A5027094361- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Genetics, Bioinformatics, and Biomedical Research
- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Biomedical and Engineering Education
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Gene expression and cancer classification
- Genomics and Phylogenetic Studies
- Noise Effects and Management
- Vehicle Noise and Vibration Control
- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Cancer-related gene regulation
- Interdisciplinary Research and Collaboration
- Social and Educational Sciences
- Insect symbiosis and bacterial influences
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- RNA modifications and cancer
Science for Life Laboratory
2016-2023
Stockholm University
2016-2022
National Bioinformatics Infrastructure Sweden
2020
Karolinska Institutet
2002-2016
Karolinska University Hospital
2004-2016
Akershus University Hospital
2016
University of Oslo
2007-2013
University of Gothenburg
2013
We sought to determine whether acute resistance exercise (RE)-induced gene expression is modified by RE training. studied the patterns of a select group genes following an bout in naïve and hypertrophying muscle. Thirteen untrained subjects underwent supervised training for 12 wk nondominant arm performed 1 after last program ( training+acute). The dominant was either unexercised control) or subjected same as trained RE). Following training, men (14.8 ± 2.8%; P < 0.05) women (12.6 2.4%;...
Epigenetic modifications contribute to the etiology of type 2 diabetes.We performed genome-wide methylome and transcriptome analysis in liver from severely obese men with or without diabetes non-obese discover aberrant pathways underlying development insulin resistance. Results were validated by pyrosequencing.We identified hypomethylation genes involved hepatic glycolysis resistance, concomitant increased mRNA expression protein levels. Pyrosequencing revealed CpG-site within ATF-motifs was...
Despite a considerable focus on the adverse effects of silver nanoparticles (AgNPs) in recent years, studies potential long-term AgNPs are scarce. The aim this study was to explore following repeated low-dose, exposure human bronchial epithelial cells. To end, BEAS-2B cell line exposed 1 µg/mL (10 nm) for 6 weeks followed by RNA-sequencing (RNA-Seq) as well genome-wide DNA methylation analysis. transcriptomics analysis showed that substantial number genes (1717) were differentially expressed...
Abstract Background Chronic fatigue syndrome (CFS) is a prevalent and disabling condition affecting adolescents. The pathophysiology poorly understood, but immune alterations might be an important component. This study compared whole blood gene expression in adolescent CFS patients healthy controls, explored associations between neuroendocrine markers, markers clinical within the group. Methods (12–18 years old) were recruited nation-wide to single referral center as part of NorCAPITAL...
Abstract Background Innate immune responses are evolutionarily conserved processes that provide crucial protection against invading organisms. Gene activation by potent NF-κB transcription factors is essential both in mammals and Drosophila during infection stress challenges. If not strictly controlled, this defense system can activate autoimmune inflammatory reactions, with deleterious consequences for the organism. Negative regulation to prevent gene healthy organisms, presence of...
Transcription-blocking oxidative DNA damage is believed to contribute aging and underlie activation of stress responses downregulation insulin-like signaling (ILS) in Nucleotide Excision Repair (NER) deficient mice.Here, we present the first quantitative proteomic description Caenorhabditis elegans NER-defective xpa-1 mutant compare proteome transcriptome signatures.Both methods indicated responses, which was substantiated biochemically by a bioenergetic shift involving increased...
Abstract T cells expressing the cutaneous lymphocyte antigen (CLA) mediate pathogenic inflammation in atopic dermatitis (AD). The molecular alterations contributing to their dysregulation remain unclear. With aim elucidate putative altered pathways AD we profiled DNA methylation levels and miRNA expression sorted cell populations (CD4 + , CD4 CD45RA naïve, CLA CD8 ) from adult patients healthy controls (HC). Skin homing showed significant differences 40 genes compared HC ( p < 0.05)....
The cyclin-dependent kinase Cdc28 is the master regulator of cell cycle in Saccharomyces cerevisiae . initiates by activating cell-cycle–specific transcription factors that switch on a transcriptional program during late G1 phase. also has cell-cycle–independent, direct function regulating basal transcription, which does not require its catalytic activity. However, exact role remains poorly understood, and for activity been fully explored. Here we show important transcription. Using...
Human-nucleotide-excision repair (NER) deficiency leads to different developmental and segmental progeroid symptoms of which the pathogenesis is only partially understood. To understand biological impact accumulating spontaneous DNA damage, we studied phenotypic consequences DNA-repair in Caenorhabditis elegans. We find that damage accumulation does not decrease adult life span post-mitotic tissue. Surprisingly, loss functional ERCC-1/XPF even further extends long-lived daf-2 mutants, likely...
Summary Bruton's tyrosine kinase (Btk), a member of the Tec family kinases, plays an important role in differentiation and activation B cells. Mutations affecting Btk cause immunodeficiency both humans mice. In this study we set out to investigate potential Toll‐like receptor 9 (TLR9) production pro‐inflammatory cytokines such as interleukin (IL)‐6, tumour necrosis factor (TNF)‐α IL‐12p40. Our data show that Btk‐deficient cells respond more efficiently CpG‐DNA stimulation, producing...
Curriculum development in higher education should follow a formal process. Although the focus curriculum theory is on long-term programs of study, theoretical and practical considerations are also applicable to shorter-form learning experiences (single courses, lessons, or training sessions). With these mind, we discuss here an iterative model design, starting point which (indeed, construction any experience), articulation target outcomes: everything follows from these, including selection...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a key regulator of serum cholesterol. The possibility that PCSK9 also functions in other pathways needs to be addressed. We have transfected HepG2 cells with mutant D374Y-PCSK9 or control vector. Gene expression signatures were determined using the Affymetrix GeneChip technology, and pattern selected genes was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Data normalized analyzed model-based background...
Cyclin-dependent kinases (CDKs) control the eukaryotic cell cycle, and a single CDK, Cdc28 (also known as Cdk1), is necessary sufficient for cycle regulation in budding yeast Saccharomyces cerevisiae . regulates cycle-dependent processes such transcription, DNA replication repair, chromosome segregation. To gain further insight into functions of Cdc28, we performed high-throughput chemical-genetic array (CGA) screen aimed at unraveling genetic network CDC28 We identified 107 genes that...
The neural crest is a transient embryonic stem cell population. Hypoxia inducible factor (HIF)-2α associated with appearance and aggressiveness in tumors. However, little known about its role normal development.Here, we show that HIF-2α expressed trunk cells of human, murine, avian embryos. Knockdown as well overexpression vivo causes developmental delays, induces proliferation, self-renewal capacity while decreasing the proportion migrate ventrally to sympathoadrenal sites. Reflecting...
ABSTRACT Science, technology, engineering, mathematics, and medicine (STEMM) fields change rapidly are increasingly interdisciplinary. Commonly, STEMM practitioners use short-format training (SFT) such as workshops short courses for upskilling reskilling, but unaddressed challenges limit SFT’s effectiveness inclusiveness. Prior work, including the NSF 2026 Reinventing Scientific Talent proposal, called addressing SFT challenges, a diverse international group of experts in education,...
Activation of oxidative stress-responses and downregulation insulin-like signaling (ILS) is seen in Nucleotide Excision Repair (NER) deficient segmental progeroid mice. Evidence suggests that this a survival response to persistent transcription-blocking DNA damage, although the relevant lesions have not been identified. Here we show loss NTH-1, only Base (BER) enzyme known initiate repair damage inC. elegans, restores normal lifespan short-lived NER xpa-1 mutant. Loss NTH-1 leads stress...
Developmental dyslexia is the most common learning disorder in children. Problems reading and writing are likely due to a complex interaction of genetic environmental factors, resulting reduced power studies factors underlying developmental dyslexia. Our approach current study was perform exome sequencing affected unaffected individuals within an extended pedigree with familial form We identified two-base mutation, causing p.R229L amino acid substitution centrosomal protein 63 kDa (CEP63),...
Gut immunity is regulated by intricate and dynamic mechanisms to ensure homeostasis despite a constantly changing microbial environment. Several regulatory factors have been described participate in feedback responses prevent aberrant immune activity. Little is, however, known about how transcriptional programs are directly tuned efficiently adapt host gut tissues the current microbiome. Here we show that POU/Oct gene nubbin (nub) encodes two transcription factor isoforms, Nub-PB Nub-PD,...
As the life sciences have become more data intensive, pressure to incorporate requisite training into life-science education and programs has increased. To facilitate curriculum development, various sets of (bio)informatics competencies been articulated; however, these proved difficult implement in practice. Addressing this issue, we created a curriculum-design -evaluation tool support development specific Knowledge, Skills Abilities (KSAs) that reflect scientific method promote both...