB (PDGF-BB)....

The majority of breast cancer patients who receive radiation treatment are affected by acute radiation-induced skin changes. assessment these changes is usually done subjective methods, which complicates the comparison between different treatments or patient groups. This study investigates feasibility new robust methods for monitoring microcirculation to objectively assess and quantify reactions during treatment.Laser Doppler flowmetry, laser speckle contrast imaging, polarized light...