A5009882011- Genomics and Chromatin Dynamics
- Smoking Behavior and Cessation
- RNA Research and Splicing
- RNA modifications and cancer
- Biochemical Analysis and Sensing Techniques
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Advanced Chemical Sensor Technologies
- Epigenetics and DNA Methylation
- Global Public Health Policies and Epidemiology
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- Consumer Attitudes and Food Labeling
- Agricultural safety and regulations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Effects and risks of endocrine disrupting chemicals
- Nicotinic Acetylcholine Receptors Study
- Meat and Animal Product Quality
- RNA and protein synthesis mechanisms
- Animal testing and alternatives
- Computational Drug Discovery Methods
- Sensory Analysis and Statistical Methods
- Chromosomal and Genetic Variations
- IL-33, ST2, and ILC Pathways
- Diabetes and associated disorders
National Institute for Public Health and the Environment
2015-2018
Eindhoven Cancer Registry
2018
Janssen (Netherlands)
2018
Johnson & Johnson (Netherlands)
2018
Max Planck Institute of Immunobiology and Epigenetics
2012-2017
University of Freiburg
2013
Erasmus MC
2008-2012
Erasmus University Rotterdam
2008-2009
The Suv39h1 and Suv39h2 histone lysine methyltransferases are hallmark enzymes at mammalian heterochromatin. We show here that the mouse enzyme differs from by containing an N-terminal basic domain facilitates retention mitotic chromatin provides additional affinity for major satellite repeat RNA. To analyze RNA-dependent interaction with chromatin, we purified native nucleosomes ES cells detect exclusively associate poly-nucleosomes. This association was attenuated upon RNaseH incubation...
CCCTC binding factor (CTCF) is a highly conserved zinc finger protein, which involved in chromatin organization, local histone modifications, and RNA polymerase II-mediated gene transcription. CTCF may act by tightly to DNA recruiting other proteins mediate its various functions the nucleus. To further explore role of this essential factor, we used mass spectrometry-based approach screen for novel CTCF-interacting partners.Using biotinylated as bait, identified upstream (UBF) multiple...
CTCF is a highly conserved and essential zinc finger protein expressed in virtually all cell types. In conjunction with cohesin, it organizes chromatin into loops, thereby regulating gene expression epigenetic events. The function of CTCFL or BORIS, the testis-specific paralog CTCF, less clear.Using immunohistochemistry on testis sections fluorescence-based microscopy intact live seminiferous tubules, we show that only transiently present during spermatogenesis, prior to onset meiosis, when...
Abstract Differentiation of naive CD4+ cells into Th2 is accompanied by chromatin remodeling at the cytokine locus allowing expression IL-4, IL-5, and IL-13 genes. In this report, we investigated role in differentiation transcription regulator CCCTC-binding factor (CTCF). Chromatin immunoprecipitation analysis revealed multiple CTCF binding sites locus. Conditional deletion Ctcf gene double-positive thymocytes allowed development peripheral T cells, but their activation proliferation upon...
Objectives Tobacco flavours are an important regulatory concept in several jurisdictions, for example the USA, Canada and Europe. The European Products Directive 2014/40/EU prohibits cigarettes roll-your-own tobacco having a characterising flavour. This directive defines flavour as ‘a clearly noticeable smell or taste other than one of […]’. To distinguish between products with without flavour, we trained expert panel to identify by smelling. Methods An (n=18) evaluated 20 using self-defined...
Background: A wide variety of new tobacco and tobacco-related products have emerged on the market in recent years.